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Citations for ChromoTek :
51 - 100 of 104 citations for Recombinant Mouse Angiotensinogen serpin peptidase inhibitor clade A member 8 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Protein extracts containing GFP-tagged MIK2 or site-directed mutants were incubated with 20 µL of GFP-Trap beads (Chromotek) for 2 h with gentle mixing at 4 °C to immuno-precipitate receptor complexes ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells endogenously expressing C-terminal mEGFP-tagged MCM2 were transfected with plasmid (pCellCycleChromobody-RFP) containing RFP-PCNA chromobody (Chromotek, ccr) encoding single chain antibody recognizing endogenous PCNA protein ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation of tagged PC1 was done using anti-GFP nanobodies coupled to magnetic agarose beads (GFP-trap, Chromotek cat. no. gtma-10) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... washed twice with ice-cold PBS and immunoprecipitation of GFP-tagged proteins was performed using GFP-Trap®_A kit (Chromotek, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves and pulldown assay were carried out according to102 with the following modifications: For immunoprecipitation of GFP-tagged proteins 50 µl of GFP-Trap magnetic beads (Chromotek, Planegg-Martinsried, Germany) were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™ ...
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bioRxiv - Plant Biology 2024Quote: ... Coimmunoprecipitation of GFP- or RFP-tagged proteins from total leaf protein extract by incubation with GFP- or RFP-Trap magnetic agarose beads (ChromoTek, Planegg-Martinsried Germany) was performed as previously reported (Azevedo et al. ...
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bioRxiv - Plant Biology 2024Quote: Co-immunoprecipitations of the GFP-tagged FtsZ2-1 line was performed in biological triplicates using GFP-Trap Magnetic Particles M-270 (Chromotek, Planegg-Martinsried, Germany). In brief ...
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bioRxiv - Cell Biology 2022Quote: ... 8 µL (35 μL for ChIP-seq) of GFP-Trap magnetic agarose beads (Chromotek) were equilibrated in PBB buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Cell Biology 2022Quote: ... RFP mouse monoclonal antibody (ChromoTek GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse anti-RFP (Chromotek, 6G6) (diluted ...
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bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
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bioRxiv - Biochemistry 2023Quote: ... The rest of the supernatant was mixed with 8 µL anti-GFP beads (Chromotek, gta-20). The mixture was incubated for 2 h at 4 °C while rotating ...
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bioRxiv - Cell Biology 2022Quote: ... and Roche protease inhibitor cocktail) and subjected to GFP trap (ChromoTek). Immunoblotting was performed using standard procedures ...
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bioRxiv - Cell Biology 2021Quote: ... and Roche protease inhibitor cocktail) and subjected to GFP trap (ChromoTek). Immunoblotting was performed using standard procedures ...
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bioRxiv - Molecular Biology 2022Quote: ... protease inhibitors) and incubated with 15 μl of GFP_Trap magnetic beads (Chromotek) for 1h at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2024Quote: ... and Roche protease inhibitor cocktail) and subjected to GFP trap (ChromoTek, Germany). Immunoblotting was performed using standard procedures ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-RFP (1:1000, 6G6, Chromotek), rabbit anti-EGFR (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-RFP (1:1000, Chromotek-6G6), and rabbit anti-GST (1:2000 ...
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bioRxiv - Genetics 2022Quote: ... mouse anti-mNeonGreen (1:50, Chromotek, 32F6), rabbit anti-insulin (1:100 ...
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bioRxiv - Plant Biology 2023Quote: ... monoclonal anti-RFP produced in mouse (Chromotek), polyclonal anti-tBFP produced in rabbit (Evrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Halo-tag (mouse, 28a8 ChromoTek, 1:200), Alexa Fluor 647-direct labeled CETN1 (rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... mNeonGreen monoclonal mouse antibody (Chromotek, #32f6-100), p300 monoclonal mouse antibody (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2024Quote: ... monoclonal anti- RFP produced in mouse (Chromotek), and monoclonal anti-HA produced in rat (Chromotek) ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining supernatants were mixed with GFP-Trap magnetic beads (8 µL of slurry per sample; Chromotek, gtma-20) prewashed with IP Buffer [500 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... added with protease inhibitors) before incubation with GFP-Trap beads (Chromotek, Munich, Germany) for one hour at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on 8% SDS-PAGE gels and detected by western blot using monoclonal mouse anti-HA (MMS-101R; Covance) or monoclonal mouse anti-RFP antibodies (6G6; ChromoTek) prior to secondary antibody treatment with polyclonal goat anti-mouse conjugated to horseradish peroxidase (115–035-146 ...
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bioRxiv - Molecular Biology 2022Quote: ... produced in mouse and monoclonal anti-GFP (Chromotek) produced in rat were used as primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... An anti-mNG (mouse monoclonal IgG2c, ChromoTek 32f6, RRID:AB_2827566) or anti-TY (from BB2 hybridoma ...
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bioRxiv - Cell Biology 2020Quote: ... 450 μl of lysate was added to 8 μl (slurry volume) of washed GFP-Trap agarose beads (GTA-20, Chromotek GmbH, Germany). The tubes were incubated on an end-over-end rotator at 4°C for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... labeled using primary antibody (mouse anti-mNEONGreen, 1:1000, ChromoTek) and secondary antibody (goat anti-mouse conjugated with Alexa Fluor 555 ...
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bioRxiv - Cell Biology 2024Quote: ... 1% NP-40) with protease and phosphatase inhibitors were incubated with GFP-Trap Agarose beads (Chromotek) in a rotation wheel for 6 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... or mouse anti-mNeon Green (mNG) nanobody (ChromoTek, Planegg-Martinsried, Germany) to detect mNG fused to Tda1 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then sequentially incubated in mouse anti-mNeonGreen (IgG2c; Chromotek), followed by rat anti-mouse IgG(H+L)-AlexaFluor488 (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protease inhibitors) and incubated on a rotating wheel with 10 µl of precleared GFP-Trap Agarose beads (Chromotek) for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 3.6 ml of NE were supplemented with protease inhibitor cocktail and incubated with GFP-Trap Agarose resin (Chromotek), pre-equilibrated with buffer M (20 mM HEPES pH 7.9 ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies utilized for immunoblotting- mouse anti-mNeongreen (1:1000, Chromotek-32F6), rabbit anti-mNeongreen (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were probed with monoclonal mouse anti-mNeonGreen (mNeGr) IgGs (#32F6, Chromotek) and monoclonal mouse anti-GAPDH IgGs (#ab125247 ...
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bioRxiv - Plant Biology 2023Quote: ... rabbit anti-GFP (1:1000) and mouse anti-RFP (1:2000) (ChromoTek) monoclonal primary antibodies were used in combination with anti-rabbit or anti-mouse IgG horseradish peroxidase conjugated secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... or ii) a monoclonal anti-mNeonGreen antibody (anti-mouse IgG2c, 1:100, ChromoTek). Stumpy BSF were identified at the molecular level with a rabbit polyclonal anti-PAD1 antibody (kindly provided by Keith Matthews ...
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bioRxiv - Microbiology 2023Quote: ... or ii) a monoclonal anti-mNeonGreen antibody (anti-mouse IgG2c, 1:100, ChromoTek). CARP3 was detected using a polyclonal CARP3 antiserum (1:150 ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 mM dithiobis [succinimidyl propionate] containing 1x Complete Protease Inhibitor Cocktail) and then incubated with GFP-TRAP agarose beads (ChromoTek) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were incubated with the following antibodies: mouse α-mNeonGreen (1/500; 32F6, Chromotek) followed by α-mouse IgG Alexa Fluor 594 ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibody (mouse anti-mNeonGreen [32F6], Chromotek, 1:250) diluted in PBT + 1x WBR ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a 1:1,000 dilution of mouse anti-GFP [B-2] (SantaCruz Biotechnology) or a rat anti-RFP [5F8] (Chromotek) followed by a 1:5,000 dilution of the horseradish peroxidase-conjugated secondary antibody against mouse IgG (7076 ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was probed with the indicated primary antibody (rabbit anti-GFP [1:1500, Abcam, #ab290], mouse anti-RFP [1:1000, Chromotek, #6G6] ...
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bioRxiv - Plant Biology 2020Quote: ... The roots were incubated overnight at 4°C with the secondary anti-mouse (1:500; Jackson immunoreactive, conjugated to Rhodamine Red) and eba488-10 Spot Label ATTO488 (1:1000, Chromotek) diluted in PBS supplemented with 2% (w/v ...