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Citations for ChromoTek :
151 - 188 of 188 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The blot was incubated overnight on a shaker (4°C) with the primary antibody (anti-GFP 3H9, anti-RFP 6G6 (Chromotek) or anti-tRFP (Evrogen)) ...
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bioRxiv - Genetics 2021Quote: ... Pulldowns of the GFP-fusion proteins were performed by incubating tissue homogenate (7-day-old seedlings) or cell lysate (CHO cell lysate) with agarose beads that conjugated with GFP antibodies (GFP Nano-Trap, Chromotek). After incubations of 4-24 hours with gentle inverting ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with primary antibody solution containing rabbit a-HA (1:500) (Cell Signalling) and rat a-RFP (1:500) (Chromotek) diluted in 3% BSA in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... portions of the blot were reacted against anti- mNeonGreen (mouse monoclonal antibody 32F6 from Chromotek, Inc., at 1:200 dilution), anti- HA (rabbit monoclonal antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... The CUT and TAG experiment was done for two biological replicates using antibodies anti-GFP (Chromotek, PABG1-100, 1:50), anti-YFP (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on 8% SDS-PAGE gels and detected by western blot using monoclonal mouse anti-HA (MMS-101R; Covance) or monoclonal mouse anti-RFP antibodies (6G6; ChromoTek) prior to secondary antibody treatment with polyclonal goat anti-mouse conjugated to horseradish peroxidase (115–035-146 ...
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bioRxiv - Cell Biology 2024Quote: ... The insoluble material was removed by centrifugation and the lysates were subjected to immunoprecipitation either by subsequent incubations with anti-Ecad antibody SHE78-7 (4 μg/ml) and protein A-beads or by incubation with GFP-trap beads (Chromotek). After incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation was performed using crosslinked protein lysate and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Co-Immunoprecipitation (Co-IP) experiments were performed using the GFP-Trap®_M kit (ChromoTek). Proteins were extracted by grinding roots of 10-day-old Arabidopsis seedlings expressing pCAMV35S::GFP or pRXR1::RXR1-GFP in liquid N2 ...
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bioRxiv - Microbiology 2021Quote: ... mCherry-fusion proteins were isolated by immunoprecipitation using the RFP-Trap®_MA kit (Chromotek). Lysates from transfected cells were prepared as above and incubated with RFP-trap magnetic agarose beads for 30 min to capture mCherry or mCherry-Lpg2411 ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates from cells expressing GFP tagged proteins were immunoprecipitated using a GFP-Trap Kit (Chromotek) as per manufacturer’s instructions and immunoblotting performed as above ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Slides were subsequently blocked overnight in PBS containing 3% BSA before labelling with anti-mNeonGreen mouse monoclonal antibody (32F6; 1:300; Chromotek, Germany) followed by Alexa Fluor 488 goat anti-mouse antibody (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: Blotted proteins were detected using anti-GFP (LifeTechnologies, Darmstadt, Germany) and anti-red FP (RFP) antibody (ChromoTek GmbH, Planegg-Martinsried, Germany) (both ...
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bioRxiv - Developmental Biology 2023Quote: ... The following antibodies were used: rabbit or rat anti-dsRed (1:200, Clontech 632496 or 5F8 1:400, Chromotek 5f8-100); chicken anti-GFP (1:800 ...
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bioRxiv - Neuroscience 2024Quote: ... incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rat anti-RFP, 1:1000, 5f8, Chromotek; mouse anti-5mC ...
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bioRxiv - Cell Biology 2022Quote: ... Co-immunoprecipitation was performed using a GFP- or RFP-Trap Kit (Chromotek, #gtma-20, #rtma-20) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... GFP-Trap Magnetic Agarose beads or control beads without antibodies were used according to the manufactured protocol (both Chromotek, Planegg-Martinsried, Germany). The loaded magnetic beads were heated for 10 min at 95 °C in SDS loading buffer for the dissociation of purified proteins ...
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bioRxiv - Plant Biology 2021Quote: ... For immunoprecipitations, α-rabbit Trueblot agarose beads (eBioscience) coupled with α-FLS2 antibodies (Chinchilla et al., 2007) or GFP-Trap agarose beads (ChromoTek) were used and incubated with the crude extract for 3-4 h at 4 °C ...
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Mutual repression between JNK/AP-1 and JAK/STAT stratifies cell behaviors during tissue regenerationbioRxiv - Developmental Biology 2022Quote: ... Membrane was incubated with primary antibodies in PBS-T (1% Triton-X in 1xPBS) - rat anti-GFP (Chromotek, 3H9-100, 1:1000) and mouse anti-α-tubulin (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Developmental Biology 2021Quote: ... Explant immunoprecipitation (IP) was then performed using a GFP-Trap Agarose Kit (ChromoTek, catalog number gtak-20) and proteins were eluted in 2X sample buffer ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed and treated for immunofluorescence with α-SINV C antibody at 1:200 dilution and GFP-Booster_Atto488 (ChromoTek GmbH, #gba488-100) at 1:500 dilution following the procedure described in (Garcia-Moreno et al. ...
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bioRxiv - Cell Biology 2024Quote: ... thawed 90-nm-thick cryosections were incubated for 30 min at RT with a rabbit anti-GFP antibody (Chromotek, PABG1, 1:200 dilution) followed by protein A conjugated to 10 nm gold particles for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibody incubation was carried out for 1 hour at room temperature with GFP-Booster Alexa Fluor 488 (Chromotek, gb2AF488, 1:500 dilution) and Alexa Fluor 647 donkey anti-rabbit IgG (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then probed overnight at 4°C with TBST buffer containing 1% non-fat dry milk and primary antibodies: mouse monoclonal anti-RFP (Chromotek, clone 6G6, 1:3,000), rabbit polyclonal anti-GFP (SYSY ...
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bioRxiv - Plant Biology 2020Quote: ... and the protein extract was separated on reducing SDS-PAGE and probed with commercial anti-RFP or anti-GFP antibodies (6G6, Chromotek or ab290, Abcam, respectively) to detect mCherry tagged MSBP1 or GFP-tagged ATI1 ...
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bioRxiv - Cell Biology 2020Quote: ... The cultured explants were collected and immunoprecipitation (IP) was performed using GFP-Trap Agarose Kit (ChromoTek, cat# gtak-20). Immunoprecipitated proteins were eluted in 2X sample buffer ...
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bioRxiv - Molecular Biology 2021Quote: The SLO-treated trophozoite stage lysate of PfHDP-GFP transgenic parasites was immunoprecipitated using GFP-Trap®_A Kit (Chromotek)/ anti-GFP antibody following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Assay Kit (MilliporeSigma, Cat# 17-295) with adaptations based on the application notes on GFP- and RFP-Trap by ChromoTek. 100 Eye-antennal discs for each replicate were dissected in ice-cold 1xPBS ...
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bioRxiv - Plant Biology 2022Quote: Meiotic buds from ∼150 inflorescences from CDKD;3:YFP and wild type control were grinded in liquid nitrogen and processed using the ChromoTek GFP-Trap® Magnetic Agarose kit (Chromotek). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... washed twice with ice-cold PBS and immunoprecipitation of GFP-tagged proteins was performed using GFP-Trap®_A kit (Chromotek, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Cell lysates were cleared and then incubated with GFP-Trap MA beads as instructed in ChromoTek GFP-Trap® MA immunoprecipitation kit (ChromoTek Inc., Hauppauge, NY). Cell lysates were subsequently washed twice in dilution buffer (10 mM Tris-HCl pH 7.5 ...