Labshake search
Citations for ChromoTek :
351 - 400 of 420 citations for Rabbit Anti Leptospira Polyclonal Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated with 5% (w/v) milk powder-TBST including anti-GFP (Chromotek) as primary antibody (diluted to1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: eYFP-tagged fusion proteins were immunoprecipitated using anti-GFP magnetic agarose beads (Chromotek, gtma-20). After induction of gene expression of lentiviral transduced KO2 cells with 1 µg/ml doxycycline ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-RFP (Invitrogen, #R10367, 1:1000 for WB and Chromotek #5F8, 1:1000 for WB), anti-phospho myosin light chain (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2022Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: Affinity purification was performed using anti-GFP nanobody coupled magnetic beads (∼ 30µl slurry) (Chromotek AB_2631358). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... The precleared lysate was incubated with an anti-GFP-Trap-A beads (ChromoTek, gta-20) antibody overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... the RFP-Booster Alexa Fluor 568 (alpaca monoclonal anti-RFP Nanobody; 1:1000; rb2AF568, ChromoTek) was used with second antibodies to amplify the signal of NLS-sfCherry.
-
bioRxiv - Microbiology 2020Quote: ... GFP-Trap Magnetic Agarose beads or control beads without antibodies were used according to the manufactured protocol (both Chromotek, Planegg-Martinsried, Germany). The loaded magnetic beads were heated for 10 min at 95 °C in SDS loading buffer for the dissociation of purified proteins ...
-
bioRxiv - Microbiology 2019Quote: ... The nanoboosters - single-domain alpaca antibody fragments covalently coupled to fluorescent dyes — GFP-Booster Atto488 and RFP-Booster Atto594 (Chromotek, Martinsried, Germany) — were used to boost eYFP and mCherry fluorescence ...
-
bioRxiv - Microbiology 2021Quote: ... Protein extracts were then subjected to immunoprecipitation using agarose beads coupled with anti-RFP nanobodies (Chromotek). Eluates were collected and analysed by western blot ...
-
bioRxiv - Plant Biology 2021Quote: ... Anti-GFP coated beads were added (25 μL ChromoTek GFP-Trap or 50 μL MACS magnetic) and IP was carried out as per the manufacturers’ instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... each supernatant was incubated with 50 µL of Anti-cMyc agarose beads (Chromotek, 004282-07-02) at 4°C for 4 h ...
-
bioRxiv - Plant Biology 2023Quote: ... The supernatant was subjected to immunoprecipitation for 1 hour using anti-GFP (ChromoTek, Planegg- Martinsried Germany), anti-RFP (ChromoTek ...
-
bioRxiv - Plant Biology 2023Quote: ... 600 µL of plant lysate was incubated with 30 µL of anti-mRFP magnetic beads (Chromotek) in a rotary mixer for 1 hrs at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... The rest of the supernatant was mixed with 8 µL anti-GFP beads (Chromotek, gta-20). The mixture was incubated for 2 h at 4 °C while rotating ...
-
bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
-
bioRxiv - Microbiology 2021Quote: ... cells were fixed and treated for immunofluorescence with α-SINV C antibody at 1:200 dilution and GFP-Booster_Atto488 (ChromoTek GmbH, #gba488-100) at 1:500 dilution following the procedure described in (Garcia-Moreno et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
-
bioRxiv - Microbiology 2024Quote: ... Secondary antibody incubation was carried out for 1 hour at room temperature with GFP-Booster Alexa Fluor 488 (Chromotek, gb2AF488, 1:500 dilution) and Alexa Fluor 647 donkey anti-rabbit IgG (ThermoFisher ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labeled anti-GFP nanobodies (Chromotek) for 30 min ...
-
bioRxiv - Plant Biology 2022Quote: ... Twenty μl of anti-GFP beads was mixed with 1.4 ml of filtered extracts (Chromotek, Munich, Germany) and incubated at 4□ for 2 hours ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were further incubated on ice with membrane impermeable Alexa Fluor 647– labelled anti-GFP nanobodies (Chromotek) for 30 min ...
-
bioRxiv - Cell Biology 2021Quote: ... cleared lysates containing GFP-tagged proteins were added to anti-GFP agarose beads (GFP-Trap, ChromoTek, Germany) and incubated while rotating end over end for an hour at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: Expressed GFP-ALDH9A1 was enriched using anti-GFP beads following the manufacturer’s protocol for GFP-Trap (Chromotek). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: ... then incubated overnight on a Nutator with anti-GFP bound agarose beads (Chromotek GFP-Trap® #gta). Lysates and beads were separated in a centrifuge ...
-
bioRxiv - Cell Biology 2023Quote: ... The signal of mCherry and GFP was enhanced using a rat mAb anti-RFP (Chromotek, 5f8-100) and a rabbit pAb anti-GFP antibody (BIOZOL/MBL ...
-
bioRxiv - Plant Biology 2019Quote: ... (2009) using for BPC1-RFP an anti-RFP VHH coupled to magnetic agarose beads RFP-trap_MA® (Chromotek). Real-time PCR assays were performed to determine the enrichment of the fragments ...
-
bioRxiv - Biochemistry 2021Quote: ... immunoprecipitation was performed using either GFP-Trap (high affinity anti-GFP alpaca nanobody bound to agarose beads; Chromotek) or anti-HA beads (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2019Quote: ... diluted with 300 µl buffer and loaded on magnetic agarose anti-GFP beads (GFP-trap®_MA, ChromoTek) for 1 h at 4°C ...
-
bioRxiv - Immunology 2023Quote: The input and elution samples obtained after immunoprecipitation using anti-GFP beads (Miltenyi or agarose beads from Chromotek). If necessary ...
-
bioRxiv - Cell Biology 2020Quote: ... Co-IP’s on the total cell lysates or on subcellular fractions (nuclear and cytoplasmic) were performed at different timepoints using anti-GFP VHH coupled to magnetic agarose beads (GFP-Trap_MA, Chromotek) or ...
-
bioRxiv - Plant Biology 2019Quote: ... The homogenate was then centrifuged twice at 10,000 rpm for 10 min and incubated with 20 ul of anti-mCherry Trap agarose beads (Chromotek) for 12-16 h at 4°C on a rocking shaker ...
-
bioRxiv - Neuroscience 2020Quote: ... 2 and 3 GAGs fusion proteins were first concentrated by immunoprecipitation of roughly 20,000 nematodes per genotype using Trap-A bead coupled with anti-mNG nanobody (Chromotek). 1.5 U of heparanase I and III (Sigma ...
-
bioRxiv - Cancer Biology 2021Quote: ... The precleared lysate was incubated with 30 μl of magnetic agarose beads coupled to anti-GFP nanobodies (GFPTrap; ChromoTek) for 1 hr at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: Single-molecule tracking was carried out in modified Tyrode’s solution supplement with 0.8% BSA and ATTO647N-conjugated anti-GFP nanobodies (1:15000 dilution, GFPBooster-ATTO647N, Chromotek, #gba647n) on Nanoimager microscope (Oxford Nanoimaging ...
-
bioRxiv - Developmental Biology 2021Quote: ... blocked as above and incubated in primary (rat anti-GFP, 1:200, ChromoTek, Cat. No. 3h9-100, clone 3H9) diluted in block as above ...
-
bioRxiv - Plant Biology 2021Quote: ... The membrane was blocked using BlueBlock PF (SERVA) and developed using rat monoclonal anti-RFP 5F8 (1:2000, Chromotek) and rabbit anti-rat-peroxidase A9542 (1:20000 ...
-
bioRxiv - Plant Biology 2022Quote: ... The ribosylated NbFBN1a2A-GFP and NbFBN1a-GFP were detected by the anti-pan-ADP-ribose binding reagent after they were pulled down with Co-IP by GFP-Trap_A (ChromoTek).
-
bioRxiv - Microbiology 2020Quote: ... and then incubated with 25 μl anti-HA magnetic beads (Pierce) or with 25 μL GFP-Trap Magnetic Agarose (Chromotek) overnight at 4 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
-
bioRxiv - Plant Biology 2020Quote: ... Small cuts from the infiltrated regions were incubated for one hour in anti-Spot-Tag VHH/Nanobodies conjugated to the organic fluorophore ATTO488 (Chromotek), diluted 1:1000 in 10 mM Tris-HCl solution pH 7.5 ...
-
bioRxiv - Plant Biology 2020Quote: ... The roots were incubated overnight at 4°C with the secondary anti-mouse (1:500; Jackson immunoreactive, conjugated to Rhodamine Red) and eba488-10 Spot Label ATTO488 (1:1000, Chromotek) diluted in PBS supplemented with 2% (w/v ...
-
bioRxiv - Molecular Biology 2020Quote: ... then mixtures were centrifuged (15 min, 4º, 14000 rcf) and the supernatant (input) was added to washed anti-GFP beads (Chromotek). Beads and lysates were incubated 2-4 h at 4 °C while rotating ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek, Germany, Cat. No. gtma-20) for 3h at 4°C while rotating ...
-
bioRxiv - Cancer Biology 2019Quote: ... The following nanobodies and dyes were used for whole body immunostaining: Atto647N conjugated anti-RFP/mCherry nanobooster (Chromotek, rba647n-100), Atto594 conjugated anti-RFP/mCherry nanobooster (Chromotek ...
-
bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
-
bioRxiv - Developmental Biology 2021Quote: ... Vibratome sections prepared from inflated and fixed lobes at 350 µm thickness were stained with chicken anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lungs were collected at 5 days after tamoxifen injection and vibratome sections (350 µm) were stained with rat anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
-
bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was then aspirated without disturbing the upper lipid layer and split by half into either the anti-GFP agarose beads or the control blocked beads (22 μl, Chromotek). After head over tail rotation at 4°C for 90 min ...