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Citations for ChromoTek :
1 - 50 of 64 citations for 6 cyano 5 methoxy 12 methylindolo 2 3 a carbazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... rat antibodies for the detection of phosphorylated RNAPII (serine 5 and serine 2) (Chromotek, 1: 100) and secondary goat anti-rat Alexa Fluor 488 antibodies (A-11006 ...
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bioRxiv - Biochemistry 2020Quote: ... 12-20 μl of GFP-TRAP MA beads (Chromotek) were added to the supernatant and the mixtures were rotated for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... glycerol 5% and incubated for 2 h with 30 μl of pre-washed GFP-Trap magnetic agarose beads (gtma-20, ChromoTek, Planegg-Martinsried) using gentle rotation at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 6 µl GFP-Trap magnetic agarose (ChromoTek, gtmak-20) pre-equilibrated with dilution buffer was added to the diluted lysates ...
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bioRxiv - Cancer Biology 2020Quote: MDA-MB-231 cells with Tks5αGFP were cultured on HDFC prepared on #1.5 coverglass for 2 days before stained for F-actin (phalloidin-Alexa Fluor-647) and Tks5αGFP (GFP-nanobody, ChromoTek GFP Vhh, # gt-250) using manufacturer recommended procedures ...
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bioRxiv - Plant Biology 2022Quote: ... 5 μL washed Myc magnetic beads (Chromotek) were added to the protein extracts of each sample and samples were then incubated on a rolling wheel at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... and serine 2 (Chromotek, diluted 1:200). After washing with PBS ...
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bioRxiv - Microbiology 2021Quote: ... The resin was then washed 3 times in GFP-Trap_A Wash buffer (Chromotek) and GFP-proteins were eluted using SDS sample buffer at 95°C for 10 min and analyzed by immunoblotting.
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bioRxiv - Biochemistry 2023Quote: ... lysates were incubated with 5 µL Myc Trap Agarose (Chromotek) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... or for 5 h with GFP-Trap magnetic agarose (Chromotek) at 4°C under rotation ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant was incubated with 5 μl of myc-Trap beads (Chromotek) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Myc-Trap agarose beads A (Chromotek, yta-2 Myc Trap_A) were prepared by 3 rounds of washing in extraction buffer and centrifugation at 1000 x g for 5min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... SPD-5 was then bound to 500 μL MBP-trap beads (Chromotek) and the column was washed 3X with buffer 4 (25mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... SPD-5 was then bound to 500 μL MBP-trap beads (Chromotek) and the column was washed 3X with buffer 4 (25mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then transferred in 900 μl aliquots to fresh Eppendorf tubes and incubated with gentle head-over-tail agitation for 2 h at 4°C with 30 μl of anti-RFP or control beads (Chromotek rtma-2 and bmab-20, respectively) that had been washed in 1x lysis buffer.
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bioRxiv - Cell Biology 2023Quote: ... 100 mM Tris pH 7.5 and 5 µl of equilibrated GFP-Trap beads (Chromotek) and incubated overnight at 4°C with constant mixing ...
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bioRxiv - Biochemistry 2019Quote: ... were immunoprecipitated from 2 mg of lysates using GFP-Trap beads (Chromotek) at 4°C for 4h under rotation ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed in the same buffer with 0.5% IGEPAL for 3-4 hours at 4 °C with GFP-trap resin (Chromotek). Beads were washed 3 times with the same buffer and bound proteins were eluted by addition of SDS loading dye and heating to 90°C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were washed extensively and GFP-booster (tagged with Alexa488, ChromoTek, gb2AF488-50, 1:500 in 3%BSA/PBS) and anti-rabbit-Alexa568 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... 0.25 mM PMSF) and eluted with 4 × 1 mL 0.15 mg/mL (50 µM) 3 x FLAG peptide (Chromotek). The elutant was concentrated with Amico-4 (50k MWCO ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated with 5% (w/v) milk powder-TBST including anti-GFP (Chromotek) as primary antibody (diluted to1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... precleared cell extracts (10,000, 5 min) and incubated the supernatants with Myc-trap resin (Chromotek) for 3 hours at 4 °C.
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bioRxiv - Biochemistry 2021Quote: ... Cells were blocked in 3% BSA in PBS-T for 30 min before incubation with GFP-Booster _Atto488 (1:300; Chromotek), or mouse anti-G3BP1 in 3% BSA in PBS-T for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... The immunoprecipitation was performed using 3 mL of plant extract mixed with 25 µL of GFP-trap magnetic beads (Chromotek) at 4 °C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were cleared by 10 min centrifugation at 12,000 rpm and the supernatant was incubated on a rotor wheel at 4°C for 3 h with 25 µl of GFP-Trap magnetic agarose bead slurry (Chromotek), pre-washed with extraction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... cleared lysate was incubated with 2 μl washed GFP-Trap magnetic agarose beads (Chromotek) per well for >4 h at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... S2Lb-GFP proteins were immunoprecipitated for 2 hours using a GFP-trap system (Chromotek) coupled to magnetic beads ...
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bioRxiv - Microbiology 2021Quote: ... Slides were subsequently blocked overnight in PBS containing 3% BSA before labelling with anti-mNeonGreen mouse monoclonal antibody (32F6; 1:300; Chromotek, Germany) followed by Alexa Fluor 488 goat anti-mouse antibody (1:1000 ...
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bioRxiv - Systems Biology 2020Quote: ... were proportioned for analysis of protein expression (5%) and immunoprecipitation (95%) using GFP-Trap beads (ChromoTek, Germany). Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 5 in CTD domain rat 3E8 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... Immunocytochemistry was performed with the following primary antibodies: rat anti-GFP (Chromotek, 50430-2-AP) 1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Lysates from 21-GFP cells grown on two 150 mm dishes and infected with HSV-2 strain 186 at an MOI of 3 or uninfected were prepared at 18 hpi and immobilized on GFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... For the localization of RNAPII we used antibodies recognizing RNAPII phosphorylated at serine 5 (Chromotek, diluted 1:200) and serine 2 (Chromotek ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were incubated (2 h, at 4°C) with 20 µl GFP-Trap®_MA (ChromoTek). Beads were then washed 3 times for 10 min with 1 ml of IP buffer and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 4 mgs of protein extract per sample was then incubated with GFP-Traps beads (ChromoTek; 3 μl/mg of extract) for 3 hours at 4 °C ...
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bioRxiv - Genomics 2019Quote: ... Cell lysates were cleared by centrifugation at 5,000 g for 5 min before incubation with 50 µl pre-washed GFP-TRAP MA beads (Chromotek) for 1 h at 4 degrees Centigrade ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on 20 μl GFP-Trap beads (ChromoTek) for 30 min at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 2 in CTD domain rat 3E10 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Immunology 2023Quote: ... 2 mg of cell lysates were incubated with 25 ml of GFP-TRAP beads (Chromotek, GTA-20) for 1 hr at 4°C on rotation ...
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bioRxiv - Microbiology 2020Quote: ... GFP-nsP2 or nsP2-2EGFP translated from the replicon RNA were immunoprecipitated with 5 µl GFP-Trap magnetic agarose beads (Chromotek) at 4°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were incubated with a 5-μL slurry of anti-Spot nanobody-coupled magnetic agarose beads (Spot-Trap, ChromoTek) for 3 h at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were incubated for 2 hours at 4°C with 25 μL GFP-Trap coupled agarose beads (Chromotek). After incubation ...
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bioRxiv - Genomics 2020Quote: ... at 4°C or for 2 hours with 20 uL of GFP-Trap magnetic beads (Chromotek, gtma-20) at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a 1:1,000 dilution of mouse anti-GFP [B-2] (SantaCruz Biotechnology) or a rat anti-RFP [5F8] (Chromotek) followed by a 1:5,000 dilution of the horseradish peroxidase-conjugated secondary antibody against mouse IgG (7076 ...
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bioRxiv - Cell Biology 2020Quote: ... For IP 2 mg of cytoplasmic and nuclear extracts were incubated with either 15 µl GFP Trap beads (Chromotek) for 1 h at 4°C or 1-1.5 μg IgG overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...