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Citations for ChromoTek :
1 - 50 of 261 citations for Chikungunya Virus VLP E1 E2 C protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were then incubated for 1.5 h at 4 ⁰C with 25 µL RFP-Trap agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
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bioRxiv - Plant Biology 2020Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... 50μg of total proteins were incubated 3H at 4°C with 30μl of GFP-Trap Agarose beads (Chromotek; gta-20) under gentle agitation for GFP-tagged protein purification ...
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bioRxiv - Plant Biology 2022Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... anti-GFP conjugated to Atto488 (1:100; ChromoTek gba-488; Fig. 3B-C, S2A,C), anti-mouse secondary antibodies (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Microbiology 2022Quote: GFP-tagged target proteins and interacting proteins were affinity purified using GFP-trap agarose beads (Chromotek). Proteins were processed ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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bioRxiv - Plant Biology 2020Quote: ... or c-Myc rat monoclonal antibody (Chromotek, 9E1-100) at a dilution of 1:1000 followed by goat anti-rat IgG horseradish peroxidase (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined and an equal amount of protein was mixed with TurboGFP-Trap Magnetic Agarose (Chromotek) for 1 hour at RT with agitation ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were detected using α-HA (Chromotek) and α-GFP antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... GFP fusion proteins were purified as follows: 200mg of protein extract was incubated with GFP-Trap Magnetic Agarose (ChromoTek) at 4°C for 20 minutes with gentle agitation ...
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bioRxiv - Plant Biology 2023Quote: GFP-tagged or RFP-tagged proteins were immunoprecipitated from total leaf protein extract by incubation with GFP-Trap or RFP-Trap magnetic agarose beads (ChromoTek). The Co-immunoprecipitation assay was carried out according to ChromoTek’s instructions whereby 300 μl of protein extract was diluted with 500 μl of ice-cold dilution buffer [10 mM Tris-HCl (pH 7.5),150 mM NaCl ...
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bioRxiv - Neuroscience 2019Quote: ... and 100 ng/ml GFP-Trap Protein (ChromoTek). Tissue samples were homogenized three times at 250 rpm and ten times at 750 rpm on a variable-speed homogenizer (Glas-Col ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were purified by GFP-trap (ChromoTek) immunoprecipitation for 1 hour at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Protein complexes were immunoprecipitated using GFP-Trap (Chromotek) and washed 3 times with 50mM ammonium bicarbonate after the precipitation to remove detergent ...
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bioRxiv - Systems Biology 2023Quote: ... The proteins were immunoprecipitated using GFP-Trap (Chromotek) according to manufacturer’s protocol (with slight modifications) ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were incubated with GFP-Trap (ChromoTek) beads for 1h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... C GFP-Trap (Figure S3B) or RFP-Trap (Figure S3C) beads (Chromotek) were first coated with the ATG12–ATG5-ATG16L1-GFP protein (Figure S3B ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200 ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-c-myc beads (VF299569, Thermo) or RFP-Trap Magnetic Agarose (Chromotek) were added to the protein extracts and incubated at 4°C for 3 h ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Developmental Biology 2019Quote: ... Tagged proteins were immunoprecipitated with Myc-Trap_MA beads (ChromoTek) or anti-FLAG M2 Affinity gel (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... they were incubated with unlabelled (GFP-binding protein, Chromotek) and Abberior 635P conjugated Nanobody (GFP-booster ...
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bioRxiv - Cell Biology 2021Quote: ... protein lysates were added to RFP-Trap beads (Chromotek) and incubated for one hour at 4°C on a rotating shaker ...
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Cellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona larvabioRxiv - Neuroscience 2020Quote: ... rat anti-red fluorescent protein (RFP) monoclonal (5F8; ChromoTek GmbH ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using anti-GFP antibody (3H9, Chromotek) or anti-alpha tubulin (T5168 ...
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bioRxiv - Plant Biology 2023Quote: ... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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bioRxiv - Plant Biology 2024Quote: ... Coimmunoprecipitation of GFP- or RFP-tagged proteins from total leaf protein extract by incubation with GFP- or RFP-Trap magnetic agarose beads (ChromoTek, Planegg-Martinsried Germany) was performed as previously reported (Azevedo et al. ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated at 4°C overnight with GFP-Trap agarose (Chromotek) or Dynabeads (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were immunoprecipitated at 4°C in lysis buffer with GFP-Trap (ChromoTek) beads as described by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Complexes were immunoprecipitated at 4°C in lysis buffer with GFP-Trap (ChromoTek) and washed in lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were immunoprecipitated with GFP-Trap beads (Chromotek, Germany), and analyzed by western blot with anti-GFP (Abiocode ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged proteins were affinity purified by GFP-trap (ChromoTek) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The protein complex pulled down by GFP-Trap beads (Chromotek) was washed three times with RIPA buffer and eluted in 30 μl of 1x Laemmli sample buffers by heating at 75°C for 10 min.
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bioRxiv - Cell Biology 2022Quote: ... GFP-GABARAP proteins were immunoprecipitated using GFP-TRAP beads (ChromoTek), incubating for 2hrs at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... rat monoclonal anti-red fluorescent protein (RFP) (Chromotek, 1:1000) and goat anti-mCherry (1:1000) ...
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bioRxiv - Microbiology 2023Quote: GFP-tagged proteins were immunoprecipitated using GFP-TRAP beads (Chromotek) using TNTE buffer (20mM Tris-HCL pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... or the 32F6 IgG2c monoclonal antibody against mNeonGreen protein (Chromotek). After several 5 min washes in PBS ...
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bioRxiv - Genetics 2023Quote: Protein complex was purified using GFP-Trap agarose beads (Chromotek) and separated by western blot ...
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bioRxiv - Plant Biology 2023Quote: ... protein extracts were incubated with GFP-Trap Agarose (Chromotek, Germany) at 4°C for 1 h with gentle rotation ...
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by GFP-Trap Agarose (Chromotek, gta).
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by RFP-Trap Agarose (Chromotek, rta). pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S: ...
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bioRxiv - Plant Biology 2024Quote: ... GFP-tagged proteins were detected by α-GFP antibodies (ChromoTek).