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Citations for ChromoTek :
1 - 50 of 88 citations for HDM Fluorescent Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
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Cellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona larvabioRxiv - Neuroscience 2020Quote: ... rat anti-red fluorescent protein (RFP) monoclonal (5F8; ChromoTek GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescent lamin nanobody (lamin chromobody) plasmid was purchased from ChromoTek and transferred into the NcoI-XbaI site of pGEMHE ...
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bioRxiv - Neuroscience 2022Quote: ... rat monoclonal anti-red fluorescent protein (RFP) (Chromotek, 1:1000) and goat anti-mCherry (1:1000) ...
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bioRxiv - Neuroscience 2022Quote: ... red fluorescent protein (RFP; rat, Chromotek: 5F8, RRID: AB_2336064; 1:2000) FoxP2 (mouse ...
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bioRxiv - Plant Biology 2022Quote: ... rat anti-red fluorescent protein (RFP) (5F8, Chromotek, Planegg-Martinsried, Germany) (1:10,000) ...
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bioRxiv - Biophysics 2023Quote: ... Purified recombinant Enhanced Green Fluorescent Protein (EGFP) was purchased from Chromotek. Bafilomycin A1 was purchased from InvivoGen ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescent nuclear actin nanobody (nuclear actin chromobody) plasmid was purchased from ChromoTek and transferred into the HindIII-EcoRI site of pGEMHE ...
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bioRxiv - Cell Biology 2019Quote: ... The mCherry fluorescent signal was enhanced with rat mAb anti-RFP (Chromotek). As secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... and a red fluorescent protein tag (tdTomato; ChromoTek, 5f8-100, 1:500) expressed by the rabies virus ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was incubated with 10µL of green fluorescent protein (GFP)- Trap A (Chromotek) bead slurry for 4 hours at 4°C with constant slow rotation followed by washing the bead slurry 5 times with 500µL IP wash buffer at 1,000 x g for 1 minute at 4°C ...
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bioRxiv - Genetics 2019Quote: ... For IP 25 μL of Red Fluorescent Protein (RFP)-Trap Magnetic Agarose (MA) beads (Chromotek) were added and 25 μL of MA beads (Chromotek ...
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bioRxiv - Plant Biology 2023Quote: ... Five hundred microliters of the supernatant was incubated with 10µL of green fluorescent protein (GFP)-Trap A (Chromotek) bead slurry for 3 hours at 4°C with constant end-over-end rotation ...
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Cell Biology 2020Quote: ... To enhance signal from fluorescent reporters we used GFP-booster coupled to Atto488 (gba488) and RFP-booster coupled to Atto594 (rba594) from Chromotek. Secondary antibodies used were from Thermo Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... and mass spectrometry compatibility (plates are now commercially available as: GFP-Trap® Multiwell Plate, cat no. gtp-96, Chromotek GmbH, Martinsried, Germany). Plates were prepared with 200 μL wash buffer 1 (50 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... The nanoboosters - single-domain alpaca antibody fragments covalently coupled to fluorescent dyes — GFP-Booster Atto488 and RFP-Booster Atto594 (Chromotek, Martinsried, Germany) — were used to boost eYFP and mCherry fluorescence ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Plant Biology 2021Quote: ... and serine 2 (Chromotek, diluted 1:200). After washing with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... GFP-Trap magnetic agarose beads (20 μL in first plate only; Cat.#GTMA-10, Chromotek) and/or up to 1.0 mL buffer were pre-aliquoted into 96 Deep-well plates ...
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bioRxiv - Cell Biology 2021Quote: ... Myc-Trap agarose beads A (Chromotek, yta-2 Myc Trap_A) were prepared by 3 rounds of washing in extraction buffer and centrifugation at 1000 x g for 5min at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: Cells from an 80% confluent 10-cm plate were harvested and the immunoprecipitation protocol from Chromotek was followed ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then transferred in 900 μl aliquots to fresh Eppendorf tubes and incubated with gentle head-over-tail agitation for 2 h at 4°C with 30 μl of anti-RFP or control beads (Chromotek rtma-2 and bmab-20, respectively) that had been washed in 1x lysis buffer.
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bioRxiv - Biochemistry 2019Quote: ... were immunoprecipitated from 2 mg of lysates using GFP-Trap beads (Chromotek) at 4°C for 4h under rotation ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... GFP-Trap®_MA kit (Chromotek) or Pierce™ Anti-HA Magnetic Beads (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A GFP-TRAP agarose kit (Chromotek) was used to purify GFP-tagged protein from infected HaCat cells according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The GFP-Trap agarose kit (Chromotek) was used for immunoprecipitation according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... cleared lysate was incubated with 2 μl washed GFP-Trap magnetic agarose beads (Chromotek) per well for >4 h at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... S2Lb-GFP proteins were immunoprecipitated for 2 hours using a GFP-trap system (Chromotek) coupled to magnetic beads ...
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bioRxiv - Neuroscience 2024Quote: ... Immunocytochemistry was performed with the following primary antibodies: rat anti-GFP (Chromotek, 50430-2-AP) 1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... and Myc-Trap®_A Kit (Chromotek) respectively ...
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bioRxiv - Plant Biology 2024Quote: ... GFP-trap beads (GFPTrap-A kit, Chromotek) were equilibrated with dilution buffer (same as the extraction buffer except for the omission of Triton X-100) ...
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bioRxiv - Microbiology 2023Quote: ... One plate (∼5x107 cells) per condition was harvested using RIPA lysis and immunoperciptated with GFP-trap agarose beads (ChromoTek ref# gta- 10) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... rat antibodies for the detection of phosphorylated RNAPII (serine 5 and serine 2) (Chromotek, 1: 100) and secondary goat anti-rat Alexa Fluor 488 antibodies (A-11006 ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were incubated (2 h, at 4°C) with 20 µl GFP-Trap®_MA (ChromoTek). Beads were then washed 3 times for 10 min with 1 ml of IP buffer and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2022Quote: ... immunoprecipitation was performed using GFP-Trap Kit (Chromotek) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... an mNeonGreen-Trap Agarose Kit (ntak-20; Chromotek) to pull down Tld1-mNeonGreen (mNG ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 2 in CTD domain rat 3E10 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Immunology 2023Quote: ... 2 mg of cell lysates were incubated with 25 ml of GFP-TRAP beads (Chromotek, GTA-20) for 1 hr at 4°C on rotation ...
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bioRxiv - Microbiology 2020Quote: ... lysed and immunoprecipitated using a GFP-Trap kit (Chromotek).
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bioRxiv - Cell Biology 2019Quote: ... lysates were incubated for 2 hours at 4°C with 25 μL GFP-Trap coupled agarose beads (Chromotek). After incubation ...
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bioRxiv - Genomics 2020Quote: ... at 4°C or for 2 hours with 20 uL of GFP-Trap magnetic beads (Chromotek, gtma-20) at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a 1:1,000 dilution of mouse anti-GFP [B-2] (SantaCruz Biotechnology) or a rat anti-RFP [5F8] (Chromotek) followed by a 1:5,000 dilution of the horseradish peroxidase-conjugated secondary antibody against mouse IgG (7076 ...
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bioRxiv - Cell Biology 2020Quote: ... For IP 2 mg of cytoplasmic and nuclear extracts were incubated with either 15 µl GFP Trap beads (Chromotek) for 1 h at 4°C or 1-1.5 μg IgG overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Cell Biology 2023Quote: ... Insoluble material was removed by centrifugation at 14,000 x g for 2 minutes and the supernatant was incubated with 50 µl HNG-washed agarose beads (Chromotek) with end-over-end rotation for 15 minutes to capture non-specific binding proteins ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 mM dithiobis [succinimidyl propionate] containing 1x Complete Protease Inhibitor Cocktail) and then incubated with GFP-TRAP agarose beads (ChromoTek) for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...