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Citations for ChromoTek :
101 - 150 of 177 citations for Recombinant Rat PCSK9 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... and rat monoclonal anti-RFP antibody (1:5,000; Chromotek), respectively.
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bioRxiv - Plant Biology 2020Quote: ... or c-Myc rat monoclonal antibody (Chromotek, 9E1-100) at a dilution of 1:1000 followed by goat anti-rat IgG horseradish peroxidase (Abcam ...
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Cellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona larvabioRxiv - Neuroscience 2020Quote: ... rat anti-red fluorescent protein (RFP) monoclonal (5F8; ChromoTek GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... anti-RFP (1:500, rat monoclonal, Chromotek, 5f8-100), anti-Doublecortin (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... TdTomato (rat monoclonal, Chromotek, 5F8, used at 1:500); TdTomato (rabbit polyclonal ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-RFP (1:1000, Chromotek, 5f8-100, RRID:AB_2336064); chicken anti-S100B (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: ... GFP-tagged proteins were immunoprecipitated by incubation with 25 µl of GFP-Trap magnetic agarose beads (Chromotek) overnight at 4 °C on a tube rotator ...
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bioRxiv - Cell Biology 2021Quote: ... cleared lysates containing GFP-tagged proteins were added to anti-GFP agarose beads (GFP-Trap, ChromoTek, Germany) and incubated while rotating end over end for an hour at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... A rat monoclonal anti-RFP antibody (Chromotek, cat. no. 5F8) was used at 1:1000 dilution and combined with a 1:5000 donkey anti-rat secondary antibody (Agrisera ...
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bioRxiv - Neuroscience 2022Quote: ... rat monoclonal anti-red fluorescent protein (RFP) (Chromotek, 1:1000) and goat anti-mCherry (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: ... Rat anti-RFP (1/500, v/v; Cat#5f8, Chromotek), Mouse anti-GFP (1/600 ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-myc (Chromotek, 9e1-100, 1/500 for ICC), chicken anti-GFP (AvesLab ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with anti-GFP antibodies (Rat monoclonal, ChromoTek) diluted 1/1500 in TBS containing 0.1% Tween overnight at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-RFP (Chromotek 5F8ChromoTek 5f8-100, WB: 1:1000); chicken anti-MAP2 (antibodies Antibodies-Online ABIN361345 ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Cell Biology 2020Quote: IPs with GFP-tagged proteins were performed with GFP-binding magnetic agarose beads (GFPtrap MA, Chromotek, #gtma-20). Per IP sample ...
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bioRxiv - Neuroscience 2022Quote: ... red fluorescent protein (RFP; rat, Chromotek: 5F8, RRID: AB_2336064; 1:2000) FoxP2 (mouse ...
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bioRxiv - Molecular Biology 2021Quote: ... rat anti-MYC (1:1000; Chromotek, Germany, Cat. No. 9e1-20) and rabbit anti-REGE-1 polyclonal antibody raised against the first 119 amino acids (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... rat anti-RFP (1:1000, Chromotek, Cat# 5f8-100, RRID: AB_2336064), rabbit anti-tomosyn (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Western blotting was performed using rat anti-mCherry (1:5,000; ChromoTek), chicken anti-GFP (1:5,000 ...
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bioRxiv - Plant Biology 2020Quote: ... anti-HA and anti-FLAG antibodies produced in rat (Chromotek, UK) were used as primary antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... rat anti-red fluorescent protein (RFP) (5F8, Chromotek, Planegg-Martinsried, Germany) (1:10,000) ...
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bioRxiv - Immunology 2021Quote: ... GFP-tagged PD-1 variants or BTLA variants were IP’ed from the lysate using GFP-Trap (Chromotek, #gta-20). Equal fractions of the IP samples were subjected to SDS-PAGE and blotted with indicated antibodies.
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bioRxiv - Cell Biology 2022Quote: ... MEFs were washed extensively and GFP-booster (tagged with Alexa488, ChromoTek, gb2AF488-50, 1:500 in 3%BSA/PBS) and anti-rabbit-Alexa568 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and the retained Flag-tagged FlaA/B was eluted from the agarose beads using 3xFLAG®-peptide (Chromotek, Proteintech). For the co-IP of GlfM from S ...
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bioRxiv - Neuroscience 2019Quote: ... or TdTomato (1:1000; rat; anti-RFP; Chromotek, CAT#:5f8-100, RRID:AB_2336064) and Goat-Anti-chicken AlexaFluor488 (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... or TdTomato (1:1000; rat; anti-RFP; Chromotek, CAT# 5f8-100, RRID:AB_2336064) or slices were co-stained with the nuclear marker 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Plant Biology 2021Quote: ... RFP detection was performed with a rat anti-RFP 5F8 antibody (Chromotek) and an HRP-conjugated anti-rat antibody ...
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bioRxiv - Cell Biology 2019Quote: ... The mCherry fluorescent signal was enhanced with rat mAb anti-RFP (Chromotek). As secondary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... Nitrocellulose membranes were probed with rat anti-GFP (3H9, Chromotek, 1:2,500), PAP (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Antibodies used were as follows: rat anti-RFP (Chromotek, 5F8; 1:200), rabbit anti-RFP (MBL ...
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bioRxiv - Biochemistry 2021Quote: Protein extracts containing GFP-tagged EFR or site-directed mutants were incubated with 20 μL of GFP-Trap beads (Chromotek) or GFP-clamp beads (Hansen et al. ...
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bioRxiv - Genetics 2020Quote: ... Clover- and Cherry-tagged proteins were immunoprecipitated by incubation with either GFP-(gta) or RFP-Trap(rta) magnetic beads/agarose (Chromotek) for 1 h at 4°C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... the extract prepared from OSC lines expressing endogenously tagged proteins of interest was incubated with magnetic GFP-Trap agarose (Chromotek) for 3h at 4°C ...
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bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
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bioRxiv - Biochemistry 2024Quote: Protein extracts containing GFP-tagged MIK2 or site-directed mutants were incubated with 20 µL of GFP-Trap beads (Chromotek) for 2 h with gentle mixing at 4 °C to immuno-precipitate receptor complexes ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells endogenously expressing C-terminal mEGFP-tagged MCM2 were transfected with plasmid (pCellCycleChromobody-RFP) containing RFP-PCNA chromobody (Chromotek, ccr) encoding single chain antibody recognizing endogenous PCNA protein ...
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bioRxiv - Cell Biology 2019Quote: The following antibodies were used for immunofluorescence: rat anti-RFP (Chromotek 1:1000); mouse anti-Dhc (Developmental Studies Hybridoma Bank ...
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bioRxiv - Pathology 2020Quote: ... The blot was further probed with anti-Myc rat monoclonal antibody (Chromotek, 9E1) in a 1:1,000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation of tagged PC1 was done using anti-GFP nanobodies coupled to magnetic agarose beads (GFP-trap, Chromotek cat. no. gtma-10) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... washed twice with ice-cold PBS and immunoprecipitation of GFP-tagged proteins was performed using GFP-Trap®_A kit (Chromotek, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
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bioRxiv - Plant Biology 2021Quote: ... The membranes were incubated with primary rat monoclonal anti-5F8 antibody (Chromotek, 1:1000) and secondary bovine anti-rat horseradish peroxidase (HRP)-conjugated (1:5000 ...
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bioRxiv - Plant Biology 2019Quote: ... RFP-fused proteins were detected using a rat anti-RFP antibody (1:5,000; ChromoTek). Light harvesting complex protein (LhcB1 ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves and pulldown assay were carried out according to102 with the following modifications: For immunoprecipitation of GFP-tagged proteins 50 µl of GFP-Trap magnetic beads (Chromotek, Planegg-Martinsried, Germany) were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™ ...
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bioRxiv - Plant Biology 2024Quote: ... Coimmunoprecipitation of GFP- or RFP-tagged proteins from total leaf protein extract by incubation with GFP- or RFP-Trap magnetic agarose beads (ChromoTek, Planegg-Martinsried Germany) was performed as previously reported (Azevedo et al. ...
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bioRxiv - Plant Biology 2024Quote: Co-immunoprecipitations of the GFP-tagged FtsZ2-1 line was performed in biological triplicates using GFP-Trap Magnetic Particles M-270 (Chromotek, Planegg-Martinsried, Germany). In brief ...
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bioRxiv - Molecular Biology 2021Quote: ... The following primary antibodies were used: Rat monoclonal anti-GFP antibody (Chromotek, 80626001AB, 1:5,000), Mouse monoclonal anti-tubulin anti-body (Merck ...