Labshake search
Citations for ChromoTek :
301 - 350 of 363 citations for Mouse Anti Human Papilloma virus type 11 711 13 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... then incubated overnight on a Nutator with anti-GFP bound agarose beads (Chromotek GFP-Trap® #gta). Lysates and beads were separated in a centrifuge ...
-
bioRxiv - Cell Biology 2023Quote: ... The signal of mCherry and GFP was enhanced using a rat mAb anti-RFP (Chromotek, 5f8-100) and a rabbit pAb anti-GFP antibody (BIOZOL/MBL ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 15 µl of Myc-Trap Agarose (with anti-Myc alpaca antibody) (Chromotek, cat. No. yta) and incubated for 3 h at 4°C with end-over-end rotation ...
-
bioRxiv - Plant Biology 2019Quote: ... (2009) using for BPC1-RFP an anti-RFP VHH coupled to magnetic agarose beads RFP-trap_MA® (Chromotek). Real-time PCR assays were performed to determine the enrichment of the fragments ...
-
bioRxiv - Biochemistry 2021Quote: ... immunoprecipitation was performed using either GFP-Trap (high affinity anti-GFP alpaca nanobody bound to agarose beads; Chromotek) or anti-HA beads (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2019Quote: ... diluted with 300 µl buffer and loaded on magnetic agarose anti-GFP beads (GFP-trap®_MA, ChromoTek) for 1 h at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... and the soluble fraction was incubated with single chain anti GFP antibody (GFP-binder) (GFP-Trap-M; Chromotek) conjugated to sepharose beads ...
-
bioRxiv - Immunology 2023Quote: The input and elution samples obtained after immunoprecipitation using anti-GFP beads (Miltenyi or agarose beads from Chromotek). If necessary ...
-
bioRxiv - Cell Biology 2020Quote: ... Co-IP’s on the total cell lysates or on subcellular fractions (nuclear and cytoplasmic) were performed at different timepoints using anti-GFP VHH coupled to magnetic agarose beads (GFP-Trap_MA, Chromotek) or ...
-
bioRxiv - Plant Biology 2019Quote: ... The homogenate was then centrifuged twice at 10,000 rpm for 10 min and incubated with 20 ul of anti-mCherry Trap agarose beads (Chromotek) for 12-16 h at 4°C on a rocking shaker ...
-
bioRxiv - Neuroscience 2020Quote: ... 2 and 3 GAGs fusion proteins were first concentrated by immunoprecipitation of roughly 20,000 nematodes per genotype using Trap-A bead coupled with anti-mNG nanobody (Chromotek). 1.5 U of heparanase I and III (Sigma ...
-
bioRxiv - Cancer Biology 2021Quote: ... The precleared lysate was incubated with 30 μl of magnetic agarose beads coupled to anti-GFP nanobodies (GFPTrap; ChromoTek) for 1 hr at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... mCherry and GFP or EYFP signals in the neurons were enhanced by immunostainings using the following antibodies: rat anti-RFP (1:1,000, Chromotek) revealed by Cy3-conjugated donkey anti-rat secondary antibody (1:500 ...
-
bioRxiv - Cell Biology 2019Quote: Soluble fractions of cell extracts were immunoprecipitated with an anti-GFP antibody coupled to magnetic beads (GFP-Trap_MA, ChromoTek) or an anti-HA antibody coupled to agarose beads (EZview Red Anti-HA Affinity Gel ...
-
bioRxiv - Neuroscience 2021Quote: Single-molecule tracking was carried out in modified Tyrode’s solution supplement with 0.8% BSA and ATTO647N-conjugated anti-GFP nanobodies (1:15000 dilution, GFPBooster-ATTO647N, Chromotek, #gba647n) on Nanoimager microscope (Oxford Nanoimaging ...
-
bioRxiv - Developmental Biology 2021Quote: ... blocked as above and incubated in primary (rat anti-GFP, 1:200, ChromoTek, Cat. No. 3h9-100, clone 3H9) diluted in block as above ...
-
bioRxiv - Plant Biology 2021Quote: ... The membrane was blocked using BlueBlock PF (SERVA) and developed using rat monoclonal anti-RFP 5F8 (1:2000, Chromotek) and rabbit anti-rat-peroxidase A9542 (1:20000 ...
-
bioRxiv - Plant Biology 2022Quote: ... The ribosylated NbFBN1a2A-GFP and NbFBN1a-GFP were detected by the anti-pan-ADP-ribose binding reagent after they were pulled down with Co-IP by GFP-Trap_A (ChromoTek).
-
bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at +4°C with the primary antibody (rabbit anti-GFP, 1:600, Chromotek) diluted in the blocking solution ...
-
bioRxiv - Microbiology 2020Quote: ... and then incubated with 25 μl anti-HA magnetic beads (Pierce) or with 25 μL GFP-Trap Magnetic Agarose (Chromotek) overnight at 4 °C ...
-
bioRxiv - Genetics 2020Quote: ... The supernatant was transferred to a fresh tube and incubated with anti-GFP antibody coupled with magnetic agarose (GFP-Trap, Chromotek) overnight at 4 °C on a rocking platform ...
-
bioRxiv - Molecular Biology 2021Quote: ... They were then incubated with antibody-bead conjugate (Anti-FLAG® M2 Magnetic Beads, Millipore & Sigma or GFP-Trap_A, Chromotek) overnight at 4 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
-
bioRxiv - Plant Biology 2020Quote: ... Small cuts from the infiltrated regions were incubated for one hour in anti-Spot-Tag VHH/Nanobodies conjugated to the organic fluorophore ATTO488 (Chromotek), diluted 1:1000 in 10 mM Tris-HCl solution pH 7.5 ...
-
bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... then mixtures were centrifuged (15 min, 4º, 14000 rcf) and the supernatant (input) was added to washed anti-GFP beads (Chromotek). Beads and lysates were incubated 2-4 h at 4 °C while rotating ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek, Germany, Cat. No. gtma-20) for 3h at 4°C while rotating ...
-
bioRxiv - Cancer Biology 2019Quote: ... The following nanobodies and dyes were used for whole body immunostaining: Atto647N conjugated anti-RFP/mCherry nanobooster (Chromotek, rba647n-100), Atto594 conjugated anti-RFP/mCherry nanobooster (Chromotek ...
-
bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
-
bioRxiv - Developmental Biology 2021Quote: ... Vibratome sections prepared from inflated and fixed lobes at 350 µm thickness were stained with chicken anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lungs were collected at 5 days after tamoxifen injection and vibratome sections (350 µm) were stained with rat anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
-
bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... or to affinity purify ribosomes with translated RNA using GFP-TRAP beads (magnetic agarose beads coated with Alpaca anti-GFP VHH antibody, Chromotek). Incubation with the GFP-TRAP beads proceeded for 3 h at 4°C with gentle end-over-end mixing in a tube rotator and was followed by three washes with high salt buffer (350 mM KCl ...
-
bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was then aspirated without disturbing the upper lipid layer and split by half into either the anti-GFP agarose beads or the control blocked beads (22 μl, Chromotek). After head over tail rotation at 4°C for 90 min ...
-
bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
-
bioRxiv - Microbiology 2022Quote: ... the supernatants were incubated with a 5-μL slurry of anti-Spot nanobody-coupled magnetic agarose beads (Spot-Trap, ChromoTek) for 3 h at 4 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... Anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins (GFP-Trap; ChromoTek GmbH, Germany; gta-20) was used for immunoprecipitation of mCerN1 ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were lysed with a buffer containing 1% Triton X-100 for 20 min and the lysate was incubated with anti-GFP magnetic beads (Chromotek) for 2 hours in 0.33% Triton X-100 solution ...
-
bioRxiv - Developmental Biology 2023Quote: ... The CUT and TAG experiment was done for two biological replicates using antibodies anti-GFP (Chromotek, PABG1-100, 1:50), anti-YFP (Merck Millipore ...
-
bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
-
bioRxiv - Microbiology 2024Quote: ... and then incubated with 25 μl of anti-HA magnetic beads (Pierce) or with 25 μl of GFP-Trap Magnetic Agarose (ChromoTek) overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
-
bioRxiv - Cell Biology 2024Quote: ... The insoluble material was removed by centrifugation and the lysates were subjected to immunoprecipitation either by subsequent incubations with anti-Ecad antibody SHE78-7 (4 μg/ml) and protein A-beads or by incubation with GFP-trap beads (Chromotek). After incubation ...
-
bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then transferred to new collection tubes and incubated with to 30 µl of prewashed anti-myc or -V5 beads (Chromotek) and equilibrated to a final volume of 1000 µl by adding lysis buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... transduced cells were incubated with 100 μL Hybridization Buffer containing 2 μL 12.5 μM CF568-labled gRNA FISH probes and 1:2000 AF647-labeled anti-GFP nanobody (Chromotek, gb2AF647-50) for 4 hours in the dark ...
-
bioRxiv - Cell Biology 2022Quote: ... Then 800-1000 µg of total protein lysates were added to 25 μl of anti-GFP magnetic beads (ChromoTek GFP-Trap) and tumbled end-over-end for 1 h ...
-
bioRxiv - Developmental Biology 2020Quote: For CoIP experiments 500 μl protein extract was mixed with 25 μl calibrated anti-GFP magnetic beads (ChromoTek GFP-Trap®) and incubated on a rotator for 2 h ...
-
bioRxiv - Pathology 2020Quote: ... aliquots of the supernatants equivalent to 2 x 108 cells were incubated with 10 μL anti-Myc agarose beads (Myc-Trap™, Chromotek) for 1 h at 4 °C ...
-
bioRxiv - Genetics 2023Quote: ... for 30 min to eliminate the background signal, and then incubated with anti-HP1α (NB110-40623) (Novus, St Louis, MO, USA) or GFP-Trap (gta-20) (Chromotek, Germany) antibodies at 4 °C overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... and approximately 250 µg of protein was incubated with anti-GFP-conjugated magnetic agarose beads (ChromoTek GFP-Trap® Magnetic Agarose) for 1-1.5 hours ...