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Citations for ChromoTek :
201 - 250 of 304 citations for Human Single stranded DNA binding protein 4 SSBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... GFP-trap beads (GFPTrap-A kit, Chromotek) were equilibrated with dilution buffer (same as the extraction buffer except for the omission of Triton X-100) ...
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bioRxiv - Biochemistry 2021Quote: ... samples were spun down 10 min at 14000 rpm and 4°C and supernatant was added to GFP-Trap agarose beads from Chromotek and incubated for 1.5 hours at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were collected (centrifugation for 10 min at 21,100 g and 4 °C) and the supernatant was added to 25 µl of blocked GFP-Trap Agarose (gta, ChromoTek).
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bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 40 μl of GFP-Trap MA magnetic Beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
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bioRxiv - Plant Biology 2020Quote: ... The blot was incubated overnight on a shaker (4°C) with the primary antibody (anti-GFP 3H9, anti-RFP 6G6 (Chromotek) or anti-tRFP (Evrogen)) ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was incubated for 1 h at 4 °C with GFP-Selector beads (NanoTag) (Fig. 4A, B and D) or with Myc-trap beads (Chromotek) (Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 15 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 150 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were cleared by 10 min centrifugation at 12,000 rpm and the supernatant was incubated on a rotor wheel at 4°C for 3 h with 25 µl of GFP-Trap magnetic agarose bead slurry (Chromotek), pre-washed with extraction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... on a rotating wheel at 4° C for 1 h in triplicates with GFP-Trap agarose beads (#gta-200, Chromotek) or control agarose beads (Chromotek) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were cleared by centrifugation for 30 min at 4°C and incubated with GFP-trap magnetic agarose beads (Chromotek) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatant was cleared at 14000 rpm for 10 minutes and incubated for 30 minutes rotating at 4 °C together with 25 µl pre-equilibrated Spot-Trap beads (Chromotek). Beads were washed 4 times with GFP PD buffer at 2500 g for 2 minutes at 4 ° C ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 4°C and added to GFP-Trap Magnetic agarose beads (Chromotek, cat #gtma-10) for 1 hr at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged for 20 min (full speed, 4°C) and the supernatant was incubated with mEGFP-Trap Agarose beads (ChromoTek) for 1 hour according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mL of supernatant was incubated at 4°C for 2 h with GFP-Trap_A beads (Chromotek, Planegg-Martinsried, Germany). The beads were collected by centrifugation at 800 × g and washed four times in 1 mL washing buffer (25 mM Tris pH=7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysate was centrifuged at 17,000 g for 10 min at 4 °C and supernatant was applied to 20 μl of GFP-Trap beads (Chromotek) and shaken for 2 hr at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... Four milligrams of total protein was mixed with 20 μl of GFP-conjugated agarose beads (#gta-20, Chromotek) equilibrated by washing in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2022Quote: ... Total proteins were then incubated with 30 μL GFP-Trap®_A beads (ChromoTek Inc., Hauppauge, NY, USA) at 4°C for 4h ...
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bioRxiv - Plant Biology 2023Quote: ... Five hundred microliters of the supernatant was incubated with 10µL of green fluorescent protein (GFP)-Trap A (Chromotek) bead slurry for 3 hours at 4°C with constant end-over-end rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Cleared supernatant containing roughly 5.4-6.6 mg of protein was mixed with 50 µl GFP-Trap-A agarose beads (Chromotek), which had been equilibrated in dilution buffer [10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... immunoprecipitation was performed using GFP-Trap Kit (Chromotek) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... an mNeonGreen-Trap Agarose Kit (ntak-20; Chromotek) to pull down Tld1-mNeonGreen (mNG ...
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bioRxiv - Microbiology 2019Quote: ... and 200 ng total membrane proteins were incubated for 1 h at 4°C with gentle shaking on a roller mix with either 25 µl GFP-Trap® agarose beads (Chromotek) in a final volume of 100 µl 1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... The rest of the supernatants were incubated for 15 min at 4°C with 10 μl of GFP-Trap® Agarose beads (gta-10; Chromotek) to remove free GFP and spun at 1200 xg for 1 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Cytoplasmic and nuclear fractions were incubated for 30 minutes at 4°C under agitation with 120 µL of control agarose beads (ChromoTek, Germany). Pre-cleared lysates were collected by a centrifuging 2 min at 400g (4°C) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting protein extracts were centrifuged at maximum speed on a table-top centrifuge for 15 minutes at 4 °C and the supernatant (soluble fraction) incubated with 25 μl GFP-trap magnetic beads (ChromoTek, gtma) for four hours at 4 °C under constant rotation ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble proteins were separated from the cell debris by centrifugation and incubated with GFP-Trap agarose beads (Chromotek) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 23225) and equal protein from each sample was loaded into pre-conditioned GFP-Trap A beads (gta-20; ChromoTek). Aliquots of the input sample(I ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were cleared by centrifugation (13000 g, 30 min) and the proteins were captured with GFP-trap (Chromotek), Myc-trap (Chromotek ...
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 20000 × g for 10 min at 4°C and added to pre-equilibrated beads (Chromotek, GTA-100) and rotated for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated after centrifugation (13000x g for 10 min at 4°C) and incubated with 25 μl GFP Trap (Chromotek, gtm-20) magnetic beads for 1 hour at 4°C with rotation ...
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bioRxiv - Developmental Biology 2021Quote: ... two different protocols were used: 1) one step of O/N incubation at 4°C with GFP booster (1:250, Alexa Fluor ® 488 Chromotek #gb2AF488), Phalloidin-647 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 50 µL of sepharose beads for 1 h at 4°C with constant rotation and subjected to immunoprecipitation with 25 µL GFP Trap agarose beads (Chromotek, gta-20) during 1 h at 4°C with constant rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 200 µL of sepharose beads for 1 h at 4°C under constant rotation and then subjected to immunoprecipitation with 50 µL of GFP Trap agarose beads (Chromotek, gta-20) for 2 h at 4°C under constant rotation ...
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bioRxiv - Microbiology 2020Quote: ... lysed and immunoprecipitated using a GFP-Trap kit (Chromotek).
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bioRxiv - Biochemistry 2020Quote: ... mCherry-Atg21 and mCherry-WIPI2d bait proteins are incubated at a concentration of 40 μM each with 10 μl RFP-Trap beads (Chromotek) in buffer containing 25 mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was measured using standard Bradford assay and 500 µg of total protein were incubated with GFP-Trap magnetic beads (ChromoTek) for 3h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: Protein extracts containing GFP-tagged EFR or site-directed mutants were incubated with 20 μL of GFP-Trap beads (Chromotek) or GFP-clamp beads (Hansen et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Myc-Trap agarose beads were used to pull down the Myc-TREM2 protein following the manufacturer’s instructions (Chromotek, yta-10). GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414 ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested using the ‘on-bead digest protocol for mass spectrometry following immunoprecipitation with Nano-Traps’ recommended by Chromotek. In both cases mass spectra *.raw files were used to search the A ...
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bioRxiv - Plant Biology 2021Quote: ... 200 mg ground leaf material was subjected to protein extraction and immunoprecipitation using GFP-Trap beads (ChromoTek, IZB Martinsried, Germany) as previously described17.
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bioRxiv - Microbiology 2020Quote: ... equal concentration of total proteins was isolated and incubated with 20~30 μL of GFP-Trap agarose beads (ChromoTek, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining supernatants were used for immunoprecipitation of GFP-fused proteins agarose using GFP-Trap coupled to agarose beads (Chromotek) and following the manufacturer’s instructions ...