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Citations for ChromoTek :
101 - 150 of 194 citations for 8 4 dimethylaminophenyl diazenyl N N dimethyl 10 phenylphenazin 10 ium 2 amine chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Both these cell pellet-derived samples and the culture supernatant samples were incubated with 10 μl RFP-trap MA (Chromotek) for one hour at 24 °C with constant mixing ...
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bioRxiv - Cell Biology 2022Quote: ... and the lysate supernatant was added to 25µL of mNeonGreen-Trap Magnetic Agarose suspension (cat. no. ntma-10, Chromotek, Inc.) in which nanobodies to mNeonGreen had been coupled to magnetic agarose beads ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then incubated with 10 μl of a GFP-TRAP-MA beads that were previously washed with lysis buffer (Chromotek). Immunoprecipitation was performed for 1 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cleared lysates were diluted with 5 mL dilution buffer (lysis buffer without NP-40) and incubated with 10 μL bead slurry (GFP-Trap Magnetic Particles M-270, Chromotek) for 2 h ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mg of the total proteins were incubated with 20 μL of GFP-Trap magnetic agarose beads (GFP-TrapMA; Chromotek) for 2 hours at 4°C by vertical rotation ...
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bioRxiv - Biochemistry 2023Quote: ... were purified from three confluent 10-cm dishes of transfected U2OS cells following the above protocol for denaturing immunoprecipitation using Myc-trap beads (ChromoTek). A control purification from cells transfected with empty vector alone was performed in parallel ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mL of the protein supernatant was incubated with 10 μL of magnetic GFP-Trap beads (Chromotek, Cat. No. gtma) or magnetic Myc-Trap beads (Chromotek ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was loaded onto an equilibrated DIY mini-column with DYKDDDDK Fab-Trap™ Agarose resin (250 uL, ffa-10, Chromotek). The column was washed with 6 × 1mL of wash buffer (20 mM TrisCl ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: Co-immunoprecipitation assays and western blots were performed as described previously (Kumar et al., 2021) using GFP trap magnetic agarose beads (Chromotek, gtma-10).
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation of tagged PC1 was done using anti-GFP nanobodies coupled to magnetic agarose beads (GFP-trap, Chromotek cat. no. gtma-10) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Plant Biology 2022Quote: ... and the remaining was supplied with 740 µL extraction buffer and then incubated with GFP-Trap (Chromotek, 10 µL for each sample) for 2 hours with gentle shaking ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mL of protein extract was incubated with 60 μL (50% slurry) of GFP-trap magnetic agarose beads (ChromoTek, Planegg-Martinsried, Germany) for 3 h on a rotary shaker at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... One plate (∼5x107 cells) per condition was harvested using RIPA lysis and immunoperciptated with GFP-trap agarose beads (ChromoTek ref# gta- 10) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 8 µL (35 μL for ChIP-seq) of GFP-Trap magnetic agarose beads (Chromotek) were equilibrated in PBB buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 4 hours at 4°C with agarose-conjugated GFP-Trap™ beads (Chromotek). Beads were washed once with lysis buffer ...
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bioRxiv - Pathology 2021Quote: ... 1-4 mL of supernatant was incubated at 4°C with GFP-Trap_A beads (Chromotek, Planegg-Martinsried, Germany) overnight ...
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bioRxiv - Plant Biology 2019Quote: ... or pS2Lb::S2Lb-GFP 10-day-old seedlings as described above using either GFP-or MYC-TRAP slurries (Chromotek # gtma-20 and #yta-20, respectively) in modified RIPA buffer to allow for SDG2 affinity purification ...
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bioRxiv - Biochemistry 2023Quote: ... The rest of the supernatant was mixed with 8 µL anti-GFP beads (Chromotek, gta-20). The mixture was incubated for 2 h at 4 °C while rotating ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed in the same buffer with 0.5% IGEPAL for 3-4 hours at 4 °C with GFP-trap resin (Chromotek). Beads were washed 3 times with the same buffer and bound proteins were eluted by addition of SDS loading dye and heating to 90°C for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The lysates were collected by centrifuging at 16000 g for 10 minutes and incubated with 50 μl of Myc-Trap®_A beads (Chromotek #yta-20, previously washed as before) for 2 hours at 4°C with rotation ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining supernatants were mixed with GFP-Trap magnetic beads (8 µL of slurry per sample; Chromotek, gtma-20) prewashed with IP Buffer [500 mM NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... and serine 2 (Chromotek, diluted 1:200). After washing with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 450 μl of lysate was added to 8 μl (slurry volume) of washed GFP-Trap agarose beads (GTA-20, Chromotek GmbH, Germany). The tubes were incubated on an end-over-end rotator at 4°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated at 4°C overnight with GFP-Trap agarose (Chromotek) or Dynabeads (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were immunoprecipitated at 4°C in lysis buffer with GFP-Trap (ChromoTek) beads as described by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Complexes were immunoprecipitated at 4°C in lysis buffer with GFP-Trap (ChromoTek) and washed in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Myc-Trap agarose beads A (Chromotek, yta-2 Myc Trap_A) were prepared by 3 rounds of washing in extraction buffer and centrifugation at 1000 x g for 5min at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Complexes were immunoprecipitated for 90 minutes at 4°C with GFP-Trap beads (Chromotek). After five washes in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... 4°C) and the supernatant incubated with 25 μl of equilibrated GFP-Trap resin (ChromoTek) for 2 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed before incubated with GFP-Trap Agarose beads overnight at 4 °C (Chromotek) and washed as manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... were immunoprecipitated from 2 mg of lysates using GFP-Trap beads (Chromotek) at 4°C for 4h under rotation ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed on a rotating wheel at 4°C overnight with GFP-Trap beads (Chromotek). The beads were then washed 4 times with lysis buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was incubated at 4°C for 1 h with GFP-Trap Agarose beads (Chromotek) and washed 4 times ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibody (mouse anti-mNeonGreen [32F6], Chromotek, 1:250) diluted in PBT + 1x WBR ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were cleared by centrifugation (6,000 g, 30 min, 4°C) and incubated with GFP-Trap (Chromotek) for 2h ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates incubated on a nutator at 4°C for two hours with 20μl of GFP-Trap beads (ChromoTek). GFP-Trap complexes were then washed four times in IP buffer and re-suspended in 2× SDS-PAGE sample buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Microbiology 2019Quote: ... and then diluted 1/4 in dilution buffer with 0.025% Tween-20 and added to washed GFP-Trap_MA beads (Chromotek). For the negative control ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysate was cleared by centrifugation and incubated for 2h at 4°C with magnetic GFP-Trap agarose (Chromotek). The beads were washed 3 x 10 min with Buffer 3 and were either used for mass spectrometry analysis or the proteins were eluted in 1× SDS buffer with 5 min incubation at 95°C for western blotting ...
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bioRxiv - Biochemistry 2022Quote: ... cleared lysate was incubated with 2 μl washed GFP-Trap magnetic agarose beads (Chromotek) per well for >4 h at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... S2Lb-GFP proteins were immunoprecipitated for 2 hours using a GFP-trap system (Chromotek) coupled to magnetic beads ...