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Citations for ChromoTek :
301 - 350 of 350 citations for Rat Butyrophilin Like Protein 2 BTNL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Then 800-1000 µg of total protein lysates were added to 25 μl of anti-GFP magnetic beads (ChromoTek GFP-Trap) and tumbled end-over-end for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: For CoIP experiments 500 μl protein extract was mixed with 25 μl calibrated anti-GFP magnetic beads (ChromoTek GFP-Trap®) and incubated on a rotator for 2 h ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Plant Biology 2020Quote: Blotted proteins were detected using anti-GFP (LifeTechnologies, Darmstadt, Germany) and anti-red FP (RFP) antibody (ChromoTek GmbH, Planegg-Martinsried, Germany) (both ...
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bioRxiv - Cell Biology 2023Quote: ... and approximately 250 µg of protein was incubated with anti-GFP-conjugated magnetic agarose beads (ChromoTek GFP-Trap® Magnetic Agarose) for 1-1.5 hours ...
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bioRxiv - Plant Biology 2024Quote: ... between EIN2-C and HAF2 following study previous briefly: Total protein extracts from plants mentioned in the paper were immunoprecipitated by DYKDDDDK Fab-Trap™ agarose beads (ChromoTek) for two hours at 4 °C with gentle rotating and then beads were washed five times with ice-cold 1XPBS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... For IP 2 mg of cytoplasmic and nuclear extracts were incubated with either 15 µl GFP Trap beads (Chromotek) for 1 h at 4°C or 1-1.5 μg IgG overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Insoluble material was removed by centrifugation at 14,000 x g for 2 minutes and the supernatant was incubated with 50 µl HNG-washed agarose beads (Chromotek) with end-over-end rotation for 15 minutes to capture non-specific binding proteins ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Microbiology 2020Quote: ... Twenty milligram protein extracts were taken as input and the rest of the extracts were incubated with the GFP-Trap beads (ChromoTek, gta-20) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates (200 μg protein) from HEK-293T cells expressing WT-TRPC6-YFP was incubated with GFP M-trap beads (Chromotek GTD-20) to immunoprecipitate TRPC6 ...
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bioRxiv - Plant Biology 2019Quote: ... The protoplasts were collected and the subsequent work was operated following the protocol for immunoprecipitation of Myc-fusion proteins using Myc-Trap®_MA (Chromotek, Germany). Both the input and the IP samples were separated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mL of protein extract was incubated with 60 μL (50% slurry) of GFP-trap magnetic agarose beads (ChromoTek, Planegg-Martinsried, Germany) for 3 h on a rotary shaker at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... One third of the whole cell lysate was pre-cleared with a 50:50 mixture of protein A/G magnetic beads and GFP-Trap (Chromotek, gtd-20) was pre-blocked with 1mg/mL ultra-pure BSA (AM2616 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 mM dithiobis [succinimidyl propionate] containing 1x Complete Protease Inhibitor Cocktail) and then incubated with GFP-TRAP agarose beads (ChromoTek) for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 15 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 150 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mL of supernatant was incubated at 4°C for 2 h with GFP-Trap_A beads (Chromotek, Planegg-Martinsried, Germany). The beads were collected by centrifugation at 800 × g and washed four times in 1 mL washing buffer (25 mM Tris pH=7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... The GFP-TRAP agarose beads were centrifuged for 2 min at 3000 x g before washing three times with wash buffer (ChromoTek). Bound proteins were recovered by resuspending the beads in loading buffer (loading dye ...
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bioRxiv - Microbiology 2020Quote: ... The GFP-CchA were enriched with a GFP-Trap kit (ChromoTek). After enrichment ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitation was carried out using the GFP-Trap Agarose KIT (ChromoTek), following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Cancer Biology 2023Quote: We used the PARP1-Trap Agarose kit [xtak-20] from Chromotek which consists of a PARP1 Nanobody/ VHH ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 4 mgs of protein extract per sample was then incubated with GFP-Traps beads (ChromoTek; 3 μl/mg of extract) for 3 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves and pulldown assay were carried out according to102 with the following modifications: For immunoprecipitation of GFP-tagged proteins 50 µl of GFP-Trap magnetic beads (Chromotek, Planegg-Martinsried, Germany) were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™ ...
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bioRxiv - Cell Biology 2022Quote: ... transduced cells were incubated with 100 μL Hybridization Buffer containing 2 μL 12.5 μM CF568-labled gRNA FISH probes and 1:2000 AF647-labeled anti-GFP nanobody (Chromotek, gb2AF647-50) for 4 hours in the dark ...
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bioRxiv - Pathology 2020Quote: ... aliquots of the supernatants equivalent to 2 x 108 cells were incubated with 10 μL anti-Myc agarose beads (Myc-Trap™, Chromotek) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... The cultures were harvested and total protein samples were extracted from vegetative mycelia and incubated with anti-GFP beads (GFP-Trap®_A, Chromotek, Hauppauge, NY, USA) 90 minutes at 4°C with gentle shaking following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... and the protein extract was separated on reducing SDS-PAGE and probed with commercial anti-RFP or anti-GFP antibodies (6G6, Chromotek or ab290, Abcam, respectively) to detect mCherry tagged MSBP1 or GFP-tagged ATI1 ...
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bioRxiv - Microbiology 2021Quote: ... GFP-EhPTEN1 was immunoprecipitated using GFP-Trap Agarose Kit (ChromoTek, Planegg, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Co-immunoprecipitation was performed using a GFP-Trap Kit (Chromotek, #gtma-20) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 500μg of lysates in 500 μL of SEC buffer were incubated for 2 hours with GFP-trap magnetic agarose beads (Chromotek #gtma-10) or binding control beads (Chromotek #bmab-20) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with phalloidin diluted in 2% BSA/PBS at room temperature for 20 min or stained with phalloidin and GFP-booster (ChromoTek, gb2AF488-10) when imaging cortactin-EGFP using 3D-SIM ...
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bioRxiv - Neuroscience 2021Quote: ... glycerol 5% and incubated for 2 h with 30 μl of pre-washed GFP-Trap magnetic agarose beads (gtma-20, ChromoTek, Planegg-Martinsried) using gentle rotation at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: MDA-MB-231 cells with Tks5αGFP were cultured on HDFC prepared on #1.5 coverglass for 2 days before stained for F-actin (phalloidin-Alexa Fluor-647) and Tks5αGFP (GFP-nanobody, ChromoTek GFP Vhh, # gt-250) using manufacturer recommended procedures ...
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bioRxiv - Plant Biology 2022Quote: Immunoprecipitation was carried out using the GFP-trap® magnetic agarose kit (Chromotek, Germany) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Co-Immunoprecipitation (Co-IP) experiments were performed using the GFP-Trap®_M kit (ChromoTek). Proteins were extracted by grinding roots of 10-day-old Arabidopsis seedlings expressing pCAMV35S::GFP or pRXR1::RXR1-GFP in liquid N2 ...
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bioRxiv - Cell Biology 2022Quote: ... Co-immunoprecipitation was performed using a GFP- or RFP-Trap Kit (Chromotek, #gtma-20, #rtma-20) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Explant immunoprecipitation (IP) was then performed using a GFP-Trap Agarose Kit (ChromoTek, catalog number gtak-20) and proteins were eluted in 2X sample buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested two days later and ZAP was immunoprecipitated using GFP-Trap magnetic agarose kit (Chromotek) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The cultured explants were collected and immunoprecipitation (IP) was performed using GFP-Trap Agarose Kit (ChromoTek, cat# gtak-20). Immunoprecipitated proteins were eluted in 2X sample buffer ...
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bioRxiv - Molecular Biology 2021Quote: The SLO-treated trophozoite stage lysate of PfHDP-GFP transgenic parasites was immunoprecipitated using GFP-Trap®_A Kit (Chromotek)/ anti-GFP antibody following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Assay Kit (MilliporeSigma, Cat# 17-295) with adaptations based on the application notes on GFP- and RFP-Trap by ChromoTek. 100 Eye-antennal discs for each replicate were dissected in ice-cold 1xPBS ...
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bioRxiv - Plant Biology 2022Quote: Meiotic buds from ∼150 inflorescences from CDKD;3:YFP and wild type control were grinded in liquid nitrogen and processed using the ChromoTek GFP-Trap® Magnetic Agarose kit (Chromotek). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... Cell lysates were cleared and then incubated with GFP-Trap MA beads as instructed in ChromoTek GFP-Trap® MA immunoprecipitation kit (ChromoTek Inc., Hauppauge, NY). Cell lysates were subsequently washed twice in dilution buffer (10 mM Tris-HCl pH 7.5 ...