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Citations for ChromoTek :
151 - 197 of 197 citations for Recombinant Canine CD274 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... equal concentration of total proteins was isolated and incubated with 20~30 μL of GFP-Trap agarose beads (ChromoTek, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining supernatants were used for immunoprecipitation of GFP-fused proteins agarose using GFP-Trap coupled to agarose beads (Chromotek) and following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted as indicated above and immunoprecipitation was performed with 15 μL of GFP-trap beads (ChromoTek, Germany) or ANTI-FLAG M2 Affinity Agarose Gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... Clover- and Cherry-tagged proteins were immunoprecipitated by incubation with either GFP-(gta) or RFP-Trap(rta) magnetic beads/agarose (Chromotek) for 1 h at 4°C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... the extract prepared from OSC lines expressing endogenously tagged proteins of interest was incubated with magnetic GFP-Trap agarose (Chromotek) for 3h at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and subjected to immunoprecipitation of RFP/mCherry/dsRed-tagged or GFP-tagged fusion proteins using RFP-trap or GFP-trap matrix (Chromotek), respectively ...
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bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mg of the total proteins were incubated with 20 μL of GFP-Trap magnetic agarose beads (GFP-TrapMA; Chromotek) for 2 hours at 4°C by vertical rotation ...
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bioRxiv - Plant Biology 2022Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins (GFP-Trap; ChromoTek GmbH, Germany; gta-20) was used for immunoprecipitation of mCerN1 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mL of the protein supernatant was incubated with 10 μL of magnetic GFP-Trap beads (Chromotek, Cat. No. gtma) or magnetic Myc-Trap beads (Chromotek ...
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bioRxiv - Developmental Biology 2023Quote: ... A maximum of 500 ug total protein in a volume of 950 ul was loaded per 25 ul GFP-Trap agarose beads (gta, Chromotek), prior washed with ice-cold PBS (2 min at 2’500 x g ...
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bioRxiv - Cell Biology 2024Quote: ... 0.6 mL of the resulting supernatant (containing 2 – 4 mg of proteins) were supplemented with 15 μL of RFP-Trap magnetic particles (ChromoTek), and incubated for 2.5 h at 4°C on a rotating wheel ...
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bioRxiv - Plant Biology 2024Quote: ... A volume of 2 mL of total protein was mixed with 25 μL of GFP-TRAP agarose beads (50% slurry, ChromoTek) and incubated shaking overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... To avoid unspecific protein binding the worm lysate was pre-cleared by incubation to uncoupled agarose beads (Chromotek, bab-20) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The insoluble material was removed by centrifugation and the lysates were subjected to immunoprecipitation either by subsequent incubations with anti-Ecad antibody SHE78-7 (4 μg/ml) and protein A-beads or by incubation with GFP-trap beads (Chromotek). After incubation ...
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bioRxiv - Biochemistry 2024Quote: Protein extracts containing GFP-tagged MIK2 or site-directed mutants were incubated with 20 µL of GFP-Trap beads (Chromotek) for 2 h with gentle mixing at 4 °C to immuno-precipitate receptor complexes ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were measured and 200 µg of lysates were subjected to immunoprecipitation with GFP-Trap®_MA magnetic beads (Chromotek) at 4° C for two hours on a rotating wheel ...
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bioRxiv - Microbiology 2019Quote: ... The Protein A agarose beads were pelleted by centrifugation and the supernatant was incubated with GFP-Trap® agarose beads (Chromotek) for 4 h at 4 °C on a mixing wheel ...
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bioRxiv - Neuroscience 2020Quote: ... GFP fusion proteins in fixed cells were labelled with Alexa Fluor 647-coupled GFP nanobodies (GFP-booster gb2AF647-50, Chromotek, Germany) at 1:100 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... and 500 µg of protein from each sample was incubated with 35 µL pre-washed GFP-Trap magnetic beads (Chromotek, Inc.) for 1 h at 4 °C with rotation (1 mg/mL final lysate concentration) ...
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bioRxiv - Plant Biology 2021Quote: One milligram of total protein extract was mixed with 25 μl of GFP Nanobody/VHH coupled to agarose beads (GFP-Trap® Agarose, Chromotek) and incubated for 3 hours and 30 minutes at 4°C with end to end rocking ...
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bioRxiv - Cell Biology 2022Quote: ... Then 800-1000 µg of total protein lysates were added to 25 μl of anti-GFP magnetic beads (ChromoTek GFP-Trap) and tumbled end-over-end for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: For CoIP experiments 500 μl protein extract was mixed with 25 μl calibrated anti-GFP magnetic beads (ChromoTek GFP-Trap®) and incubated on a rotator for 2 h ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Plant Biology 2020Quote: Blotted proteins were detected using anti-GFP (LifeTechnologies, Darmstadt, Germany) and anti-red FP (RFP) antibody (ChromoTek GmbH, Planegg-Martinsried, Germany) (both ...
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bioRxiv - Cell Biology 2023Quote: ... and approximately 250 µg of protein was incubated with anti-GFP-conjugated magnetic agarose beads (ChromoTek GFP-Trap® Magnetic Agarose) for 1-1.5 hours ...
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bioRxiv - Plant Biology 2024Quote: ... between EIN2-C and HAF2 following study previous briefly: Total protein extracts from plants mentioned in the paper were immunoprecipitated by DYKDDDDK Fab-Trap™ agarose beads (ChromoTek) for two hours at 4 °C with gentle rotating and then beads were washed five times with ice-cold 1XPBS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Microbiology 2020Quote: ... Twenty milligram protein extracts were taken as input and the rest of the extracts were incubated with the GFP-Trap beads (ChromoTek, gta-20) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates (200 μg protein) from HEK-293T cells expressing WT-TRPC6-YFP was incubated with GFP M-trap beads (Chromotek GTD-20) to immunoprecipitate TRPC6 ...
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bioRxiv - Plant Biology 2019Quote: ... The protoplasts were collected and the subsequent work was operated following the protocol for immunoprecipitation of Myc-fusion proteins using Myc-Trap®_MA (Chromotek, Germany). Both the input and the IP samples were separated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of the protein lysates was incubated for 2 h with 10 µl of either Myc-Trap® or GFP-Trap® beads (Chromotek). To determine the dependence of interactions on RNA ...
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bioRxiv - Microbiology 2019Quote: ... washed twice with ice-cold PBS and immunoprecipitation of GFP-tagged proteins was performed using GFP-Trap®_A kit (Chromotek, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mL of protein extract was incubated with 60 μL (50% slurry) of GFP-trap magnetic agarose beads (ChromoTek, Planegg-Martinsried, Germany) for 3 h on a rotary shaker at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... One third of the whole cell lysate was pre-cleared with a 50:50 mixture of protein A/G magnetic beads and GFP-Trap (Chromotek, gtd-20) was pre-blocked with 1mg/mL ultra-pure BSA (AM2616 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 4 mgs of protein extract per sample was then incubated with GFP-Traps beads (ChromoTek; 3 μl/mg of extract) for 3 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves and pulldown assay were carried out according to102 with the following modifications: For immunoprecipitation of GFP-tagged proteins 50 µl of GFP-Trap magnetic beads (Chromotek, Planegg-Martinsried, Germany) were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™ ...
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bioRxiv - Cell Biology 2019Quote: ... The cultures were harvested and total protein samples were extracted from vegetative mycelia and incubated with anti-GFP beads (GFP-Trap®_A, Chromotek, Hauppauge, NY, USA) 90 minutes at 4°C with gentle shaking following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... and the protein extract was separated on reducing SDS-PAGE and probed with commercial anti-RFP or anti-GFP antibodies (6G6, Chromotek or ab290, Abcam, respectively) to detect mCherry tagged MSBP1 or GFP-tagged ATI1 ...