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Citations for ChromoTek :
201 - 250 of 258 citations for Dengue Virus Serotype 4 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Immunology 2023Quote: The coding sequence of the Dnmt1-chromobody targeting the human Dnmt1 protein [37] (Uniprot : P26358 · DNMT1_HUMAN) and lamin-chromobody (ChromoTek) targeting the lamin D0 from Drosophila monogaster (Uniprot ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 20000 × g for 10 min at 4°C and added to pre-equilibrated beads (Chromotek, GTA-100) and rotated for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated after centrifugation (13000x g for 10 min at 4°C) and incubated with 25 μl GFP Trap (Chromotek, gtm-20) magnetic beads for 1 hour at 4°C with rotation ...
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bioRxiv - Developmental Biology 2021Quote: ... two different protocols were used: 1) one step of O/N incubation at 4°C with GFP booster (1:250, Alexa Fluor ® 488 Chromotek #gb2AF488), Phalloidin-647 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 50 µL of sepharose beads for 1 h at 4°C with constant rotation and subjected to immunoprecipitation with 25 µL GFP Trap agarose beads (Chromotek, gta-20) during 1 h at 4°C with constant rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 200 µL of sepharose beads for 1 h at 4°C under constant rotation and then subjected to immunoprecipitation with 50 µL of GFP Trap agarose beads (Chromotek, gta-20) for 2 h at 4°C under constant rotation ...
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bioRxiv - Biochemistry 2020Quote: ... mCherry-Atg21 and mCherry-WIPI2d bait proteins are incubated at a concentration of 40 μM each with 10 μl RFP-Trap beads (Chromotek) in buffer containing 25 mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was measured using standard Bradford assay and 500 µg of total protein were incubated with GFP-Trap magnetic beads (ChromoTek) for 3h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: Protein extracts containing GFP-tagged EFR or site-directed mutants were incubated with 20 μL of GFP-Trap beads (Chromotek) or GFP-clamp beads (Hansen et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Myc-Trap agarose beads were used to pull down the Myc-TREM2 protein following the manufacturer’s instructions (Chromotek, yta-10). GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414 ...
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bioRxiv - Plant Biology 2021Quote: ... thaliana plants were incubated with 50-100 μl previously washed 50% GFP-binding protein beads slurry (Chromotek, GFP-Trap®) at 4 °C for 3 hours on a roller mixer ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested using the ‘on-bead digest protocol for mass spectrometry following immunoprecipitation with Nano-Traps’ recommended by Chromotek. In both cases mass spectra *.raw files were used to search the A ...
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bioRxiv - Plant Biology 2021Quote: ... 200 mg ground leaf material was subjected to protein extraction and immunoprecipitation using GFP-Trap beads (ChromoTek, IZB Martinsried, Germany) as previously described17.
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bioRxiv - Microbiology 2020Quote: ... equal concentration of total proteins was isolated and incubated with 20~30 μL of GFP-Trap agarose beads (ChromoTek, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining supernatants were used for immunoprecipitation of GFP-fused proteins agarose using GFP-Trap coupled to agarose beads (Chromotek) and following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted as indicated above and immunoprecipitation was performed with 15 μL of GFP-trap beads (ChromoTek, Germany) or ANTI-FLAG M2 Affinity Agarose Gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... Clover- and Cherry-tagged proteins were immunoprecipitated by incubation with either GFP-(gta) or RFP-Trap(rta) magnetic beads/agarose (Chromotek) for 1 h at 4°C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... the extract prepared from OSC lines expressing endogenously tagged proteins of interest was incubated with magnetic GFP-Trap agarose (Chromotek) for 3h at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and subjected to immunoprecipitation of RFP/mCherry/dsRed-tagged or GFP-tagged fusion proteins using RFP-trap or GFP-trap matrix (Chromotek), respectively ...
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bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mg of the total proteins were incubated with 20 μL of GFP-Trap magnetic agarose beads (GFP-TrapMA; Chromotek) for 2 hours at 4°C by vertical rotation ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins (GFP-Trap; ChromoTek GmbH, Germany; gta-20) was used for immunoprecipitation of mCerN1 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mL of the protein supernatant was incubated with 10 μL of magnetic GFP-Trap beads (Chromotek, Cat. No. gtma) or magnetic Myc-Trap beads (Chromotek ...
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bioRxiv - Developmental Biology 2023Quote: ... A maximum of 500 ug total protein in a volume of 950 ul was loaded per 25 ul GFP-Trap agarose beads (gta, Chromotek), prior washed with ice-cold PBS (2 min at 2’500 x g ...
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bioRxiv - Plant Biology 2024Quote: ... A volume of 2 mL of total protein was mixed with 25 μL of GFP-TRAP agarose beads (50% slurry, ChromoTek) and incubated shaking overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... To avoid unspecific protein binding the worm lysate was pre-cleared by incubation to uncoupled agarose beads (Chromotek, bab-20) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2024Quote: Protein extracts containing GFP-tagged MIK2 or site-directed mutants were incubated with 20 µL of GFP-Trap beads (Chromotek) for 2 h with gentle mixing at 4 °C to immuno-precipitate receptor complexes ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were measured and 200 µg of lysates were subjected to immunoprecipitation with GFP-Trap®_MA magnetic beads (Chromotek) at 4° C for two hours on a rotating wheel ...
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bioRxiv - Microbiology 2019Quote: ... The Protein A agarose beads were pelleted by centrifugation and the supernatant was incubated with GFP-Trap® agarose beads (Chromotek) for 4 h at 4 °C on a mixing wheel ...
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bioRxiv - Neuroscience 2020Quote: ... GFP fusion proteins in fixed cells were labelled with Alexa Fluor 647-coupled GFP nanobodies (GFP-booster gb2AF647-50, Chromotek, Germany) at 1:100 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... and 500 µg of protein from each sample was incubated with 35 µL pre-washed GFP-Trap magnetic beads (Chromotek, Inc.) for 1 h at 4 °C with rotation (1 mg/mL final lysate concentration) ...
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bioRxiv - Plant Biology 2021Quote: One milligram of total protein extract was mixed with 25 μl of GFP Nanobody/VHH coupled to agarose beads (GFP-Trap® Agarose, Chromotek) and incubated for 3 hours and 30 minutes at 4°C with end to end rocking ...
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bioRxiv - Cell Biology 2022Quote: ... Then 800-1000 µg of total protein lysates were added to 25 μl of anti-GFP magnetic beads (ChromoTek GFP-Trap) and tumbled end-over-end for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: For CoIP experiments 500 μl protein extract was mixed with 25 μl calibrated anti-GFP magnetic beads (ChromoTek GFP-Trap®) and incubated on a rotator for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Plant Biology 2020Quote: Blotted proteins were detected using anti-GFP (LifeTechnologies, Darmstadt, Germany) and anti-red FP (RFP) antibody (ChromoTek GmbH, Planegg-Martinsried, Germany) (both ...
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bioRxiv - Cell Biology 2023Quote: ... and approximately 250 µg of protein was incubated with anti-GFP-conjugated magnetic agarose beads (ChromoTek GFP-Trap® Magnetic Agarose) for 1-1.5 hours ...
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bioRxiv - Plant Biology 2024Quote: ... between EIN2-C and HAF2 following study previous briefly: Total protein extracts from plants mentioned in the paper were immunoprecipitated by DYKDDDDK Fab-Trap™ agarose beads (ChromoTek) for two hours at 4 °C with gentle rotating and then beads were washed five times with ice-cold 1XPBS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then probed overnight at 4°C with TBST buffer containing 1% non-fat dry milk and primary antibodies: mouse monoclonal anti-RFP (Chromotek, clone 6G6, 1:3,000), rabbit polyclonal anti-GFP (SYSY ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Twenty milligram protein extracts were taken as input and the rest of the extracts were incubated with the GFP-Trap beads (ChromoTek, gta-20) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates (200 μg protein) from HEK-293T cells expressing WT-TRPC6-YFP was incubated with GFP M-trap beads (Chromotek GTD-20) to immunoprecipitate TRPC6 ...
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bioRxiv - Plant Biology 2019Quote: ... The protoplasts were collected and the subsequent work was operated following the protocol for immunoprecipitation of Myc-fusion proteins using Myc-Trap®_MA (Chromotek, Germany). Both the input and the IP samples were separated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of the protein lysates was incubated for 2 h with 10 µl of either Myc-Trap® or GFP-Trap® beads (Chromotek). To determine the dependence of interactions on RNA ...
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bioRxiv - Microbiology 2019Quote: ... washed twice with ice-cold PBS and immunoprecipitation of GFP-tagged proteins was performed using GFP-Trap®_A kit (Chromotek, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mL of protein extract was incubated with 60 μL (50% slurry) of GFP-trap magnetic agarose beads (ChromoTek, Planegg-Martinsried, Germany) for 3 h on a rotary shaker at 4°C ...