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Citations for ChromoTek :
101 - 150 of 197 citations for Recombinant Ovine IFNT protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Protein (1 mg/mL) was incubated with 25 μL of GFP-Trap magnetic micro beads (ChromoTek), or anti-FLAG M2 magnetic beads (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Protein extracts were then subjected to immunoprecipitation using agarose beads coupled with anti-RFP nanobodies (Chromotek). Eluates were collected and analysed by western blot ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated for 1 h with 25 μl GFP trap (ChromoTek; Planegg-Martinsried, Germany) on a rotating wheel at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... Total proteins were extracted and immunoprecipitation was performed with GFP-trap beads (Chromotek, Planegg-Martinsried, Germany). Proteins were stripped from the beads by boiling in 50 μl SDS loading buffer for 10 mins ...
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bioRxiv - Cell Biology 2022Quote: ... with equal amounts of total protein extract for each condition incubated with GFP-Trap_A beads (Chromotek) at 4°C for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... to equalise protein concentrations across the samples before immunoprecipitation with GFP-Trap resin (gta-20, ChromoTek) or anti-HA affinity matrix (11815016001 ...
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bioRxiv - Plant Biology 2022Quote: ... leaf material was harvested and protein extracts were subjected to immunoprecipitation using GFP-Trap beads (ChromoTek). 200 mg ground leaf material was subjected to protein extraction and immunoprecipitation using GFP-Trap beads (ChromoTek)19 ...
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bioRxiv - Plant Biology 2023Quote: ... GFP tagged proteins were detected using an anti-GFP antibody (1:4000; mAb rat 3H9; Chromotek) and a secondary anti-rat antibody (1:15000 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mg total proteins were incubated with 15 µl pre-equilibrated magnetic GFP-TRAP beads (Chromotek) for 2 h on a rotator at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek) and incubated for 2 hours at 4°C with gentle agitation ...
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bioRxiv - Biophysics 2019Quote: ... Presence of the mNeonGreen protein was probed using a monoclonal mNeonGreen antibody [32F6] (Chromotek; Planegg-Martinsried, Germany) at a 1:1000 dilution and visualized using a DyLight 680 secondary antibody (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... GFP-tagged proteins were immunoprecipitated by incubation with 25 µl of GFP-Trap magnetic agarose beads (Chromotek) overnight at 4 °C on a tube rotator ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extraction and immunoprecipitation were performed as described previously (Kadota et al., 2014) using GFP-trap (Chromotek) or GFP-clamp (Hansen et al. ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Plant Biology 2021Quote: ... The protein extracts were incubated with 20 μL of GFP-Trap magnetic agarose beads (GFP-TrapMA, Chromotek) for 2 h at 4 °C by vertical rotation (10 rpm) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were immunoprecipitated by incubation with anti-HA sepharose beads (Pierce) or anti-GFP-Trap beads (ChromoTek) for 2 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... To each 15 ml of protein extract 40 μl of GFP-TRAP AGAROSE BEADS (50 % slurry, ChromoTek) washed in extraction buffer were added and incubated with gentle agitation for 4 h at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... were proportioned for analysis of protein expression (5%) and immunoprecipitation (95%) using GFP-Trap beads (ChromoTek, Germany). Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Overexpressed protein was immunoprecipitated from the cell lysate using tag specific nano-trap (SPOT-Trap-MA, Chromotek) and analysed by WB.
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bioRxiv - Cell Biology 2021Quote: ... cleared lysates containing GFP-tagged proteins were added to anti-GFP agarose beads (GFP-Trap, ChromoTek, Germany) and incubated while rotating end over end for an hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... an equal amount of total protein extract for each condition was incubated with GFP-Trap_A beads (Chromotek) at 4°C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... Total proteins were inoculated with 30 μL GFP-Trap®_A beads (ChromoTek Inc., Hauppauge, NY, USA) at 4°C for 4h and then the bound proteins eluted by heating at 100°C for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... 200 μg of the extracted proteins were added to 25 μl of equilibrated magnetic agarose beads (Chromotek) and mixed in a rotating shaker for 45 min at 4 degrees ...
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bioRxiv - Plant Biology 2022Quote: ... 200 mg ground leaf material was subjected to protein extraction and immunoprecipitation using GFP-Trap beads (ChromoTek)19 ...
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bioRxiv - Molecular Biology 2023Quote: ... the GFP-fused substrate proteins were immunoprecipitated from the cell extract using GFP-Trap A beads (Chromotek) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg of total protein was incubated with 25 μl of GFP-Trap® Magnetic Beads (ChromoTek) in 1 ml of extraction buffer at 4 °C for 2 h in a rotating wheel ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Cell Biology 2020Quote: IPs with GFP-tagged proteins were performed with GFP-binding magnetic agarose beads (GFPtrap MA, Chromotek, #gtma-20). Per IP sample ...
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bioRxiv - Genetics 2020Quote: ... Four milligrams of total protein was mixed with 20 μl of GFP-conjugated agarose beads (#gta-20, Chromotek) equilibrated by washing in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2022Quote: ... Total proteins were then incubated with 30 μL GFP-Trap®_A beads (ChromoTek Inc., Hauppauge, NY, USA) at 4°C for 4h ...
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bioRxiv - Plant Biology 2023Quote: ... Five hundred microliters of the supernatant was incubated with 10µL of green fluorescent protein (GFP)-Trap A (Chromotek) bead slurry for 3 hours at 4°C with constant end-over-end rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Cleared supernatant containing roughly 5.4-6.6 mg of protein was mixed with 50 µl GFP-Trap-A agarose beads (Chromotek), which had been equilibrated in dilution buffer [10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble proteins were separated from the cell debris by centrifugation and incubated with GFP-Trap agarose beads (Chromotek) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 23225) and equal protein from each sample was loaded into pre-conditioned GFP-Trap A beads (gta-20; ChromoTek). Aliquots of the input sample(I ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were cleared by centrifugation (13000 g, 30 min) and the proteins were captured with GFP-trap (Chromotek), Myc-trap (Chromotek ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were then incubated for 1.5 h at 4 ⁰C with 25 µL RFP-Trap agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
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bioRxiv - Immunology 2023Quote: The coding sequence of the Dnmt1-chromobody targeting the human Dnmt1 protein [37] (Uniprot : P26358 · DNMT1_HUMAN) and lamin-chromobody (ChromoTek) targeting the lamin D0 from Drosophila monogaster (Uniprot ...
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bioRxiv - Plant Biology 2020Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... mCherry-Atg21 and mCherry-WIPI2d bait proteins are incubated at a concentration of 40 μM each with 10 μl RFP-Trap beads (Chromotek) in buffer containing 25 mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was measured using standard Bradford assay and 500 µg of total protein were incubated with GFP-Trap magnetic beads (ChromoTek) for 3h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: Protein extracts containing GFP-tagged EFR or site-directed mutants were incubated with 20 μL of GFP-Trap beads (Chromotek) or GFP-clamp beads (Hansen et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Myc-Trap agarose beads were used to pull down the Myc-TREM2 protein following the manufacturer’s instructions (Chromotek, yta-10). GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414 ...
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bioRxiv - Plant Biology 2021Quote: ... thaliana plants were incubated with 50-100 μl previously washed 50% GFP-binding protein beads slurry (Chromotek, GFP-Trap®) at 4 °C for 3 hours on a roller mixer ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested using the ‘on-bead digest protocol for mass spectrometry following immunoprecipitation with Nano-Traps’ recommended by Chromotek. In both cases mass spectra *.raw files were used to search the A ...
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bioRxiv - Plant Biology 2021Quote: ... 200 mg ground leaf material was subjected to protein extraction and immunoprecipitation using GFP-Trap beads (ChromoTek, IZB Martinsried, Germany) as previously described17.
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bioRxiv - Plant Biology 2020Quote: ... 50μg of total proteins were incubated 3H at 4°C with 30μl of GFP-Trap Agarose beads (Chromotek; gta-20) under gentle agitation for GFP-tagged protein purification ...