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Citations for ChromoTek :
51 - 100 of 117 citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and then diluted 1/4 in dilution buffer with 0.025% Tween-20 and added to washed GFP-Trap_MA beads (Chromotek). For the negative control ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysate was cleared by centrifugation and incubated for 2h at 4°C with magnetic GFP-Trap agarose (Chromotek). The beads were washed 3 x 10 min with Buffer 3 and were either used for mass spectrometry analysis or the proteins were eluted in 1× SDS buffer with 5 min incubation at 95°C for western blotting ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 1h at 4 °C before being added to 10 µl magnetic V5 beads (v5tma, Chromotek), pre-equilibrated in buffer K ...
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bioRxiv - Biochemistry 2022Quote: ... cleared lysate was incubated with 2 μl washed GFP-Trap magnetic agarose beads (Chromotek) per well for >4 h at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... S2Lb-GFP proteins were immunoprecipitated for 2 hours using a GFP-trap system (Chromotek) coupled to magnetic beads ...
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bioRxiv - Systems Biology 2020Quote: ... were proportioned for analysis of protein expression (5%) and immunoprecipitation (95%) using GFP-Trap beads (ChromoTek, Germany). Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 5 in CTD domain rat 3E8 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... EGFP-INPP5E or its mutants were then immunoprecipitated from 1 mg of cell lysate by overnight rotation at 4°C with GFP-Trap_MA beads (Chromotek). Beads were then washed thrice in buffer containing 50mM Tris-HCl pH=7.5 and 150mM NaCl buffer (no protease inhibitors or detergent) ...
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bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 200 μl of GFP-Trap_MA magnetic beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... for 30 min at 4°C and then with GFP-Trap Magnetic Particles M-270 (gtd-20, ChromoTek GmbH) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were then incubated for 1.5 h at 4 ⁰C with 25 µL RFP-Trap agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Biophysics 2023Quote: ... 0.25 mM PMSF) and eluted with 4 × 1 mL 0.15 mg/mL (50 µM) 3 x FLAG peptide (Chromotek). The elutant was concentrated with Amico-4 (50k MWCO ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was cleared at 14,000 rpm for 10 min and incubated for 10 min rotating at 4 °C together with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek). Beads were washed four times with GFP pull-down buffer at 2,500 g for 2 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at +4°C with the primary antibody (rabbit anti-GFP, 1:600, Chromotek) diluted in the blocking solution ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were centrifuged at 21,000 g at 4 °C for 10 min and then incubated for 10 min at 4 °C with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek) rotating ...
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bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by filtering through miracloth followed by centrifugation (6,000 g, 30 min, 4°C) and then incubated with GFP-Trap (Chromotek) for 4h ...
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bioRxiv - Neuroscience 2024Quote: ... Immunocytochemistry was performed with the following primary antibodies: rat anti-GFP (Chromotek, 50430-2-AP) 1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... For the localization of RNAPII we used antibodies recognizing RNAPII phosphorylated at serine 5 (Chromotek, diluted 1:200) and serine 2 (Chromotek ...
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bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... samples were spun down 10 min at 14000 rpm and 4°C and supernatant was added to GFP-Trap agarose beads from Chromotek and incubated for 1.5 hours at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were collected (centrifugation for 10 min at 21,100 g and 4 °C) and the supernatant was added to 25 µl of blocked GFP-Trap Agarose (gta, ChromoTek).
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bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 40 μl of GFP-Trap MA magnetic Beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
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bioRxiv - Plant Biology 2020Quote: ... 50μg of total proteins were incubated 3H at 4°C with 30μl of GFP-Trap Agarose beads (Chromotek; gta-20) under gentle agitation for GFP-tagged protein purification ...
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bioRxiv - Plant Biology 2020Quote: ... The blot was incubated overnight on a shaker (4°C) with the primary antibody (anti-GFP 3H9, anti-RFP 6G6 (Chromotek) or anti-tRFP (Evrogen)) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was incubated for 1 h at 4 °C with GFP-Selector beads (NanoTag) (Fig. 4A, B and D) or with Myc-trap beads (Chromotek) (Fig ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were cleared by 10 min centrifugation at 12,000 rpm and the supernatant was incubated on a rotor wheel at 4°C for 3 h with 25 µl of GFP-Trap magnetic agarose bead slurry (Chromotek), pre-washed with extraction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... on a rotating wheel at 4° C for 1 h in triplicates with GFP-Trap agarose beads (#gta-200, Chromotek) or control agarose beads (Chromotek) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were cleared by centrifugation for 30 min at 4°C and incubated with GFP-trap magnetic agarose beads (Chromotek) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatant was cleared at 14000 rpm for 10 minutes and incubated for 30 minutes rotating at 4 °C together with 25 µl pre-equilibrated Spot-Trap beads (Chromotek). Beads were washed 4 times with GFP PD buffer at 2500 g for 2 minutes at 4 ° C ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 4°C and added to GFP-Trap Magnetic agarose beads (Chromotek, cat #gtma-10) for 1 hr at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged for 20 min (full speed, 4°C) and the supernatant was incubated with mEGFP-Trap Agarose beads (ChromoTek) for 1 hour according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysate was centrifuged at 17,000 g for 10 min at 4 °C and supernatant was applied to 20 μl of GFP-Trap beads (Chromotek) and shaken for 2 hr at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The insoluble material was removed by centrifugation and the lysates were subjected to immunoprecipitation either by subsequent incubations with anti-Ecad antibody SHE78-7 (4 μg/ml) and protein A-beads or by incubation with GFP-trap beads (Chromotek). After incubation ...
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bioRxiv - Microbiology 2019Quote: ... and 200 ng total membrane proteins were incubated for 1 h at 4°C with gentle shaking on a roller mix with either 25 µl GFP-Trap® agarose beads (Chromotek) in a final volume of 100 µl 1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... The rest of the supernatants were incubated for 15 min at 4°C with 10 μl of GFP-Trap® Agarose beads (gta-10; Chromotek) to remove free GFP and spun at 1200 xg for 1 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Cytoplasmic and nuclear fractions were incubated for 30 minutes at 4°C under agitation with 120 µL of control agarose beads (ChromoTek, Germany). Pre-cleared lysates were collected by a centrifuging 2 min at 400g (4°C) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting protein extracts were centrifuged at maximum speed on a table-top centrifuge for 15 minutes at 4 °C and the supernatant (soluble fraction) incubated with 25 μl GFP-trap magnetic beads (ChromoTek, gtma) for four hours at 4 °C under constant rotation ...
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bioRxiv - Genomics 2019Quote: ... Cell lysates were cleared by centrifugation at 5,000 g for 5 min before incubation with 50 µl pre-washed GFP-TRAP MA beads (Chromotek) for 1 h at 4 degrees Centigrade ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 2 in CTD domain rat 3E10 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Immunology 2023Quote: ... 2 mg of cell lysates were incubated with 25 ml of GFP-TRAP beads (Chromotek, GTA-20) for 1 hr at 4°C on rotation ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 20000 × g for 10 min at 4°C and added to pre-equilibrated beads (Chromotek, GTA-100) and rotated for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated after centrifugation (13000x g for 10 min at 4°C) and incubated with 25 μl GFP Trap (Chromotek, gtm-20) magnetic beads for 1 hour at 4°C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Developmental Biology 2021Quote: ... two different protocols were used: 1) one step of O/N incubation at 4°C with GFP booster (1:250, Alexa Fluor ® 488 Chromotek #gb2AF488), Phalloidin-647 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 50 µL of sepharose beads for 1 h at 4°C with constant rotation and subjected to immunoprecipitation with 25 µL GFP Trap agarose beads (Chromotek, gta-20) during 1 h at 4°C with constant rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 200 µL of sepharose beads for 1 h at 4°C under constant rotation and then subjected to immunoprecipitation with 50 µL of GFP Trap agarose beads (Chromotek, gta-20) for 2 h at 4°C under constant rotation ...