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Citations for ChromoTek :
151 - 188 of 188 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... 2mg/ml was used per IP experiments performed with camelid antibodies GFP-Trap Magnetic Agarose (Cat# gtma-20, Chromotek); RFP-Trap Magnetic Agarose (Cat# rtma-20 ...
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bioRxiv - Neuroscience 2022Quote: ... Another set of sections was incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... mCherry and GFP or EYFP signals in the neurons were enhanced by immunostainings using the following antibodies: rat anti-RFP (1:1,000, Chromotek) revealed by Cy3-conjugated donkey anti-rat secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections ipsilateral to the injection side were incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at +4°C with the primary antibody (rabbit anti-GFP, 1:600, Chromotek) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... A 30% suspension of protein A sepharose beads in RIPA buffer were incubated with the mNG antibody (32F6 Chromotek) for 1 hr at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was probed with the indicated primary antibody (rabbit anti-GFP [1:1500, Abcam, #ab290], mouse anti-RFP [1:1000, Chromotek, #6G6] ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was transferred to a fresh tube and incubated with anti-GFP antibody coupled with magnetic agarose (GFP-Trap, Chromotek) overnight at 4 °C on a rocking platform ...
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bioRxiv - Molecular Biology 2021Quote: ... They were then incubated with antibody-bead conjugate (Anti-FLAG® M2 Magnetic Beads, Millipore & Sigma or GFP-Trap_A, Chromotek) overnight at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The blot was incubated overnight on a shaker (4°C) with the primary antibody (anti-GFP 3H9, anti-RFP 6G6 (Chromotek) or anti-tRFP (Evrogen)) ...
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bioRxiv - Genetics 2021Quote: ... Pulldowns of the GFP-fusion proteins were performed by incubating tissue homogenate (7-day-old seedlings) or cell lysate (CHO cell lysate) with agarose beads that conjugated with GFP antibodies (GFP Nano-Trap, Chromotek). After incubations of 4-24 hours with gentle inverting ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... or to affinity purify ribosomes with translated RNA using GFP-TRAP beads (magnetic agarose beads coated with Alpaca anti-GFP VHH antibody, Chromotek). Incubation with the GFP-TRAP beads proceeded for 3 h at 4°C with gentle end-over-end mixing in a tube rotator and was followed by three washes with high salt buffer (350 mM KCl ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on 8% SDS-PAGE gels and detected by western blot using monoclonal mouse anti-HA (MMS-101R; Covance) or monoclonal mouse anti-RFP antibodies (6G6; ChromoTek) prior to secondary antibody treatment with polyclonal goat anti-mouse conjugated to horseradish peroxidase (115–035-146 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... The insoluble material was removed by centrifugation and the lysates were subjected to immunoprecipitation either by subsequent incubations with anti-Ecad antibody SHE78-7 (4 μg/ml) and protein A-beads or by incubation with GFP-trap beads (Chromotek). After incubation ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with primary antibody solution containing rabbit a-HA (1:500) (Cell Signalling) and rat a-RFP (1:500) (Chromotek) diluted in 3% BSA in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... portions of the blot were reacted against anti- mNeonGreen (mouse monoclonal antibody 32F6 from Chromotek, Inc., at 1:200 dilution), anti- HA (rabbit monoclonal antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... The CUT and TAG experiment was done for two biological replicates using antibodies anti-GFP (Chromotek, PABG1-100, 1:50), anti-YFP (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... 1:150 and Alex Fluor 488 conjugated site-specific dual monoclonal alpaca anti rabbit nano secondaries (single domain antibodies, VHHs, Chromotek) 1:1000 in blocking solution was applied to the sections at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Slides were subsequently blocked overnight in PBS containing 3% BSA before labelling with anti-mNeonGreen mouse monoclonal antibody (32F6; 1:300; Chromotek, Germany) followed by Alexa Fluor 488 goat anti-mouse antibody (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: Blotted proteins were detected using anti-GFP (LifeTechnologies, Darmstadt, Germany) and anti-red FP (RFP) antibody (ChromoTek GmbH, Planegg-Martinsried, Germany) (both ...
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bioRxiv - Neuroscience 2024Quote: ... incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rat anti-RFP, 1:1000, 5f8, Chromotek; mouse anti-5mC ...
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap® Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating ...
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bioRxiv - Cell Biology 2024Quote: ... were blocked for 1 h with TBST buffer containing 5% skimmed milk and then incubated for 1 h with mouse α-RFP antibody (ChromoTek). Membranes were washed with TBST and incubated for 1 h in TBST buffer with IRDye800 conjugated α-mouse antibody (LI-COR Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... GFP-Trap Magnetic Agarose beads or control beads without antibodies were used according to the manufactured protocol (both Chromotek, Planegg-Martinsried, Germany). The loaded magnetic beads were heated for 10 min at 95 °C in SDS loading buffer for the dissociation of purified proteins ...
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bioRxiv - Plant Biology 2021Quote: ... For immunoprecipitations, α-rabbit Trueblot agarose beads (eBioscience) coupled with α-FLS2 antibodies (Chinchilla et al., 2007) or GFP-Trap agarose beads (ChromoTek) were used and incubated with the crude extract for 3-4 h at 4 °C ...
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Mutual repression between JNK/AP-1 and JAK/STAT stratifies cell behaviors during tissue regenerationbioRxiv - Developmental Biology 2022Quote: ... Membrane was incubated with primary antibodies in PBS-T (1% Triton-X in 1xPBS) - rat anti-GFP (Chromotek, 3H9-100, 1:1000) and mouse anti-α-tubulin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed and treated for immunofluorescence with α-SINV C antibody at 1:200 dilution and GFP-Booster_Atto488 (ChromoTek GmbH, #gba488-100) at 1:500 dilution following the procedure described in (Garcia-Moreno et al. ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Cell Biology 2024Quote: ... thawed 90-nm-thick cryosections were incubated for 30 min at RT with a rabbit anti-GFP antibody (Chromotek, PABG1, 1:200 dilution) followed by protein A conjugated to 10 nm gold particles for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... The fixed cells were incubated for 1 hour in blocking solution (3% BSA in PBS) followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse mAb α-mNeonGreen (Chromotek #32f6-100), 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then probed overnight at 4°C with TBST buffer containing 1% non-fat dry milk and primary antibodies: mouse monoclonal anti-RFP (Chromotek, clone 6G6, 1:3,000), rabbit polyclonal anti-GFP (SYSY ...
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bioRxiv - Plant Biology 2020Quote: ... and the protein extract was separated on reducing SDS-PAGE and probed with commercial anti-RFP or anti-GFP antibodies (6G6, Chromotek or ab290, Abcam, respectively) to detect mCherry tagged MSBP1 or GFP-tagged ATI1 ...