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Citations for ChromoTek :
101 - 150 of 151 citations for PGA3 PGA4 PGA5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibody (mouse anti-mNeonGreen [32F6], Chromotek, 1:250) diluted in PBT + 1x WBR ...
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bioRxiv - Biophysics 2019Quote: ... Presence of the mNeonGreen protein was probed using a monoclonal mNeonGreen antibody [32F6] (Chromotek; Planegg-Martinsried, Germany) at a 1:1000 dilution and visualized using a DyLight 680 secondary antibody (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 2 in CTD domain rat 3E10 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 5 in CTD domain rat 3E8 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 15 µl of Myc-Trap Agarose (with anti-Myc alpaca antibody) (Chromotek, cat. No. yta) and incubated for 3 h at 4°C with end-over-end rotation ...
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bioRxiv - Cell Biology 2019Quote: ... and the soluble fraction was incubated with single chain anti GFP antibody (GFP-binder) (GFP-Trap-M; Chromotek) conjugated to sepharose beads ...
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bioRxiv - Cell Biology 2019Quote: ... GFP antibody (GFPTrap; gta-20) and control agarose beads (bab-20) used for immunoprecipitation were purchased from ChromoTek, Germany ...
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bioRxiv - Plant Biology 2021Quote: ... For the localization of RNAPII we used antibodies recognizing RNAPII phosphorylated at serine 5 (Chromotek, diluted 1:200) and serine 2 (Chromotek ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Further primary antibodies used for western blots in this manuscript are as follows: monomeric NeonGreen (1:1’000, Chromotek), phospho-eIF2α (1:1’000 ...
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bioRxiv - Plant Biology 2023Quote: ... GFP was detected using a rat 3H9 GFP-specific polyclonal antibody (diluted 1:1000)(ref 3H9-100ab, Chromotek, Germany ...
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bioRxiv - Biophysics 2023Quote: ... The membrane was air-dried for 1 h and immunoblotted with a primary antibody against mNeonGreen tag (Chromotek) at room temperature for 1 h followed by a secondary antibody (680nm anti-mouse ...
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bioRxiv - Microbiology 2020Quote: ... incubated O/N in primary antibody (Rabbit Pan-ADPr 1:1000, Cell Signaling E6F6A; Rabbit GFP 1:1000, Chromotek PABG1-100 ...
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bioRxiv - Physiology 2022Quote: ... 2mg/ml was used per IP experiments performed with camelid antibodies GFP-Trap Magnetic Agarose (Cat# gtma-20, Chromotek); RFP-Trap Magnetic Agarose (Cat# rtma-20 ...
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bioRxiv - Neuroscience 2022Quote: ... Another set of sections was incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... mCherry and GFP or EYFP signals in the neurons were enhanced by immunostainings using the following antibodies: rat anti-RFP (1:1,000, Chromotek) revealed by Cy3-conjugated donkey anti-rat secondary antibody (1:500 ...
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bioRxiv - Cell Biology 2019Quote: Soluble fractions of cell extracts were immunoprecipitated with an anti-GFP antibody coupled to magnetic beads (GFP-Trap_MA, ChromoTek) or an anti-HA antibody coupled to agarose beads (EZview Red Anti-HA Affinity Gel ...
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bioRxiv - Neuroscience 2022Quote: ... Sections ipsilateral to the injection side were incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at +4°C with the primary antibody (rabbit anti-GFP, 1:600, Chromotek) diluted in the blocking solution ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was probed with the indicated primary antibody (rabbit anti-GFP [1:1500, Abcam, #ab290], mouse anti-RFP [1:1000, Chromotek, #6G6] ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was transferred to a fresh tube and incubated with anti-GFP antibody coupled with magnetic agarose (GFP-Trap, Chromotek) overnight at 4 °C on a rocking platform ...
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bioRxiv - Molecular Biology 2021Quote: ... They were then incubated with antibody-bead conjugate (Anti-FLAG® M2 Magnetic Beads, Millipore & Sigma or GFP-Trap_A, Chromotek) overnight at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The blot was incubated overnight on a shaker (4°C) with the primary antibody (anti-GFP 3H9, anti-RFP 6G6 (Chromotek) or anti-tRFP (Evrogen)) ...
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bioRxiv - Genetics 2021Quote: ... Pulldowns of the GFP-fusion proteins were performed by incubating tissue homogenate (7-day-old seedlings) or cell lysate (CHO cell lysate) with agarose beads that conjugated with GFP antibodies (GFP Nano-Trap, Chromotek). After incubations of 4-24 hours with gentle inverting ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... or to affinity purify ribosomes with translated RNA using GFP-TRAP beads (magnetic agarose beads coated with Alpaca anti-GFP VHH antibody, Chromotek). Incubation with the GFP-TRAP beads proceeded for 3 h at 4°C with gentle end-over-end mixing in a tube rotator and was followed by three washes with high salt buffer (350 mM KCl ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with primary antibody solution containing rabbit a-HA (1:500) (Cell Signalling) and rat a-RFP (1:500) (Chromotek) diluted in 3% BSA in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... portions of the blot were reacted against anti- mNeonGreen (mouse monoclonal antibody 32F6 from Chromotek, Inc., at 1:200 dilution), anti- HA (rabbit monoclonal antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... The CUT and TAG experiment was done for two biological replicates using antibodies anti-GFP (Chromotek, PABG1-100, 1:50), anti-YFP (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on 8% SDS-PAGE gels and detected by western blot using monoclonal mouse anti-HA (MMS-101R; Covance) or monoclonal mouse anti-RFP antibodies (6G6; ChromoTek) prior to secondary antibody treatment with polyclonal goat anti-mouse conjugated to horseradish peroxidase (115–035-146 ...
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bioRxiv - Cell Biology 2024Quote: ... The insoluble material was removed by centrifugation and the lysates were subjected to immunoprecipitation either by subsequent incubations with anti-Ecad antibody SHE78-7 (4 μg/ml) and protein A-beads or by incubation with GFP-trap beads (Chromotek). After incubation ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Slides were subsequently blocked overnight in PBS containing 3% BSA before labelling with anti-mNeonGreen mouse monoclonal antibody (32F6; 1:300; Chromotek, Germany) followed by Alexa Fluor 488 goat anti-mouse antibody (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: Blotted proteins were detected using anti-GFP (LifeTechnologies, Darmstadt, Germany) and anti-red FP (RFP) antibody (ChromoTek GmbH, Planegg-Martinsried, Germany) (both ...
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bioRxiv - Developmental Biology 2023Quote: ... The following antibodies were used: rabbit or rat anti-dsRed (1:200, Clontech 632496 or 5F8 1:400, Chromotek 5f8-100); chicken anti-GFP (1:800 ...
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bioRxiv - Neuroscience 2024Quote: ... incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rat anti-RFP, 1:1000, 5f8, Chromotek; mouse anti-5mC ...
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bioRxiv - Microbiology 2020Quote: ... GFP-Trap Magnetic Agarose beads or control beads without antibodies were used according to the manufactured protocol (both Chromotek, Planegg-Martinsried, Germany). The loaded magnetic beads were heated for 10 min at 95 °C in SDS loading buffer for the dissociation of purified proteins ...
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bioRxiv - Plant Biology 2021Quote: ... For immunoprecipitations, α-rabbit Trueblot agarose beads (eBioscience) coupled with α-FLS2 antibodies (Chinchilla et al., 2007) or GFP-Trap agarose beads (ChromoTek) were used and incubated with the crude extract for 3-4 h at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The nanoboosters - single-domain alpaca antibody fragments covalently coupled to fluorescent dyes — GFP-Booster Atto488 and RFP-Booster Atto594 (Chromotek, Martinsried, Germany) — were used to boost eYFP and mCherry fluorescence ...
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Mutual repression between JNK/AP-1 and JAK/STAT stratifies cell behaviors during tissue regenerationbioRxiv - Developmental Biology 2022Quote: ... Membrane was incubated with primary antibodies in PBS-T (1% Triton-X in 1xPBS) - rat anti-GFP (Chromotek, 3H9-100, 1:1000) and mouse anti-α-tubulin (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed and treated for immunofluorescence with α-SINV C antibody at 1:200 dilution and GFP-Booster_Atto488 (ChromoTek GmbH, #gba488-100) at 1:500 dilution following the procedure described in (Garcia-Moreno et al. ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Cell Biology 2024Quote: ... thawed 90-nm-thick cryosections were incubated for 30 min at RT with a rabbit anti-GFP antibody (Chromotek, PABG1, 1:200 dilution) followed by protein A conjugated to 10 nm gold particles for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibody incubation was carried out for 1 hour at room temperature with GFP-Booster Alexa Fluor 488 (Chromotek, gb2AF488, 1:500 dilution) and Alexa Fluor 647 donkey anti-rabbit IgG (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then probed overnight at 4°C with TBST buffer containing 1% non-fat dry milk and primary antibodies: mouse monoclonal anti-RFP (Chromotek, clone 6G6, 1:3,000), rabbit polyclonal anti-GFP (SYSY ...