Labshake search
Citations for ChromoTek :
351 - 400 of 420 citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 Surface Lipoprotein P27 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: eYFP-tagged fusion proteins were immunoprecipitated using anti-GFP magnetic agarose beads (Chromotek, gtma-20). After induction of gene expression of lentiviral transduced KO2 cells with 1 µg/ml doxycycline ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-RFP (Invitrogen, #R10367, 1:1000 for WB and Chromotek #5F8, 1:1000 for WB), anti-phospho myosin light chain (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2022Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: Affinity purification was performed using anti-GFP nanobody coupled magnetic beads (∼ 30µl slurry) (Chromotek AB_2631358). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... The precleared lysate was incubated with an anti-GFP-Trap-A beads (ChromoTek, gta-20) antibody overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... the RFP-Booster Alexa Fluor 568 (alpaca monoclonal anti-RFP Nanobody; 1:1000; rb2AF568, ChromoTek) was used with second antibodies to amplify the signal of NLS-sfCherry.
-
bioRxiv - Microbiology 2020Quote: ... GFP-Trap Magnetic Agarose beads or control beads without antibodies were used according to the manufactured protocol (both Chromotek, Planegg-Martinsried, Germany). The loaded magnetic beads were heated for 10 min at 95 °C in SDS loading buffer for the dissociation of purified proteins ...
-
bioRxiv - Microbiology 2019Quote: ... The nanoboosters - single-domain alpaca antibody fragments covalently coupled to fluorescent dyes — GFP-Booster Atto488 and RFP-Booster Atto594 (Chromotek, Martinsried, Germany) — were used to boost eYFP and mCherry fluorescence ...
-
bioRxiv - Microbiology 2021Quote: ... Protein extracts were then subjected to immunoprecipitation using agarose beads coupled with anti-RFP nanobodies (Chromotek). Eluates were collected and analysed by western blot ...
-
bioRxiv - Plant Biology 2021Quote: ... Anti-GFP coated beads were added (25 μL ChromoTek GFP-Trap or 50 μL MACS magnetic) and IP was carried out as per the manufacturers’ instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... each supernatant was incubated with 50 µL of Anti-cMyc agarose beads (Chromotek, 004282-07-02) at 4°C for 4 h ...
-
bioRxiv - Plant Biology 2023Quote: ... The supernatant was subjected to immunoprecipitation for 1 hour using anti-GFP (ChromoTek, Planegg- Martinsried Germany), anti-RFP (ChromoTek ...
-
bioRxiv - Plant Biology 2023Quote: ... 600 µL of plant lysate was incubated with 30 µL of anti-mRFP magnetic beads (Chromotek) in a rotary mixer for 1 hrs at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... The rest of the supernatant was mixed with 8 µL anti-GFP beads (Chromotek, gta-20). The mixture was incubated for 2 h at 4 °C while rotating ...
-
bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
-
bioRxiv - Microbiology 2021Quote: ... cells were fixed and treated for immunofluorescence with α-SINV C antibody at 1:200 dilution and GFP-Booster_Atto488 (ChromoTek GmbH, #gba488-100) at 1:500 dilution following the procedure described in (Garcia-Moreno et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
-
bioRxiv - Microbiology 2024Quote: ... Secondary antibody incubation was carried out for 1 hour at room temperature with GFP-Booster Alexa Fluor 488 (Chromotek, gb2AF488, 1:500 dilution) and Alexa Fluor 647 donkey anti-rabbit IgG (ThermoFisher ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labeled anti-GFP nanobodies (Chromotek) for 30 min ...
-
bioRxiv - Plant Biology 2022Quote: ... Twenty μl of anti-GFP beads was mixed with 1.4 ml of filtered extracts (Chromotek, Munich, Germany) and incubated at 4□ for 2 hours ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labelled anti-GFP nanobodies (Chromotek) for 30 min ...
-
bioRxiv - Cell Biology 2021Quote: ... cleared lysates containing GFP-tagged proteins were added to anti-GFP agarose beads (GFP-Trap, ChromoTek, Germany) and incubated while rotating end over end for an hour at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: Expressed GFP-ALDH9A1 was enriched using anti-GFP beads following the manufacturer’s protocol for GFP-Trap (Chromotek). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: ... then incubated overnight on a Nutator with anti-GFP bound agarose beads (Chromotek GFP-Trap® #gta). Lysates and beads were separated in a centrifuge ...
-
bioRxiv - Cell Biology 2023Quote: ... The signal of mCherry and GFP was enhanced using a rat mAb anti-RFP (Chromotek, 5f8-100) and a rabbit pAb anti-GFP antibody (BIOZOL/MBL ...
-
bioRxiv - Plant Biology 2019Quote: ... (2009) using for BPC1-RFP an anti-RFP VHH coupled to magnetic agarose beads RFP-trap_MA® (Chromotek). Real-time PCR assays were performed to determine the enrichment of the fragments ...
-
bioRxiv - Biochemistry 2021Quote: ... immunoprecipitation was performed using either GFP-Trap (high affinity anti-GFP alpaca nanobody bound to agarose beads; Chromotek) or anti-HA beads (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2019Quote: ... diluted with 300 µl buffer and loaded on magnetic agarose anti-GFP beads (GFP-trap®_MA, ChromoTek) for 1 h at 4°C ...
-
bioRxiv - Immunology 2023Quote: The input and elution samples obtained after immunoprecipitation using anti-GFP beads (Miltenyi or agarose beads from Chromotek). If necessary ...
-
bioRxiv - Cell Biology 2020Quote: ... Co-IP’s on the total cell lysates or on subcellular fractions (nuclear and cytoplasmic) were performed at different timepoints using anti-GFP VHH coupled to magnetic agarose beads (GFP-Trap_MA, Chromotek) or ...
-
bioRxiv - Plant Biology 2019Quote: ... The homogenate was then centrifuged twice at 10,000 rpm for 10 min and incubated with 20 ul of anti-mCherry Trap agarose beads (Chromotek) for 12-16 h at 4°C on a rocking shaker ...
-
bioRxiv - Neuroscience 2020Quote: ... 2 and 3 GAGs fusion proteins were first concentrated by immunoprecipitation of roughly 20,000 nematodes per genotype using Trap-A bead coupled with anti-mNG nanobody (Chromotek). 1.5 U of heparanase I and III (Sigma ...
-
bioRxiv - Cancer Biology 2021Quote: ... The precleared lysate was incubated with 30 μl of magnetic agarose beads coupled to anti-GFP nanobodies (GFPTrap; ChromoTek) for 1 hr at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: Single-molecule tracking was carried out in modified Tyrode’s solution supplement with 0.8% BSA and ATTO647N-conjugated anti-GFP nanobodies (1:15000 dilution, GFPBooster-ATTO647N, Chromotek, #gba647n) on Nanoimager microscope (Oxford Nanoimaging ...
-
bioRxiv - Developmental Biology 2021Quote: ... blocked as above and incubated in primary (rat anti-GFP, 1:200, ChromoTek, Cat. No. 3h9-100, clone 3H9) diluted in block as above ...
-
bioRxiv - Plant Biology 2021Quote: ... The membrane was blocked using BlueBlock PF (SERVA) and developed using rat monoclonal anti-RFP 5F8 (1:2000, Chromotek) and rabbit anti-rat-peroxidase A9542 (1:20000 ...
-
bioRxiv - Plant Biology 2022Quote: ... The ribosylated NbFBN1a2A-GFP and NbFBN1a-GFP were detected by the anti-pan-ADP-ribose binding reagent after they were pulled down with Co-IP by GFP-Trap_A (ChromoTek).
-
bioRxiv - Microbiology 2020Quote: ... and then incubated with 25 μl anti-HA magnetic beads (Pierce) or with 25 μL GFP-Trap Magnetic Agarose (Chromotek) overnight at 4 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
-
bioRxiv - Plant Biology 2020Quote: ... Small cuts from the infiltrated regions were incubated for one hour in anti-Spot-Tag VHH/Nanobodies conjugated to the organic fluorophore ATTO488 (Chromotek), diluted 1:1000 in 10 mM Tris-HCl solution pH 7.5 ...
-
bioRxiv - Plant Biology 2020Quote: ... The roots were incubated overnight at 4°C with the secondary anti-mouse (1:500; Jackson immunoreactive, conjugated to Rhodamine Red) and eba488-10 Spot Label ATTO488 (1:1000, Chromotek) diluted in PBS supplemented with 2% (w/v ...
-
bioRxiv - Molecular Biology 2020Quote: ... then mixtures were centrifuged (15 min, 4º, 14000 rcf) and the supernatant (input) was added to washed anti-GFP beads (Chromotek). Beads and lysates were incubated 2-4 h at 4 °C while rotating ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek, Germany, Cat. No. gtma-20) for 3h at 4°C while rotating ...
-
bioRxiv - Cancer Biology 2019Quote: ... The following nanobodies and dyes were used for whole body immunostaining: Atto647N conjugated anti-RFP/mCherry nanobooster (Chromotek, rba647n-100), Atto594 conjugated anti-RFP/mCherry nanobooster (Chromotek ...
-
bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
-
bioRxiv - Developmental Biology 2021Quote: ... Vibratome sections prepared from inflated and fixed lobes at 350 µm thickness were stained with chicken anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lungs were collected at 5 days after tamoxifen injection and vibratome sections (350 µm) were stained with rat anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
-
bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was then aspirated without disturbing the upper lipid layer and split by half into either the anti-GFP agarose beads or the control blocked beads (22 μl, Chromotek). After head over tail rotation at 4°C for 90 min ...
-
bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...