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Citations for ChromoTek :
1 - 50 of 90 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: YFP-ATM was immunoprecipitated from stably transfected HEK293 cells by GFP-TRAP (Chromotek). The immunoprecipitates were suspended in kinase assay buffer containing 2 μCi of [γ-32P] ATP ...
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bioRxiv - Plant Biology 2023Quote: ... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
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bioRxiv - Immunology 2023Quote: The coding sequence of the Dnmt1-chromobody targeting the human Dnmt1 protein [37] (Uniprot : P26358 · DNMT1_HUMAN) and lamin-chromobody (ChromoTek) targeting the lamin D0 from Drosophila monogaster (Uniprot ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: RPE-1 cells were transfected with 10µg Cell Cycle-Chromobody® plasmid (TagRFP) (From Chromotek, Planegg, Germany) using JET PRIME kit (Polyplus Transfection ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with RFP antibody (Chromotek) labelled with NHS AF647 for 15 min and subsequently imaged in TIRF microscopy ...
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bioRxiv - Genetics 2021Quote: ... Pulldowns of the GFP-fusion proteins were performed by incubating tissue homogenate (7-day-old seedlings) or cell lysate (CHO cell lysate) with agarose beads that conjugated with GFP antibodies (GFP Nano-Trap, Chromotek). After incubations of 4-24 hours with gentle inverting ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed using X1 RIPA buffer (ChromoTek) containing protease and phosphatase inhibitors (100X ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysis and coimmunoprecipitation using GFP-Trap (Chromotek) was performed as described above ...
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bioRxiv - Microbiology 2019Quote: ... Pellets where then ressuspended in cell lysis buffer (Chromotek) supplemented with phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed using GFP-Trap_A Lysis buffer (Chromotek), 0.25 % Deoxycolate ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysate was incubated with GFP-Trap beads (Chromotek) for 1 hour and washed 3 x 1ml with lysis buffer and resuspended in SDS-PAGE loading buffer.
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bioRxiv - Plant Biology 2023Quote: ... cell lysates were incubated with GFP-trap beads (ChromoTek) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lysates were added to GFP-Trap agarose beads (ChromoTek) pre-equilibrated with lysis buffer and incubated for 2hrs at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... cells were transiently transfected with PARP1 chromobody tagged-RFP (Chromotek), according to QIAGEN Effectene protocol and imaged 24 h after transfection.
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with GFP-TRAP_A or M (Chromotek) bead slurry for 1 to 16h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... COS7 cells were transfected and immunoprecipitated using GFP-TRAP (ChromoTek), as described previously (Kedersha et al. ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysate was incubated with equilibrated GFP-Trap resin (Chromotek) at 4°C for 2 h ...
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bioRxiv - Immunology 2024Quote: ... equal cell numbers were also lysed in Lysis Buffer (Chromotek) with Protease Inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with GFP-booster (Alexa Fluor488, Chromotek, 1:200) at the secondary antibody step ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysate was then incubated with equilibrated GFP-Trap resin (Chromotek) at 4°C for 2 h ...
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bioRxiv - Immunology 2022Quote: ... supernatants from cell lysates were processed with an RFP-trap (Chromotek) or with Dynabeads protein A/protein G (Thermofisher ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysates were incubated with GFP-Trap® Dynabeads™ (Chromotek) at 4°C for 1 hour while rotated ...
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bioRxiv - Cell Biology 2021Quote: ... the cell lysate was incubated with GFP-Trap Sepharose beads (ChromoTek) for 3 h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed before incubation with GFP-Trap Agarose beads (Chromotek) overnight at 4 °C using manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... After removing cell debris by centrifugation 10ul GFP-trap Sepharose (Chromotek) slurry was added to the lysate and incubation was carried out for 1.5 hours on a rotating shaker at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then sequentially incubated in mouse anti-mNeonGreen (IgG2c; Chromotek), followed by rat anti-mouse IgG(H+L)-AlexaFluor488 (ThermoFisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were subjected to immunoprecipitation with GFP-trap dynabeads (Chromotek gtdk-20) following the manufacturers’ protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with RFP-Booster nanobody coupled to Atto 594 (Chromotek) at a dilution of 1:200.
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bioRxiv - Cell Biology 2019Quote: ... Clearfield cell lysate was IPed using GFP-Trap-A beads from Chromotek then the blotted with purified serum ...
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bioRxiv - Biochemistry 2023Quote: ... IGF2BP1 was immunoprecipitated from HEK293T cells by GFP-trap magnetic beads (ChromoTek). The cells were lysed using cold lysis buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... clarified cell lysates were incubated with GTP-trap agarose beads (ChromoTek GmbH, Germany) for two hours before washing with GFP wash buffer (10 mM Tris [pH 7.5] ...
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bioRxiv - Molecular Biology 2019Quote: ... The total cell lysate was used for immunoprecipitation with GFP-Trap magnetic beads (ChromoTek) for 2h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared cell lysates were incubated with 25 μl GFP-Trap agarose resin slurry (ChromoTek) for 1 h to immunoprecipitate proteasomes ...
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bioRxiv - Biochemistry 2021Quote: ... the whole-cell lysate was incubated with pre-equilibrated GFP-Trap Dynabeads (gtd, Chromotek) for binding ...
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bioRxiv - Microbiology 2020Quote: Cells were transfected with plasmids and lysed for immunoprecipitation using GFP-Trap beads (Chromotek), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... anti-myc beads (Cell signalling-9B11) and anti-GFP (GFP-Trap Magnetic Agarose-Chromotek) were used.
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bioRxiv - Cell Biology 2020Quote: ... 300 µg of cell lysate was used for GFP-Trap with 20 µl beads (Chromotek) (50% slurry) ...
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bioRxiv - Biochemistry 2020Quote: ... the cell lysates were incubated with the GFP-Trap reagent (Cat. number gta-100, ChromoTek) for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed before incubated with GFP-Trap Agarose beads overnight at 4 °C (Chromotek) and washed as manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates from cells expressing GFP tagged proteins were immunoprecipitated using a GFP-Trap Kit (Chromotek) as per manufacturer’s instructions and immunoblotting performed as above ...
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bioRxiv - Biochemistry 2022Quote: ... precleared cell extracts (10,000, 5 min) and incubated the supernatants with Myc-trap resin (Chromotek) for 3 hours at 4 °C.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were pelleted and incubated with 10 µl of pre-equilibrated GFP-Trap_M beads (Chromotek) 2 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... MYC-tagged protein was purified from transduced cells (see above) with Myc-trap agarose (Chromotek), a nanobody based system ...
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bioRxiv - Microbiology 2019Quote: ... Immunoprecipitations from total cell lysates were performed by addition of 25 µL GFP-Trap resin (Chromotek) and inversion at 4°C for 2 hrs ...
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bioRxiv - Cancer Biology 2019Quote: Cells from an 80% confluent 10-cm plate were harvested and the immunoprecipitation protocol from Chromotek was followed ...
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bioRxiv - Biochemistry 2022Quote: ... Diluted cell lysates were used for an IP with GFP-Trap magnetic agarose beads from Chromotek. The GFP-Trap beads were equilibrated by washing 25 µl of beads slurry with 500 µl ice-cold dilution buffer and subsequently centrifuged for 2 min at 2,500 g ...
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bioRxiv - Plant Biology 2024Quote: ... VirE2-Venus and Venus cell lysates were incubated with GFP-Trap magnetic agarose beads (Chromotek, USA) at 4°C for 2 hr with gentle shaking ...
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bioRxiv - Immunology 2019Quote: ... For immunoprecipitation of EGFP-RIPK2 equal amounts of cell lysates were incubated with GFP-Trap_A beads (Chromotek) and washed three times with lysis buffer ...