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Citations for ChromoTek :
101 - 124 of 124 citations for 6 Hydroxy 4 trifluoromethyl 2 3' bipyridine 5 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 2 mg of cell lysates were incubated with 25 ml of GFP-TRAP beads (Chromotek, GTA-20) for 1 hr at 4°C on rotation ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 20000 × g for 10 min at 4°C and added to pre-equilibrated beads (Chromotek, GTA-100) and rotated for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated after centrifugation (13000x g for 10 min at 4°C) and incubated with 25 μl GFP Trap (Chromotek, gtm-20) magnetic beads for 1 hour at 4°C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Developmental Biology 2021Quote: ... two different protocols were used: 1) one step of O/N incubation at 4°C with GFP booster (1:250, Alexa Fluor ® 488 Chromotek #gb2AF488), Phalloidin-647 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 50 µL of sepharose beads for 1 h at 4°C with constant rotation and subjected to immunoprecipitation with 25 µL GFP Trap agarose beads (Chromotek, gta-20) during 1 h at 4°C with constant rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 200 µL of sepharose beads for 1 h at 4°C under constant rotation and then subjected to immunoprecipitation with 50 µL of GFP Trap agarose beads (Chromotek, gta-20) for 2 h at 4°C under constant rotation ...
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bioRxiv - Microbiology 2020Quote: ... GFP-nsP2 or nsP2-2EGFP translated from the replicon RNA were immunoprecipitated with 5 µl GFP-Trap magnetic agarose beads (Chromotek) at 4°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were incubated with a 5-μL slurry of anti-Spot nanobody-coupled magnetic agarose beads (Spot-Trap, ChromoTek) for 3 h at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a 1:1,000 dilution of mouse anti-GFP [B-2] (SantaCruz Biotechnology) or a rat anti-RFP [5F8] (Chromotek) followed by a 1:5,000 dilution of the horseradish peroxidase-conjugated secondary antibody against mouse IgG (7076 ...
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bioRxiv - Cell Biology 2020Quote: ... For IP 2 mg of cytoplasmic and nuclear extracts were incubated with either 15 µl GFP Trap beads (Chromotek) for 1 h at 4°C or 1-1.5 μg IgG overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Cell Biology 2023Quote: ... Insoluble material was removed by centrifugation at 14,000 x g for 2 minutes and the supernatant was incubated with 50 µl HNG-washed agarose beads (Chromotek) with end-over-end rotation for 15 minutes to capture non-specific binding proteins ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then probed overnight at 4°C with TBST buffer containing 1% non-fat dry milk and primary antibodies: mouse monoclonal anti-RFP (Chromotek, clone 6G6, 1:3,000), rabbit polyclonal anti-GFP (SYSY ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 mM dithiobis [succinimidyl propionate] containing 1x Complete Protease Inhibitor Cocktail) and then incubated with GFP-TRAP agarose beads (ChromoTek) for 2 hours at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... A volume of 2 mL of total protein was mixed with 25 μL of GFP-TRAP agarose beads (50% slurry, ChromoTek) and incubated shaking overnight at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The GFP-TRAP agarose beads were centrifuged for 2 min at 3000 x g before washing three times with wash buffer (ChromoTek). Bound proteins were recovered by resuspending the beads in loading buffer (loading dye ...
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bioRxiv - Cell Biology 2022Quote: ... transduced cells were incubated with 100 μL Hybridization Buffer containing 2 μL 12.5 μM CF568-labled gRNA FISH probes and 1:2000 AF647-labeled anti-GFP nanobody (Chromotek, gb2AF647-50) for 4 hours in the dark ...
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bioRxiv - Pathology 2020Quote: ... aliquots of the supernatants equivalent to 2 x 108 cells were incubated with 10 μL anti-Myc agarose beads (Myc-Trap™, Chromotek) for 1 h at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 500μg of lysates in 500 μL of SEC buffer were incubated for 2 hours with GFP-trap magnetic agarose beads (Chromotek #gtma-10) or binding control beads (Chromotek #bmab-20) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with phalloidin diluted in 2% BSA/PBS at room temperature for 20 min or stained with phalloidin and GFP-booster (ChromoTek, gb2AF488-10) when imaging cortactin-EGFP using 3D-SIM ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of the protein lysates was incubated for 2 h with 10 µl of either Myc-Trap® or GFP-Trap® beads (Chromotek). To determine the dependence of interactions on RNA ...