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Citations for ChromoTek :
1 - 50 of 324 citations for Human G Protein Coupled Receptor Family C Group 6 Member A GPRC6A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... GFP tagged proteins was detected with GFP-booster coupled with Atto488 (1:500; Chromotek). DNA was stained by DAPI (0.2 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Protein extracts were then subjected to immunoprecipitation using agarose beads coupled with anti-RFP nanobodies (Chromotek). Eluates were collected and analysed by western blot ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were incubated for 2 hours at 4°C with 25 μL GFP-Trap coupled agarose beads (Chromotek). After incubation ...
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bioRxiv - Plant Biology 2024Quote: Immunoprecipitation was performed by adding 1 g of homogenized leaf material 50 µL of GFP-Trap® coupled to magnetic beads (ChromoTek). Samples were processed as described (Raffeiner et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 15 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 150 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were cleared by centrifugation (6,000 g, 30 min, 4°C) and incubated with GFP-Trap (Chromotek) for 2h ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining supernatants were used for immunoprecipitation of GFP-fused proteins agarose using GFP-Trap coupled to agarose beads (Chromotek) and following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins (GFP-Trap; ChromoTek GmbH, Germany; gta-20) was used for immunoprecipitation of mCerN1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Neuroscience 2020Quote: ... GFP fusion proteins in fixed cells were labelled with Alexa Fluor 647-coupled GFP nanobodies (GFP-booster gb2AF647-50, Chromotek, Germany) at 1:100 dilution ...
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bioRxiv - Plant Biology 2021Quote: One milligram of total protein extract was mixed with 25 μl of GFP Nanobody/VHH coupled to agarose beads (GFP-Trap® Agarose, Chromotek) and incubated for 3 hours and 30 minutes at 4°C with end to end rocking ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on 20 μl GFP-Trap beads (ChromoTek) for 30 min at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by filtering through miracloth followed by centrifugation (6,000 g, 30 min, 4°C) and then incubated with GFP-Trap (Chromotek) for 4h ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were collected (centrifugation for 10 min at 21,100 g and 4 °C) and the supernatant was added to 25 µl of blocked GFP-Trap Agarose (gta, ChromoTek).
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bioRxiv - Cell Biology 2023Quote: ... Cell lysate was centrifuged at 17,000 g for 10 min at 4 °C and supernatant was applied to 20 μl of GFP-Trap beads (Chromotek) and shaken for 2 hr at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... α-GFP antibody-coupled magnetic beads (Chromotek) were equilibrated with IP buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were cleared by centrifugation (13000 g, 30 min) and the proteins were captured with GFP-trap (Chromotek), Myc-trap (Chromotek ...
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bioRxiv - Cell Biology 2020Quote: ... To enhance signal from fluorescent reporters we used GFP-booster coupled to Atto488 (gba488) and RFP-booster coupled to Atto594 (rba594) from Chromotek. Secondary antibodies used were from Thermo Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 20000 × g for 10 min at 4°C and added to pre-equilibrated beads (Chromotek, GTA-100) and rotated for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated after centrifugation (13000x g for 10 min at 4°C) and incubated with 25 μl GFP Trap (Chromotek, gtm-20) magnetic beads for 1 hour at 4°C with rotation ...
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bioRxiv - Plant Biology 2023Quote: ... or Myc-antibody coupled beads (Myc-trap, ChromoTek) were used ...
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bioRxiv - Immunology 2023Quote: The coding sequence of the Dnmt1-chromobody targeting the human Dnmt1 protein [37] (Uniprot : P26358 · DNMT1_HUMAN) and lamin-chromobody (ChromoTek) targeting the lamin D0 from Drosophila monogaster (Uniprot ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Neuroscience 2020Quote: ... with rotation with anti-GFP-coupled magnetic beads (Chromotek). The beads were washed with lysis buffer and 1 M urea before Western blot analysis ...
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bioRxiv - Cell Biology 2023Quote: ... One third of the whole cell lysate was pre-cleared with a 50:50 mixture of protein A/G magnetic beads and GFP-Trap (Chromotek, gtd-20) was pre-blocked with 1mg/mL ultra-pure BSA (AM2616 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were then incubated for 1.5 h at 4 ⁰C with 25 µL RFP-Trap agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Plant Biology 2020Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... 50μg of total proteins were incubated 3H at 4°C with 30μl of GFP-Trap Agarose beads (Chromotek; gta-20) under gentle agitation for GFP-tagged protein purification ...
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bioRxiv - Plant Biology 2022Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation was performed using the protein lysates and a GFP-Trap_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed using the protein lysates and a GFP-Trap_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... nanobody boosters towards GFP (anti-GFP, Atto-488 coupled, Chromotek; gba488-100) were used to enhance the signal ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with RFP-Booster nanobody coupled to Atto 594 (Chromotek) at a dilution of 1:200.
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation was performed using crosslinked protein lysate and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... mCherry-fusion proteins were isolated by immunoprecipitation using the RFP-Trap®_MA kit (Chromotek). Lysates from transfected cells were prepared as above and incubated with RFP-trap magnetic agarose beads for 30 min to capture mCherry or mCherry-Lpg2411 ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates from cells expressing GFP tagged proteins were immunoprecipitated using a GFP-Trap Kit (Chromotek) as per manufacturer’s instructions and immunoblotting performed as above ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatants were incubated 1h with GFP-Trap coupled to magnetic agarose beads (Chromotek). Then ...