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Citations for ChromoTek :
101 - 150 of 435 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... μ 20 mg of total protein was incubated with 15 μl GFP-TrapMA beads (Chromotek) at 4 °C for 2 h ...
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bioRxiv - Microbiology 2021Quote: ... mCherry-fusion proteins were isolated by immunoprecipitation using the RFP-Trap®_MA kit (Chromotek). Lysates from transfected cells were prepared as above and incubated with RFP-trap magnetic agarose beads for 30 min to capture mCherry or mCherry-Lpg2411 ...
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bioRxiv - Cell Biology 2022Quote: eYFP-tagged fusion proteins were immunoprecipitated using anti-GFP magnetic agarose beads (Chromotek, gtma-20). After induction of gene expression of lentiviral transduced KO2 cells with 1 µg/ml doxycycline ...
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bioRxiv - Plant Biology 2022Quote: ... 100 μg SIRK1-GFP protein extract was prebound to GFP-Traps®-MA beads (Chromotek) for two hours under rotation at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... Cleared lysate (700 µL, approximately 0.8 mg total proteins) were incubated with GFP-Trap (Chromotek) for 30 min at room temperature under gentle rotation ...
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bioRxiv - Genetics 2019Quote: ... For IP 25 μL of Red Fluorescent Protein (RFP)-Trap Magnetic Agarose (MA) beads (Chromotek) were added and 25 μL of MA beads (Chromotek ...
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bioRxiv - Cell Biology 2020Quote: ... GFP-tagged proteins were immunoprecipitated using the GFP-Trap Agarose® beads (Chromotek; gta-20) according to the manufacture’s protocol and eluted with 2x Laemmli Sample Buffer (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates from cells expressing GFP tagged proteins were immunoprecipitated using a GFP-Trap Kit (Chromotek) as per manufacturer’s instructions and immunoblotting performed as above ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were immunoprecipitated by the addition of 50 μl pre-equilibrated GFP-Trap resin (Chromotek) and inverted for 2 hours at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: Target proteins were immunoprecipitated either with GFP- or RFP-trap Magnetic Agarose beads (Chromotek, Proteintech) or with Dynabeads Protein G (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... MYC-tagged protein was purified from transduced cells (see above) with Myc-trap agarose (Chromotek), a nanobody based system ...
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bioRxiv - Microbiology 2021Quote: ... Protein extracts were then subjected to immunoprecipitation using agarose beads coupled with anti-RFP nanobodies (Chromotek). Eluates were collected and analysed by western blot ...
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bioRxiv - Plant Biology 2019Quote: ... Total proteins were extracted and immunoprecipitation was performed with GFP-trap beads (Chromotek, Planegg-Martinsried, Germany). Proteins were stripped from the beads by boiling in 50 μl SDS loading buffer for 10 mins ...
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bioRxiv - Cell Biology 2022Quote: ... with equal amounts of total protein extract for each condition incubated with GFP-Trap_A beads (Chromotek) at 4°C for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... to equalise protein concentrations across the samples before immunoprecipitation with GFP-Trap resin (gta-20, ChromoTek) or anti-HA affinity matrix (11815016001 ...
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bioRxiv - Plant Biology 2022Quote: ... leaf material was harvested and protein extracts were subjected to immunoprecipitation using GFP-Trap beads (ChromoTek). 200 mg ground leaf material was subjected to protein extraction and immunoprecipitation using GFP-Trap beads (ChromoTek)19 ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Cell Biology 2024Quote: ... 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek) and incubated for 2 hours at 4°C with gentle agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Nano-secondary AF568 (Chromotek, #srbAF568-1-100, 1:1000) diluted in PBS 10% (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: ... Nano-secondary AF568 (Chromotek, #srbAF568-1-100, 1:1000) prepared in PBS with 10% (wt/vol ...
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bioRxiv - Biophysics 2019Quote: ... Presence of the mNeonGreen protein was probed using a monoclonal mNeonGreen antibody [32F6] (Chromotek; Planegg-Martinsried, Germany) at a 1:1000 dilution and visualized using a DyLight 680 secondary antibody (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... GFP-tagged proteins were immunoprecipitated by incubation with 25 µl of GFP-Trap magnetic agarose beads (Chromotek) overnight at 4 °C on a tube rotator ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extraction and immunoprecipitation were performed as described previously (Kadota et al., 2014) using GFP-trap (Chromotek) or GFP-clamp (Hansen et al. ...
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bioRxiv - Plant Biology 2021Quote: ... The protein extracts were incubated with 20 μL of GFP-Trap magnetic agarose beads (GFP-TrapMA, Chromotek) for 2 h at 4 °C by vertical rotation (10 rpm) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were immunoprecipitated by incubation with anti-HA sepharose beads (Pierce) or anti-GFP-Trap beads (ChromoTek) for 2 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... To each 15 ml of protein extract 40 μl of GFP-TRAP AGAROSE BEADS (50 % slurry, ChromoTek) washed in extraction buffer were added and incubated with gentle agitation for 4 h at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... were proportioned for analysis of protein expression (5%) and immunoprecipitation (95%) using GFP-Trap beads (ChromoTek, Germany). Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Overexpressed protein was immunoprecipitated from the cell lysate using tag specific nano-trap (SPOT-Trap-MA, Chromotek) and analysed by WB.
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bioRxiv - Cell Biology 2021Quote: ... cleared lysates containing GFP-tagged proteins were added to anti-GFP agarose beads (GFP-Trap, ChromoTek, Germany) and incubated while rotating end over end for an hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... an equal amount of total protein extract for each condition was incubated with GFP-Trap_A beads (Chromotek) at 4°C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... Total proteins were inoculated with 30 μL GFP-Trap®_A beads (ChromoTek Inc., Hauppauge, NY, USA) at 4°C for 4h and then the bound proteins eluted by heating at 100°C for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... 200 μg of the extracted proteins were added to 25 μl of equilibrated magnetic agarose beads (Chromotek) and mixed in a rotating shaker for 45 min at 4 degrees ...
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bioRxiv - Plant Biology 2022Quote: ... 200 mg ground leaf material was subjected to protein extraction and immunoprecipitation using GFP-Trap beads (ChromoTek)19 ...
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bioRxiv - Molecular Biology 2023Quote: ... the GFP-fused substrate proteins were immunoprecipitated from the cell extract using GFP-Trap A beads (Chromotek) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... (1:1000, Chromotek) primary antibodies and IRDye® 680RD Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: IPs with GFP-tagged proteins were performed with GFP-binding magnetic agarose beads (GFPtrap MA, Chromotek, #gtma-20). Per IP sample ...
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bioRxiv - Genetics 2020Quote: ... Four milligrams of total protein was mixed with 20 μl of GFP-conjugated agarose beads (#gta-20, Chromotek) equilibrated by washing in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2022Quote: ... Total proteins were then incubated with 30 μL GFP-Trap®_A beads (ChromoTek Inc., Hauppauge, NY, USA) at 4°C for 4h ...
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bioRxiv - Plant Biology 2023Quote: ... Five hundred microliters of the supernatant was incubated with 10µL of green fluorescent protein (GFP)-Trap A (Chromotek) bead slurry for 3 hours at 4°C with constant end-over-end rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Cleared supernatant containing roughly 5.4-6.6 mg of protein was mixed with 50 µl GFP-Trap-A agarose beads (Chromotek), which had been equilibrated in dilution buffer [10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble proteins were separated from the cell debris by centrifugation and incubated with GFP-Trap agarose beads (Chromotek) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 23225) and equal protein from each sample was loaded into pre-conditioned GFP-Trap A beads (gta-20; ChromoTek). Aliquots of the input sample(I ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were cleared by centrifugation (13000 g, 30 min) and the proteins were captured with GFP-trap (Chromotek), Myc-trap (Chromotek ...
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were then incubated for 1.5 h at 4 ⁰C with 25 µL RFP-Trap agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were incubated in primary antibody (mouse monoclonal anti-PDF C7, 1:500-1:2000, DHSB, donated by Justin Blau; rabbit polyclonal anti-GFP 1:1000-2000, ChromoTek) solution in PBT+5% NGS at least overnight at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... rabbit anti-GFP (1:1000) and mouse anti-RFP (1:2000) (ChromoTek) monoclonal primary antibodies were used in combination with anti-rabbit or anti-mouse IgG horseradish peroxidase conjugated secondary antibodies ...