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Citations for ChromoTek :
351 - 381 of 381 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labeled anti-GFP nanobodies (Chromotek) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labelled anti-GFP nanobodies (Chromotek) for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell lysates were subjected to WB and IP using the GFP-Trap® Agarose (sta-100, ChromoTek) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested two days later and ZAP was immunoprecipitated using GFP-Trap magnetic agarose kit (Chromotek) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... 2 mg of cell lysates were incubated with 25 ml of GFP-TRAP beads (Chromotek, GTA-20) for 1 hr at 4°C on rotation ...
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bioRxiv - Biochemistry 2022Quote: Soluble cell fractions were bound to 10 µL per mL of lysate of GFP-Trap® (ChromoTek™) agarose beads ...
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bioRxiv - Biochemistry 2023Quote: For creation of cells stably expressing plasmids containing EGFP-SNM1A or a Chromobody® of RFP-PCNA (Chromotek), ∼5 x 105 cells were seeded in wells of a 6-well plate and immediately transfected with the desired plasmid (2.6 µg ...
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bioRxiv - Cell Biology 2024Quote: ... Equal volumes of clarified and normalised cell lysates were incubated with 15-20 μl GFP-Trap Agarose (ChromoTek) for overnight at 4 °C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... EGFP-INPP5E or its mutants were then immunoprecipitated from 1 mg of cell lysate by overnight rotation at 4°C with GFP-Trap_MA beads (Chromotek). Beads were then washed thrice in buffer containing 50mM Tris-HCl pH=7.5 and 150mM NaCl buffer (no protease inhibitors or detergent) ...
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bioRxiv - Cancer Biology 2019Quote: ... Pre-clearing of cell lysates was done by adding 50 µl pre-equilibrated binding control magnetic agarose beads (Chromotek) and incubated for 30 min at 4°C on a rotating platform ...
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bioRxiv - Microbiology 2020Quote: ... incubated O/N in primary antibody (Rabbit Pan-ADPr 1:1000, Cell Signaling E6F6A; Rabbit GFP 1:1000, Chromotek PABG1-100 ...
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bioRxiv - Cell Biology 2022Quote: ... 2mg equivalent of cell lysate was incubated with 30 µl of GFP-Trap agarose beads (Chromotek, ACT-CM-GFA0050) at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2019Quote: Soluble fractions of cell extracts were immunoprecipitated with an anti-GFP antibody coupled to magnetic beads (GFP-Trap_MA, ChromoTek) or an anti-HA antibody coupled to agarose beads (EZview Red Anti-HA Affinity Gel ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed for Nanobody Trap experiments following the manufacturer’s protocol (Chromotek #gta-20, #gtma-20, or #v5tma-20). The bound proteins were eluted by boiling in 2X Western sample buffer and subjected to SDS-PAGE (immunoprecipitated IP fraction) ...
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bioRxiv - Molecular Biology 2023Quote: Preparation of DNase-digested cell extracts and immunoprecipitation was done using GFP-Trap® Magnetic Particles M-270 (Chromotek) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 100 µg for the input were saved and then cell extracts were immunoprecipitated by a GFP-Trap (Chromotek, Germany). As a negative control for pull-downs assays the eGFP plasmid (Clontech Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant plasmid DNA was sequenced and transfected into Baby Hamster Kidney fibroblasts (BHK-1 cells) that were modified to contain concatemeric E.coli Lactose operator (Lac O) sequences inserted into a single locus (Chromotek, Germany).
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bioRxiv - Biochemistry 2020Quote: ... Both these cell pellet-derived samples and the culture supernatant samples were incubated with 10 μl RFP-trap MA (Chromotek) for one hour at 24 °C with constant mixing ...
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bioRxiv - Cell Biology 2020Quote: ... Cell debris was pelleted at 17,900 rcf for 15 min and the supernatant was bound to α-myc beads (Chromotek) for 4 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear and cytoplasmic proteins were isolated from glioblastoma cells harboring ASH2L-GFP and used for GFP-affinity purification with GFP-Trap agarose beads (#gta-200, Chromotek). Purified proteins were on-bead digested and tryptic peptides were analyzed by nanoflow-LC-MS/MS with a high performance nanoflow-HPLC Orbitrap based ms/ms system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... were purified from three confluent 10-cm dishes of transfected U2OS cells following the above protocol for denaturing immunoprecipitation using Myc-trap beads (ChromoTek). A control purification from cells transfected with empty vector alone was performed in parallel ...
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bioRxiv - Cell Biology 2023Quote: ... U2-OS KI eGFP-DEK or MCF10A cells were transfected with a PCNA-specific chromobody expression vector (pCCC-TagRFP, Chromotek) using Effectene (Qiagen U2-OS KI eGFP-DEK ...
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bioRxiv - Cell Biology 2024Quote: Samples for STED microscopy were prepared by incubating yeGFP-tagged Cdc3 cells (wild-type CDC11 or mutants) with a 1/250 dilution of an anti-GFP nanobody Atto 647N (ChromoTek), as previously detailed in (52) ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: F2H cells (genetically modified baby hamster kidney (BHK) fibroblasts) were cultured according to the manufacturer’s instructions (ChromoTek GmbH, Planegg-Martinsried, Germany). For detection of protein-protein interactions in live cells ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells endogenously expressing C-terminal mEGFP-tagged MCM2 were transfected with plasmid (pCellCycleChromobody-RFP) containing RFP-PCNA chromobody (Chromotek, ccr) encoding single chain antibody recognizing endogenous PCNA protein ...
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bioRxiv - Pathology 2020Quote: ... aliquots of the supernatants equivalent to 2 x 108 cells were incubated with 10 μL anti-Myc agarose beads (Myc-Trap™, Chromotek) for 1 h at 4 °C ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... EGFP-Tat and EGFP were immunoprecipitated from U2OS cells using 25 μl of GFP-Trap magnetic beads (ChromoTek GmbH, Planegg-Martinsried, Germany) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... cells were lysed as described above and the same buffers used as above except using GFP-trap® affinity beads (ChromoTek™) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... One plate (∼5x107 cells) per condition was harvested using RIPA lysis and immunoperciptated with GFP-trap agarose beads (ChromoTek ref# gta- 10) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were lysed with ice-cold NP-40 lysis buffer (see above) and cell lysates were incubated with GFP-Trap Magnetic Beads (ChromoTek, Planegg-Martinsried, Germany) end-over-end rocking for 2 hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed and treated for immunofluorescence with α-SINV C antibody at 1:200 dilution and GFP-Booster_Atto488 (ChromoTek GmbH, #gba488-100) at 1:500 dilution following the procedure described in (Garcia-Moreno et al. ...