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Citations for ChromoTek :
251 - 300 of 343 citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 50μg of total proteins were incubated 3H at 4°C with 30μl of GFP-Trap Agarose beads (Chromotek; gta-20) under gentle agitation for GFP-tagged protein purification ...
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bioRxiv - Microbiology 2020Quote: ... equal concentration of total proteins was isolated and incubated with 20~30 μL of GFP-Trap agarose beads (ChromoTek, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining supernatants were used for immunoprecipitation of GFP-fused proteins agarose using GFP-Trap coupled to agarose beads (Chromotek) and following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted as indicated above and immunoprecipitation was performed with 15 μL of GFP-trap beads (ChromoTek, Germany) or ANTI-FLAG M2 Affinity Agarose Gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... Clover- and Cherry-tagged proteins were immunoprecipitated by incubation with either GFP-(gta) or RFP-Trap(rta) magnetic beads/agarose (Chromotek) for 1 h at 4°C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... the extract prepared from OSC lines expressing endogenously tagged proteins of interest was incubated with magnetic GFP-Trap agarose (Chromotek) for 3h at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and subjected to immunoprecipitation of RFP/mCherry/dsRed-tagged or GFP-tagged fusion proteins using RFP-trap or GFP-trap matrix (Chromotek), respectively ...
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bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mg of the total proteins were incubated with 20 μL of GFP-Trap magnetic agarose beads (GFP-TrapMA; Chromotek) for 2 hours at 4°C by vertical rotation ...
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bioRxiv - Plant Biology 2022Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
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bioRxiv - Plant Biology 2024Quote: ... A volume of 2 mL of total protein was mixed with 25 μL of GFP-TRAP agarose beads (50% slurry, ChromoTek) and incubated shaking overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 0.6 mL of the resulting supernatant (containing 2 – 4 mg of proteins) were supplemented with 15 μL of RFP-Trap magnetic particles (ChromoTek), and incubated for 2.5 h at 4°C on a rotating wheel ...
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bioRxiv - Biochemistry 2024Quote: Protein extracts containing GFP-tagged MIK2 or site-directed mutants were incubated with 20 µL of GFP-Trap beads (Chromotek) for 2 h with gentle mixing at 4 °C to immuno-precipitate receptor complexes ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins (GFP-Trap; ChromoTek GmbH, Germany; gta-20) was used for immunoprecipitation of mCerN1 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mL of the protein supernatant was incubated with 10 μL of magnetic GFP-Trap beads (Chromotek, Cat. No. gtma) or magnetic Myc-Trap beads (Chromotek ...
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bioRxiv - Developmental Biology 2023Quote: ... A maximum of 500 ug total protein in a volume of 950 ul was loaded per 25 ul GFP-Trap agarose beads (gta, Chromotek), prior washed with ice-cold PBS (2 min at 2’500 x g ...
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bioRxiv - Neuroscience 2024Quote: ... for 1h at 4 °C to absorb bead-binding nonspecific proteins and immunoprecipitated using 50 μl of RFP-Trap magnetic Agarose (rtma, ChromoTek) for another 1h at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... 600 μL of the supernatant were transferred to a 2.0 mL tube and an equal volume of protein extraction buffer with 5 μL GFP-Trap Magnetic Agarose beads (ChromoTek) were added ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mg of protein lysate was incubated overnight at 4°C with 75 µl of GFP-Trap Agarose (gta-20, ChromoTek). For co- immunoprecipitations for MHC-I ...
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bioRxiv - Plant Biology 2021Quote: ... The membranes were incubated with primary rat monoclonal anti-5F8 antibody (Chromotek, 1:1000) and secondary bovine anti-rat horseradish peroxidase (HRP)-conjugated (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... Sov antibodies bound to agarose for Sov co-immunoprecipitation or anti-GFP agarose (Chromotek) for sfGFPSprB (368 aa ...
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were used: ATTO-488 GFP- booster at 1:200 (Chromotek), Alexa-488 anti-Mouse at 1:500 (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were incubated with the following antibodies: mouse α-mNeonGreen (1/500; 32F6, Chromotek) followed by α-mouse IgG Alexa Fluor 594 ...
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bioRxiv - Plant Biology 2022Quote: ... and then detected by the respective primary antibodies (Anti-Flag, Chromotek; Anti-HA, Abmart).
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bioRxiv - Cell Biology 2023Quote: ... Antibodies used for PLA include rabbit anti-GFP (1:200; ChromoTek # PABG1-20, RRID:AB_2749857)) and mouse α-Spectrin (1:50 ...
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bioRxiv - Physiology 2024Quote: ... Slides were then incubated with primary antibodies anti-RFP (rat, 1:100, ChromoTek [5F8]) and anti-Tubb3 (rabbit ...
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bioRxiv - Neuroscience 2020Quote: ... GFP fusion proteins in fixed cells were labelled with Alexa Fluor 647-coupled GFP nanobodies (GFP-booster gb2AF647-50, Chromotek, Germany) at 1:100 dilution ...
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bioRxiv - Microbiology 2022Quote: ... and 500 µg of protein from each sample was incubated with 35 µL pre-washed GFP-Trap magnetic beads (Chromotek, Inc.) for 1 h at 4 °C with rotation (1 mg/mL final lysate concentration) ...
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bioRxiv - Plant Biology 2021Quote: One milligram of total protein extract was mixed with 25 μl of GFP Nanobody/VHH coupled to agarose beads (GFP-Trap® Agarose, Chromotek) and incubated for 3 hours and 30 minutes at 4°C with end to end rocking ...
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bioRxiv - Cell Biology 2022Quote: ... Then 800-1000 µg of total protein lysates were added to 25 μl of anti-GFP magnetic beads (ChromoTek GFP-Trap) and tumbled end-over-end for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: For CoIP experiments 500 μl protein extract was mixed with 25 μl calibrated anti-GFP magnetic beads (ChromoTek GFP-Trap®) and incubated on a rotator for 2 h ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Plant Biology 2024Quote: ... between EIN2-C and HAF2 following study previous briefly: Total protein extracts from plants mentioned in the paper were immunoprecipitated by DYKDDDDK Fab-Trap™ agarose beads (ChromoTek) for two hours at 4 °C with gentle rotating and then beads were washed five times with ice-cold 1XPBS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and approximately 250 µg of protein was incubated with anti-GFP-conjugated magnetic agarose beads (ChromoTek GFP-Trap® Magnetic Agarose) for 1-1.5 hours ...
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bioRxiv - Plant Biology 2024Quote: ... Total proteins were extracted as described above and the extraction was mixed with 30 μL RFP-Trap® magnetic agarose beads (Chromotek) and rotated at 4 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were adjusted to 0.5% NP-40 and 500mg of protein incubated with pre-washed magnetic GFP-TRAP beads (ChromoTek gtma-20) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... IPs of GFP-tagged proteins were performed by incubating cleared cell lysates for 2 hours at 4°C with GFP-Trap® beads (ChromoTek) that had been pre-equilibrated with cold IPB ...
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bioRxiv - Cell Biology 2022Quote: ... the slides were incubated with relevant antibodies (anti-GFP: gb2AF488 from Chromotek (1:300 dilution), anti-ASY1 (1:500 dilution) ...
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bioRxiv - Neuroscience 2024Quote: ... Immunocytochemistry was performed with the following primary antibodies: rat anti-GFP (Chromotek, 50430-2-AP) 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Microbiology 2020Quote: ... Twenty milligram protein extracts were taken as input and the rest of the extracts were incubated with the GFP-Trap beads (ChromoTek, gta-20) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates (200 μg protein) from HEK-293T cells expressing WT-TRPC6-YFP was incubated with GFP M-trap beads (Chromotek GTD-20) to immunoprecipitate TRPC6 ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mL of protein extract was incubated with 60 μL (50% slurry) of GFP-trap magnetic agarose beads (ChromoTek, Planegg-Martinsried, Germany) for 3 h on a rotary shaker at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... One third of the whole cell lysate was pre-cleared with a 50:50 mixture of protein A/G magnetic beads and GFP-Trap (Chromotek, gtd-20) was pre-blocked with 1mg/mL ultra-pure BSA (AM2616 ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves and pulldown assay were carried out according to102 with the following modifications: For immunoprecipitation of GFP-tagged proteins 50 µl of GFP-Trap magnetic beads (Chromotek, Planegg-Martinsried, Germany) were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™ ...
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bioRxiv - Microbiology 2021Quote: Western blotting was performed as previously described46 using a commercial polyclonal anti Neon-Green antibody (Chromotek).