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Citations for ChromoTek :
101 - 150 of 175 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Diluted lysate was pre-cleared by incubation with binding control agarose beads (bab-20, CHROMOTEK), at a volume of slurry equivalent to 1:200 (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... The cleared lysate was incubated with an equivalent of 7 μl GFP-trap slurry (Chromotek) for 6 h at 4°C on a rotor ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates from cells expressing GFP tagged proteins were immunoprecipitated using a GFP-Trap Kit (Chromotek) as per manufacturer’s instructions and immunoblotting performed as above ...
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bioRxiv - Cell Biology 2020Quote: ... Precleared lysates were incubated for 2.5h at RT with pre-washed GFP-traps® (Chromotek) and subsequently subjected to stringent washes in denaturing conditions (8 M urea ...
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bioRxiv - Cell Biology 2021Quote: ... clarified lysates were supplemented with GFP Trap Magnetic Agarose (gtma-20, Chromotek, Planegg-Martinsried, Germany) and incubated with rotation for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Precleared lysates were incubated with GFP-trap or RFP-trap agarose beads (Chromotek, Munich, Germany) for 2 hours at 4°C with mild rotation ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were precleared and the pulldown was performed using GFP-trap magnetic agarose beads (Chromotek), following the manufacturer’s instructions at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining diluted lysate was then mixed with 10 μl GFP-Trap agarose beads (Chromotek) that were pre-equilibrated with GFP-Trap dilution buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 200 g for lysate was added to 25 µL of HaloTrap beads (Chromotek, #ota-10), prewashed with 500 µL of 10 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysates were then incubated with 10 μl equilibrated GFP-TRAP beads/sample (gtma, Chromotek) for 60 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated for 1 hr with 20 μl of agarose binding control beads (ChromoTek), equilibrated in IP buffer 2 (200 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... The precleared lysate was incubated with an anti-GFP-Trap-A beads (ChromoTek, gta-20) antibody overnight ...
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bioRxiv - Microbiology 2019Quote: ... Immunoprecipitations from total cell lysates were performed by addition of 25 µL GFP-Trap resin (Chromotek) and inversion at 4°C for 2 hrs ...
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bioRxiv - Cell Biology 2019Quote: ... immunoprecipitation was performed by incubating the lysates at 40C for 1hour with RFP-trap beads (Chromotek). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... Lysates were cleared by 10 min centrifugation at 15000xg and incubated with GFP-trap beads (Chromotek). The material bound on the beads was eluted in Laemmli buffer and the proteins were separated by SDS-PAGE using Criterion 4-15% acrylamide gels (BioRad ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were cleared by centrifugation (20,000x g, 20 min) and incubated with GFP-Trap beads (ChromoTek) for 3 hr at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Diluted cell lysates were used for an IP with GFP-Trap magnetic agarose beads from Chromotek. The GFP-Trap beads were equilibrated by washing 25 µl of beads slurry with 500 µl ice-cold dilution buffer and subsequently centrifuged for 2 min at 2,500 g ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoprecipitation was performed by incubating the lysates with GFP-Trap Magnetic beads (Chromotek cat: gtd-10) for 1.5 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were pre equilibrated with dilution buffer and incubated with GFP Trap beads (gta-10, Chromotek) on a rotating wheel at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... This diluted lysate was then incubated with equilibrated GFP-Trap Magnetic beads (Chromotek, Cat#gtmak, RRID:AB_2631358) for 45 minutes-1 hour at 4°C with agitation ...
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bioRxiv - Plant Biology 2023Quote: ... 600 µL of plant lysate was incubated with 30 µL of anti-mRFP magnetic beads (Chromotek) in a rotary mixer for 1 hrs at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... VirE2-Venus and Venus cell lysates were incubated with GFP-Trap magnetic agarose beads (Chromotek, USA) at 4°C for 2 hr with gentle shaking ...
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bioRxiv - Immunology 2019Quote: ... For immunoprecipitation of EGFP-RIPK2 equal amounts of cell lysates were incubated with GFP-Trap_A beads (Chromotek) and washed three times with lysis buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... Then pre-cleared lysate was incubated with 70 µl pre-equilibrated GFP-Trap magnetic agarose beads (Chromotek) overnight at 4°C on a rotating platform ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amounts of lysate (1 mg) were then immunoprecipitated with mNeongreen-trap magnetic agarose resin (Chromotek, ntma) for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Overexpressed protein was immunoprecipitated from the cell lysate using tag specific nano-trap (SPOT-Trap-MA, Chromotek) and analysed by WB.
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared and used for immunoprecipitation with 15 μl GFP-trap or Myc-trap (Chromotek). Beads were washed with lysis buffer and bound material was eluted from beads with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation and mixed with GFP-Trap Magnetic Agarose beads (Chromotek, cat# gtma-10) or protein G Dynabeads (Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell lysates were subjected to WB and IP using the GFP-Trap® Agarose (sta-100, ChromoTek) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cleared lysates containing GFP-tagged proteins were added to anti-GFP agarose beads (GFP-Trap, ChromoTek, Germany) and incubated while rotating end over end for an hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... clarified lysate was incubated with 25 µl GFP-Trap magnetic beads (ChromoTek GFP-Trap® Magnetic Agarose), at 4°C for 2 h ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were cleared by centrifugation (6,000 g, 30 min, 4°C) and incubated with GFP-Trap (Chromotek) for 2h ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were diluted in washing buffer and then incubated with washed GFP-Trap® Agarose beads (Chromotek) for 1h30 ...
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bioRxiv - Immunology 2023Quote: ... 2 mg of cell lysates were incubated with 25 ml of GFP-TRAP beads (Chromotek, GTA-20) for 1 hr at 4°C on rotation ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates incubated on a nutator at 4°C for two hours with 20μl of GFP-Trap beads (ChromoTek). GFP-Trap complexes were then washed four times in IP buffer and re-suspended in 2× SDS-PAGE sample buffer ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were incubated for 2 hours at 4°C with 25 μL GFP-Trap coupled agarose beads (Chromotek). After incubation ...
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bioRxiv - Neuroscience 2019Quote: ... roughly equal volumes of sample and control lysate were incubated with 100 µl GFP-Trap MA (ChromoTek gtma), Myc-Trap MA (ChromoTek ytma) ...
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bioRxiv - Cell Biology 2019Quote: ... BTLA–mGFP and PD-1–mGFP were co-immunoprecipitated from the lysate using GFP-Trap (Chromotek, #gta-20). Equal fractions of the immunoprecipitates were subjected to SDS-PAGE and indicated antibodies.
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bioRxiv - Molecular Biology 2021Quote: ... Lysate was cleared by centrifugation and incubated for 2h at 4°C with magnetic GFP-Trap agarose (Chromotek). The beads were washed 3 x 10 min with Buffer 3 and were either used for mass spectrometry analysis or the proteins were eluted in 1× SDS buffer with 5 min incubation at 95°C for western blotting ...
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bioRxiv - Biochemistry 2022Quote: Soluble cell fractions were bound to 10 µL per mL of lysate of GFP-Trap® (ChromoTek™) agarose beads ...
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bioRxiv - Cell Biology 2024Quote: Equal volumes of the cleared and normalised lysates were incubated with 15-20 μl GFP-Trap Agarose (ChromoTek) pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Equal volumes of clarified and normalised cell lysates were incubated with 15-20 μl GFP-Trap Agarose (ChromoTek) for overnight at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... Equal volumes of the cleared and normalised lysates were incubated with 15-20 μl GFP-Trap Agarose (ChromoTek) pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was then added to 25 µl of GFP-Trap_MA magnetic agarose beads (#gtma-20, ChromoTek, Germany) prewashed twice in 1 ml wash buffer ...
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bioRxiv - Cell Biology 2022Quote: ... EGFP-INPP5E or its mutants were then immunoprecipitated from 1 mg of cell lysate by overnight rotation at 4°C with GFP-Trap_MA beads (Chromotek). Beads were then washed thrice in buffer containing 50mM Tris-HCl pH=7.5 and 150mM NaCl buffer (no protease inhibitors or detergent) ...
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bioRxiv - Cancer Biology 2019Quote: ... Pre-clearing of cell lysates was done by adding 50 µl pre-equilibrated binding control magnetic agarose beads (Chromotek) and incubated for 30 min at 4°C on a rotating platform ...
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bioRxiv - Cancer Biology 2021Quote: ... The precleared lysate was incubated with 30 μl of magnetic agarose beads coupled to anti-GFP nanobodies (GFPTrap; ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of lysate was incubated with 25 μL of equilibrated GFP-trap magnetic agarose beads (Chromotek, gtma-20) for 1 h with end-over-end rotation at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 2mg equivalent of cell lysate was incubated with 30 µl of GFP-Trap agarose beads (Chromotek, ACT-CM-GFA0050) at 4°C for 2 hours ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were cleared by centrifugation (13000 g, 30 min) and the proteins were captured with GFP-trap (Chromotek), Myc-trap (Chromotek ...