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Citations for ChromoTek :
301 - 330 of 330 citations for Rat Mesoderm Specific Transcript Homolog Protein MEST ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Microbiology 2020Quote: ... Twenty milligram protein extracts were taken as input and the rest of the extracts were incubated with the GFP-Trap beads (ChromoTek, gta-20) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates (200 μg protein) from HEK-293T cells expressing WT-TRPC6-YFP was incubated with GFP M-trap beads (Chromotek GTD-20) to immunoprecipitate TRPC6 ...
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bioRxiv - Plant Biology 2019Quote: ... The protoplasts were collected and the subsequent work was operated following the protocol for immunoprecipitation of Myc-fusion proteins using Myc-Trap®_MA (Chromotek, Germany). Both the input and the IP samples were separated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of the protein lysates was incubated for 2 h with 10 µl of either Myc-Trap® or GFP-Trap® beads (Chromotek). To determine the dependence of interactions on RNA ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mL of protein extract was incubated with 60 μL (50% slurry) of GFP-trap magnetic agarose beads (ChromoTek, Planegg-Martinsried, Germany) for 3 h on a rotary shaker at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... One third of the whole cell lysate was pre-cleared with a 50:50 mixture of protein A/G magnetic beads and GFP-Trap (Chromotek, gtd-20) was pre-blocked with 1mg/mL ultra-pure BSA (AM2616 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... The GFP-CchA were enriched with a GFP-Trap kit (ChromoTek). After enrichment ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitation was carried out using the GFP-Trap Agarose KIT (ChromoTek), following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Cancer Biology 2023Quote: We used the PARP1-Trap Agarose kit [xtak-20] from Chromotek which consists of a PARP1 Nanobody/ VHH ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 4 mgs of protein extract per sample was then incubated with GFP-Traps beads (ChromoTek; 3 μl/mg of extract) for 3 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves and pulldown assay were carried out according to102 with the following modifications: For immunoprecipitation of GFP-tagged proteins 50 µl of GFP-Trap magnetic beads (Chromotek, Planegg-Martinsried, Germany) were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™ ...
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bioRxiv - Cell Biology 2019Quote: ... The cultures were harvested and total protein samples were extracted from vegetative mycelia and incubated with anti-GFP beads (GFP-Trap®_A, Chromotek, Hauppauge, NY, USA) 90 minutes at 4°C with gentle shaking following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... and the protein extract was separated on reducing SDS-PAGE and probed with commercial anti-RFP or anti-GFP antibodies (6G6, Chromotek or ab290, Abcam, respectively) to detect mCherry tagged MSBP1 or GFP-tagged ATI1 ...
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bioRxiv - Microbiology 2021Quote: ... GFP-EhPTEN1 was immunoprecipitated using GFP-Trap Agarose Kit (ChromoTek, Planegg, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Co-immunoprecipitation was performed using a GFP-Trap Kit (Chromotek, #gtma-20) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Immunoprecipitation was carried out using the GFP-trap® magnetic agarose kit (Chromotek, Germany) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Co-Immunoprecipitation (Co-IP) experiments were performed using the GFP-Trap®_M kit (ChromoTek). Proteins were extracted by grinding roots of 10-day-old Arabidopsis seedlings expressing pCAMV35S::GFP or pRXR1::RXR1-GFP in liquid N2 ...
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bioRxiv - Cell Biology 2022Quote: ... Co-immunoprecipitation was performed using a GFP- or RFP-Trap Kit (Chromotek, #gtma-20, #rtma-20) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Explant immunoprecipitation (IP) was then performed using a GFP-Trap Agarose Kit (ChromoTek, catalog number gtak-20) and proteins were eluted in 2X sample buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested two days later and ZAP was immunoprecipitated using GFP-Trap magnetic agarose kit (Chromotek) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The cultured explants were collected and immunoprecipitation (IP) was performed using GFP-Trap Agarose Kit (ChromoTek, cat# gtak-20). Immunoprecipitated proteins were eluted in 2X sample buffer ...
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bioRxiv - Molecular Biology 2021Quote: The SLO-treated trophozoite stage lysate of PfHDP-GFP transgenic parasites was immunoprecipitated using GFP-Trap®_A Kit (Chromotek)/ anti-GFP antibody following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Assay Kit (MilliporeSigma, Cat# 17-295) with adaptations based on the application notes on GFP- and RFP-Trap by ChromoTek. 100 Eye-antennal discs for each replicate were dissected in ice-cold 1xPBS ...
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bioRxiv - Plant Biology 2022Quote: Meiotic buds from ∼150 inflorescences from CDKD;3:YFP and wild type control were grinded in liquid nitrogen and processed using the ChromoTek GFP-Trap® Magnetic Agarose kit (Chromotek). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... Cell lysates were cleared and then incubated with GFP-Trap MA beads as instructed in ChromoTek GFP-Trap® MA immunoprecipitation kit (ChromoTek Inc., Hauppauge, NY). Cell lysates were subsequently washed twice in dilution buffer (10 mM Tris-HCl pH 7.5 ...