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Citations for ChromoTek :
1 - 50 of 347 citations for RNA Binding Motif Protein 45 RBM45 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2019Quote: ... or control binding antibody (bmab-20, Chromotek). For tight chromatin fractionation ...
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bioRxiv - Biophysics 2020Quote: ... they were incubated with unlabelled (GFP-binding protein, Chromotek) and Abberior 635P conjugated Nanobody (GFP-booster ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Cell Biology 2020Quote: IPs with GFP-tagged proteins were performed with GFP-binding magnetic agarose beads (GFPtrap MA, Chromotek, #gtma-20). Per IP sample ...
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bioRxiv - Cell Biology 2021Quote: ... and binding control agarose beads (Chromotek).
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bioRxiv - Microbiology 2019Quote: ... binding control magnetic agarose beads (Chromotek) were used ...
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bioRxiv - Plant Biology 2021Quote: ... thaliana plants were incubated with 50-100 μl previously washed 50% GFP-binding protein beads slurry (Chromotek, GFP-Trap®) at 4 °C for 3 hours on a roller mixer ...
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bioRxiv - Developmental Biology 2024Quote: ... To avoid unspecific protein binding the worm lysate was pre-cleared by incubation to uncoupled agarose beads (Chromotek, bab-20) for 1 hour at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... or binding control beads (Chromotek #bmab-20). The immune complexes were run on SDS-PAGE and analyzed by Western blotting.
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bioRxiv - Cell Biology 2022Quote: ... 10 µl of GFP-binding agarose beads (Chromotek) were incubated with the protein extract for 1 hour at 4 °C in head-over-shoulder rotation ...
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bioRxiv - Plant Biology 2024Quote: ... a negative control (binding control agarose beads, Chromotek) and input control were used ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using anti-GFP antibody (3H9, Chromotek) or anti-alpha tubulin (T5168 ...
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bioRxiv - Molecular Biology 2021Quote: ... CFTR was immunoprecipitated as described 45 using GFP-Trap Agarose (Chromotek).
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bioRxiv - Cell Biology 2023Quote: ... or the 32F6 IgG2c monoclonal antibody against mNeonGreen protein (Chromotek). After several 5 min washes in PBS ...
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bioRxiv - Plant Biology 2024Quote: ... GFP-tagged proteins were detected by α-GFP antibodies (ChromoTek).
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bioRxiv - Microbiology 2021Quote: ... 25 μl of binding control agarose beads (Chromotek; bab20), prepared in dilution buffer (10 mM Tris-Cl pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... or with binding control agarose beads (Chromotek #bab-20) using RIPA lysis buffer and according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was precleared with binding control agarose beads (ChromoTek) for 1hr at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... the protein–chromatin solution was incubated with GFP antibody-conjugated magnetic beads (ChromoTek) under 4°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... total proteins were incubated with 10 μl agarose conjugated anti-GFP antibodies (Chromotek) for 3 h and washed 3 times with the washing buffer (50 mM HEPES [pH 7.5] ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Cell Biology 2021Quote: ... the proteins were detected by Western blot analyses with the indicated antibodies (GFP (Chromotek) 1:4,000 ...
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bioRxiv - Plant Biology 2019Quote: ... RFP-fused proteins were detected using a rat anti-RFP antibody (1:5,000; ChromoTek). Light harvesting complex protein (LhcB1 ...
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bioRxiv - Plant Biology 2019Quote: ... mCherry proteins were detected by a rat anti-RFP antibody (5F8-100, 1:2,000, Chromotek), and then with a goat anti-rat IgG HRP-conjugated secondary antibody (AP202P ...
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bioRxiv - Plant Biology 2023Quote: ... GFP tagged proteins were detected using an anti-GFP antibody (1:4000; mAb rat 3H9; Chromotek) and a secondary anti-rat antibody (1:15000 ...
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bioRxiv - Biochemistry 2020Quote: ... Diluted lysate was pre-cleared by incubation with binding control agarose beads (bab-20, CHROMOTEK), at a volume of slurry equivalent to 1:200 (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated for 1 hr with 20 μl of agarose binding control beads (ChromoTek), equilibrated in IP buffer 2 (200 mM NaCl ...
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bioRxiv - Biophysics 2019Quote: ... Presence of the mNeonGreen protein was probed using a monoclonal mNeonGreen antibody [32F6] (Chromotek; Planegg-Martinsried, Germany) at a 1:1000 dilution and visualized using a DyLight 680 secondary antibody (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 30 μl GFP-Trap Agarose or Binding Control Agarose Beads (Chromotek, gta-10 and bab-20) were washed once in dilution buffer (10mM tricHCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was precleared in 20 μl binding control magnetic beads (bmp-20, ChromoTek; Munich, DE) for 30 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 2 in CTD domain rat 3E10 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 5 in CTD domain rat 3E8 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Genetics 2023Quote: ... Each sample was incubated for preclearing with 200 μL slurry of binding control magnetic agarose beads (ChromoTek bmab) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The insoluble material was removed by centrifugation and the lysates were subjected to immunoprecipitation either by subsequent incubations with anti-Ecad antibody SHE78-7 (4 μg/ml) and protein A-beads or by incubation with GFP-trap beads (Chromotek). After incubation ...
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bioRxiv - Cancer Biology 2019Quote: ... Pre-clearing of cell lysates was done by adding 50 µl pre-equilibrated binding control magnetic agarose beads (Chromotek) and incubated for 30 min at 4°C on a rotating platform ...
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bioRxiv - Plant Biology 2022Quote: ... The ribosylated NbFBN1a2A-GFP and NbFBN1a-GFP were detected by the anti-pan-ADP-ribose binding reagent after they were pulled down with Co-IP by GFP-Trap_A (ChromoTek).
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: Blotted proteins were detected using anti-GFP (LifeTechnologies, Darmstadt, Germany) and anti-red FP (RFP) antibody (ChromoTek GmbH, Planegg-Martinsried, Germany) (both ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-KAT7 was incubated with either HIS6-LphD or GST-RomA (HIS6-MBP or GST-MLY1 as binding controls) and the pulldown was performed using DYKDDDDK Fab-Trap beads (Chromotek) followed by western blot analysis.
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bioRxiv - Neuroscience 2023Quote: Chromatin samples were thawed on ice and pre-cleared for 15 minutes by rotation in 25 µl of pre-washed binding control magnetic agarose beads (Chromotek). Chromatin was diluted ten-fold in sonication buffer without SDS ...
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bioRxiv - Cell Biology 2021Quote: ... or to affinity purify ribosomes with translated RNA using GFP-TRAP beads (magnetic agarose beads coated with Alpaca anti-GFP VHH antibody, Chromotek). Incubation with the GFP-TRAP beads proceeded for 3 h at 4°C with gentle end-over-end mixing in a tube rotator and was followed by three washes with high salt buffer (350 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Plant Biology 2020Quote: ... and the protein extract was separated on reducing SDS-PAGE and probed with commercial anti-RFP or anti-GFP antibodies (6G6, Chromotek or ab290, Abcam, respectively) to detect mCherry tagged MSBP1 or GFP-tagged ATI1 ...
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bioRxiv - Microbiology 2022Quote: GFP-tagged target proteins and interacting proteins were affinity purified using GFP-trap agarose beads (Chromotek). Proteins were processed ...