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Citations for ChromoTek :
101 - 150 of 491 citations for Rat Segment polarity protein dishevelled homolog DVL 1 DVL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Arabidopsis stable lines expressing native VAP27-1-RFP or Mito-mCherry were stained with anti-TraB1a mouse primary antibody at a dilution of 1:500 and anti-RFP rat primary antibody (Chromotek), followed by secondary antibody incubation with FITC conjugated against mouse ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Plant Biology 2022Quote: ... a monoclonal RFP antibody raised in Rat (Chromotek, 5F8), followed by IRDye® 800CW goat anti-Rat IgG secondary antibody (Licor ...
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bioRxiv - Plant Biology 2020Quote: ... or c-Myc rat monoclonal antibody (Chromotek, 9E1-100) at a dilution of 1:1000 followed by goat anti-rat IgG horseradish peroxidase (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and a red fluorescent protein tag (tdTomato; ChromoTek, 5f8-100, 1:500) expressed by the rabies virus ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation was performed using crosslinked protein lysate and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... mCherry-fusion proteins were isolated by immunoprecipitation using the RFP-Trap®_MA kit (Chromotek). Lysates from transfected cells were prepared as above and incubated with RFP-trap magnetic agarose beads for 30 min to capture mCherry or mCherry-Lpg2411 ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates from cells expressing GFP tagged proteins were immunoprecipitated using a GFP-Trap Kit (Chromotek) as per manufacturer’s instructions and immunoblotting performed as above ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Plant Biology 2020Quote: ... A rat monoclonal anti-RFP antibody (Chromotek, cat. no. 5F8) was used at 1:1000 dilution and combined with a 1:5000 donkey anti-rat secondary antibody (Agrisera ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with anti-GFP antibodies (Rat monoclonal, ChromoTek) diluted 1/1500 in TBS containing 0.1% Tween overnight at 4C ...
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bioRxiv - Plant Biology 2020Quote: ... anti-HA and anti-FLAG antibodies produced in rat (Chromotek, UK) were used as primary antibodies ...
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bioRxiv - Genetics 2022Quote: ... 1 mg of protein extracts was incubated with agarose GFP traps (Chromotek, #GTA-20) pre-equilibrated with Buffer D (20mM HEPES pH 7.9 ...
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bioRxiv - Developmental Biology 2019Quote: ... GFP tagged proteins was detected with GFP-booster coupled with Atto488 (1:500; Chromotek). DNA was stained by DAPI (0.2 µg/ml ...
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bioRxiv - Plant Biology 2021Quote: ... RFP detection was performed with a rat anti-RFP 5F8 antibody (Chromotek) and an HRP-conjugated anti-rat antibody ...
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bioRxiv - Cell Biology 2019Quote: ... The mCherry fluorescent signal was enhanced with rat mAb anti-RFP (Chromotek). As secondary antibodies ...
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bioRxiv - Pathology 2020Quote: ... The blot was further probed with anti-Myc rat monoclonal antibody (Chromotek, 9E1) in a 1:1,000 dilution ...
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bioRxiv - Biochemistry 2019Quote: ... Protein (1 mg/mL) was incubated with 25 μL of GFP-Trap magnetic micro beads (ChromoTek), or anti-FLAG M2 magnetic beads (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated for 1 h with 25 μl GFP trap (ChromoTek; Planegg-Martinsried, Germany) on a rotating wheel at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mg total proteins were incubated with 15 µl pre-equilibrated magnetic GFP-TRAP beads (Chromotek) for 2 h on a rotator at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg of total protein was incubated with 25 μl of GFP-Trap® Magnetic Beads (ChromoTek) in 1 ml of extraction buffer at 4 °C for 2 h in a rotating wheel ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Neuroscience 2024Quote: ... Immunocytochemistry was performed with the following primary antibodies: rat anti-GFP (Chromotek, 50430-2-AP) 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Microbiology 2019Quote: ... washed twice with ice-cold PBS and immunoprecipitation of GFP-tagged proteins was performed using GFP-Trap®_A kit (Chromotek, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Successful affinity purification was confirmed by western blotting using Rat monoclonal anti-GFP antibody (3H9, Chromotek) and AffiniPure Donkey Anti-Rat IgG-HRP (712-035-153 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mL of the protein supernatant was incubated with 10 μL of magnetic GFP-Trap beads (Chromotek, Cat. No. gtma) or magnetic Myc-Trap beads (Chromotek ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 2 in CTD domain rat 3E10 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 5 in CTD domain rat 3E8 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... The signal of mCherry and GFP was enhanced using a rat mAb anti-RFP (Chromotek, 5f8-100) and a rabbit pAb anti-GFP antibody (BIOZOL/MBL ...
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bioRxiv - Microbiology 2022Quote: GFP-tagged target proteins and interacting proteins were affinity purified using GFP-trap agarose beads (Chromotek). Proteins were processed ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined and an equal amount of protein was mixed with TurboGFP-Trap Magnetic Agarose (Chromotek) for 1 hour at RT with agitation ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were detected using α-HA (Chromotek) and α-GFP antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... GFP fusion proteins were purified as follows: 200mg of protein extract was incubated with GFP-Trap Magnetic Agarose (ChromoTek) at 4°C for 20 minutes with gentle agitation ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Plant Biology 2023Quote: GFP-tagged or RFP-tagged proteins were immunoprecipitated from total leaf protein extract by incubation with GFP-Trap or RFP-Trap magnetic agarose beads (ChromoTek). The Co-immunoprecipitation assay was carried out according to ChromoTek’s instructions whereby 300 μl of protein extract was diluted with 500 μl of ice-cold dilution buffer [10 mM Tris-HCl (pH 7.5),150 mM NaCl ...
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bioRxiv - Developmental Biology 2021Quote: ... Lungs were collected at 5 days after tamoxifen injection and vibratome sections (350 µm) were stained with rat anti-GFP (to visualize membrane targeted GFP in Rosa26-mTmG; ChromoTek) or rabbit anti-RFP (to visualize cytoplasmic RFP in Rosa26-Confetti ...
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bioRxiv - Neuroscience 2019Quote: ... and 100 ng/ml GFP-Trap Protein (ChromoTek). Tissue samples were homogenized three times at 250 rpm and ten times at 750 rpm on a variable-speed homogenizer (Glas-Col ...