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Citations for ChromoTek :
101 - 150 of 304 citations for Rat Layilin LAYN Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: GFP-tagged target proteins and interacting proteins were affinity purified using GFP-trap agarose beads (Chromotek). Proteins were processed ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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bioRxiv - Plant Biology 2023Quote: ... GFP was detected using a rat 3H9 GFP-specific polyclonal antibody (diluted 1:1000)(ref 3H9-100ab, Chromotek, Germany ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal anti-CRM1/Exportin 1 (Novus, NB100-79802) and rat monoclonal anti-GFP (3H9) (ChromoTek, 3h9-100), rabbit polyclonal anti-LC3B (Novus biological ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined and an equal amount of protein was mixed with TurboGFP-Trap Magnetic Agarose (Chromotek) for 1 hour at RT with agitation ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were detected using α-HA (Chromotek) and α-GFP antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a 1:1,000 dilution of mouse anti-GFP [B-2] (SantaCruz Biotechnology) or a rat anti-RFP [5F8] (Chromotek) followed by a 1:5,000 dilution of the horseradish peroxidase-conjugated secondary antibody against mouse IgG (7076 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Another set of sections was incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... mCherry and GFP or EYFP signals in the neurons were enhanced by immunostainings using the following antibodies: rat anti-RFP (1:1,000, Chromotek) revealed by Cy3-conjugated donkey anti-rat secondary antibody (1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked as above and incubated in primary (rat anti-GFP, 1:200, ChromoTek, Cat. No. 3h9-100, clone 3H9) diluted in block as above ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was blocked using BlueBlock PF (SERVA) and developed using rat monoclonal anti-RFP 5F8 (1:2000, Chromotek) and rabbit anti-rat-peroxidase A9542 (1:20000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections ipsilateral to the injection side were incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... GFP fusion proteins were purified as follows: 200mg of protein extract was incubated with GFP-Trap Magnetic Agarose (ChromoTek) at 4°C for 20 minutes with gentle agitation ...
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bioRxiv - Plant Biology 2023Quote: GFP-tagged or RFP-tagged proteins were immunoprecipitated from total leaf protein extract by incubation with GFP-Trap or RFP-Trap magnetic agarose beads (ChromoTek). The Co-immunoprecipitation assay was carried out according to ChromoTek’s instructions whereby 300 μl of protein extract was diluted with 500 μl of ice-cold dilution buffer [10 mM Tris-HCl (pH 7.5),150 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Arabidopsis stable lines expressing native VAP27-1-RFP or Mito-mCherry were stained with anti-TraB1a mouse primary antibody at a dilution of 1:500 and anti-RFP rat primary antibody (Chromotek), followed by secondary antibody incubation with FITC conjugated against mouse ...
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bioRxiv - Developmental Biology 2021Quote: ... Lungs were collected at 5 days after tamoxifen injection and vibratome sections (350 µm) were stained with rat anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with primary antibody solution containing rabbit a-HA (1:500) (Cell Signalling) and rat a-RFP (1:500) (Chromotek) diluted in 3% BSA in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Neuroscience 2019Quote: ... and 100 ng/ml GFP-Trap Protein (ChromoTek). Tissue samples were homogenized three times at 250 rpm and ten times at 750 rpm on a variable-speed homogenizer (Glas-Col ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were purified by GFP-trap (ChromoTek) immunoprecipitation for 1 hour at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Protein complexes were immunoprecipitated using GFP-Trap (Chromotek) and washed 3 times with 50mM ammonium bicarbonate after the precipitation to remove detergent ...
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bioRxiv - Systems Biology 2023Quote: ... The proteins were immunoprecipitated using GFP-Trap (Chromotek) according to manufacturer’s protocol (with slight modifications) ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were incubated with GFP-Trap (ChromoTek) beads for 1h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... The following antibodies were used: rabbit or rat anti-dsRed (1:200, Clontech 632496 or 5F8 1:400, Chromotek 5f8-100); chicken anti-GFP (1:800 ...
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bioRxiv - Neuroscience 2024Quote: ... incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rat anti-RFP, 1:1000, 5f8, Chromotek; mouse anti-5mC ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Developmental Biology 2019Quote: ... Tagged proteins were immunoprecipitated with Myc-Trap_MA beads (ChromoTek) or anti-FLAG M2 Affinity gel (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... they were incubated with unlabelled (GFP-binding protein, Chromotek) and Abberior 635P conjugated Nanobody (GFP-booster ...
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bioRxiv - Cell Biology 2021Quote: ... protein lysates were added to RFP-Trap beads (Chromotek) and incubated for one hour at 4°C on a rotating shaker ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using anti-GFP antibody (3H9, Chromotek) or anti-alpha tubulin (T5168 ...
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Mutual repression between JNK/AP-1 and JAK/STAT stratifies cell behaviors during tissue regenerationbioRxiv - Developmental Biology 2022Quote: ... Membrane was incubated with primary antibodies in PBS-T (1% Triton-X in 1xPBS) - rat anti-GFP (Chromotek, 3H9-100, 1:1000) and mouse anti-α-tubulin (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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bioRxiv - Plant Biology 2024Quote: ... Coimmunoprecipitation of GFP- or RFP-tagged proteins from total leaf protein extract by incubation with GFP- or RFP-Trap magnetic agarose beads (ChromoTek, Planegg-Martinsried Germany) was performed as previously reported (Azevedo et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were immunoprecipitated with GFP-Trap beads (Chromotek, Germany), and analyzed by western blot with anti-GFP (Abiocode ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged proteins were affinity purified by GFP-trap (ChromoTek) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The protein complex pulled down by GFP-Trap beads (Chromotek) was washed three times with RIPA buffer and eluted in 30 μl of 1x Laemmli sample buffers by heating at 75°C for 10 min.
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bioRxiv - Cell Biology 2022Quote: ... GFP-GABARAP proteins were immunoprecipitated using GFP-TRAP beads (ChromoTek), incubating for 2hrs at 4°C ...
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bioRxiv - Microbiology 2023Quote: GFP-tagged proteins were immunoprecipitated using GFP-TRAP beads (Chromotek) using TNTE buffer (20mM Tris-HCL pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... or the 32F6 IgG2c monoclonal antibody against mNeonGreen protein (Chromotek). After several 5 min washes in PBS ...
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bioRxiv - Genetics 2023Quote: Protein complex was purified using GFP-Trap agarose beads (Chromotek) and separated by western blot ...
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bioRxiv - Plant Biology 2023Quote: ... protein extracts were incubated with GFP-Trap Agarose (Chromotek, Germany) at 4°C for 1 h with gentle rotation ...
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by GFP-Trap Agarose (Chromotek, gta).
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by RFP-Trap Agarose (Chromotek, rta). pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S: ...
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bioRxiv - Plant Biology 2024Quote: ... GFP-tagged proteins were detected by α-GFP antibodies (ChromoTek).
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: Immunoprecipitation of GFP-tagged proteins was performed using GFP-trap (Chromotek) according to the manufacturer ‘s instructions ...
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bioRxiv - Microbiology 2022Quote: ... mCherry fusion proteins were captured with RFP-Trap (Chromotek RTA-20) for 3 h at 4° C ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP fusion proteins were immunoprecipitated using GFP-Trap_MA (ChromoTek, Hauppauge, NY), recovered using a magnetic stand ...