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Citations for ChromoTek :
201 - 224 of 224 citations for Recombinant Human CD33 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The Protein A agarose beads were pelleted by centrifugation and the supernatant was incubated with GFP-Trap® agarose beads (Chromotek) for 4 h at 4 °C on a mixing wheel ...
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bioRxiv - Neuroscience 2020Quote: ... GFP fusion proteins in fixed cells were labelled with Alexa Fluor 647-coupled GFP nanobodies (GFP-booster gb2AF647-50, Chromotek, Germany) at 1:100 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... and 500 µg of protein from each sample was incubated with 35 µL pre-washed GFP-Trap magnetic beads (Chromotek, Inc.) for 1 h at 4 °C with rotation (1 mg/mL final lysate concentration) ...
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bioRxiv - Plant Biology 2021Quote: One milligram of total protein extract was mixed with 25 μl of GFP Nanobody/VHH coupled to agarose beads (GFP-Trap® Agarose, Chromotek) and incubated for 3 hours and 30 minutes at 4°C with end to end rocking ...
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bioRxiv - Cell Biology 2022Quote: ... Then 800-1000 µg of total protein lysates were added to 25 μl of anti-GFP magnetic beads (ChromoTek GFP-Trap) and tumbled end-over-end for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: For CoIP experiments 500 μl protein extract was mixed with 25 μl calibrated anti-GFP magnetic beads (ChromoTek GFP-Trap®) and incubated on a rotator for 2 h ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Plant Biology 2020Quote: Blotted proteins were detected using anti-GFP (LifeTechnologies, Darmstadt, Germany) and anti-red FP (RFP) antibody (ChromoTek GmbH, Planegg-Martinsried, Germany) (both ...
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bioRxiv - Cell Biology 2023Quote: ... and approximately 250 µg of protein was incubated with anti-GFP-conjugated magnetic agarose beads (ChromoTek GFP-Trap® Magnetic Agarose) for 1-1.5 hours ...
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bioRxiv - Plant Biology 2024Quote: ... between EIN2-C and HAF2 following study previous briefly: Total protein extracts from plants mentioned in the paper were immunoprecipitated by DYKDDDDK Fab-Trap™ agarose beads (ChromoTek) for two hours at 4 °C with gentle rotating and then beads were washed five times with ice-cold 1XPBS buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Microbiology 2020Quote: ... Twenty milligram protein extracts were taken as input and the rest of the extracts were incubated with the GFP-Trap beads (ChromoTek, gta-20) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates (200 μg protein) from HEK-293T cells expressing WT-TRPC6-YFP was incubated with GFP M-trap beads (Chromotek GTD-20) to immunoprecipitate TRPC6 ...
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bioRxiv - Plant Biology 2019Quote: ... The protoplasts were collected and the subsequent work was operated following the protocol for immunoprecipitation of Myc-fusion proteins using Myc-Trap®_MA (Chromotek, Germany). Both the input and the IP samples were separated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of the protein lysates was incubated for 2 h with 10 µl of either Myc-Trap® or GFP-Trap® beads (Chromotek). To determine the dependence of interactions on RNA ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mL of protein extract was incubated with 60 μL (50% slurry) of GFP-trap magnetic agarose beads (ChromoTek, Planegg-Martinsried, Germany) for 3 h on a rotary shaker at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... One third of the whole cell lysate was pre-cleared with a 50:50 mixture of protein A/G magnetic beads and GFP-Trap (Chromotek, gtd-20) was pre-blocked with 1mg/mL ultra-pure BSA (AM2616 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 4 mgs of protein extract per sample was then incubated with GFP-Traps beads (ChromoTek; 3 μl/mg of extract) for 3 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Cell Biology 2019Quote: ... The cultures were harvested and total protein samples were extracted from vegetative mycelia and incubated with anti-GFP beads (GFP-Trap®_A, Chromotek, Hauppauge, NY, USA) 90 minutes at 4°C with gentle shaking following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... and the protein extract was separated on reducing SDS-PAGE and probed with commercial anti-RFP or anti-GFP antibodies (6G6, Chromotek or ab290, Abcam, respectively) to detect mCherry tagged MSBP1 or GFP-tagged ATI1 ...