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Citations for ChromoTek :
51 - 100 of 318 citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
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bioRxiv - Biochemistry 2024Quote: Protein extracts containing GFP-tagged MIK2 or site-directed mutants were incubated with 20 µL of GFP-Trap beads (Chromotek) for 2 h with gentle mixing at 4 °C to immuno-precipitate receptor complexes ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells endogenously expressing C-terminal mEGFP-tagged MCM2 were transfected with plasmid (pCellCycleChromobody-RFP) containing RFP-PCNA chromobody (Chromotek, ccr) encoding single chain antibody recognizing endogenous PCNA protein ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation of tagged PC1 was done using anti-GFP nanobodies coupled to magnetic agarose beads (GFP-trap, Chromotek cat. no. gtma-10) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... washed twice with ice-cold PBS and immunoprecipitation of GFP-tagged proteins was performed using GFP-Trap®_A kit (Chromotek, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves and pulldown assay were carried out according to102 with the following modifications: For immunoprecipitation of GFP-tagged proteins 50 µl of GFP-Trap magnetic beads (Chromotek, Planegg-Martinsried, Germany) were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™ ...
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bioRxiv - Plant Biology 2024Quote: ... Coimmunoprecipitation of GFP- or RFP-tagged proteins from total leaf protein extract by incubation with GFP- or RFP-Trap magnetic agarose beads (ChromoTek, Planegg-Martinsried Germany) was performed as previously reported (Azevedo et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
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bioRxiv - Immunology 2023Quote: The coding sequence of the Dnmt1-chromobody targeting the human Dnmt1 protein [37] (Uniprot : P26358 · DNMT1_HUMAN) and lamin-chromobody (ChromoTek) targeting the lamin D0 from Drosophila monogaster (Uniprot ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Nano-secondary AF568 (Chromotek, #srbAF568-1-100, 1:1000) diluted in PBS 10% (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: ... Nano-secondary AF568 (Chromotek, #srbAF568-1-100, 1:1000) prepared in PBS with 10% (wt/vol ...
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bioRxiv - Developmental Biology 2021Quote: ... (1:1000, Chromotek) primary antibodies and IRDye® 680RD Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were incubated in primary antibody (mouse monoclonal anti-PDF C7, 1:500-1:2000, DHSB, donated by Justin Blau; rabbit polyclonal anti-GFP 1:1000-2000, ChromoTek) solution in PBT+5% NGS at least overnight at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... rabbit anti-GFP (1:1000) and mouse anti-RFP (1:2000) (ChromoTek) monoclonal primary antibodies were used in combination with anti-rabbit or anti-mouse IgG horseradish peroxidase conjugated secondary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... at 1:2500 and anti-GFP Nano-body was used at 1:300 (Chromotek). Cells were washed 3 times for 5 mins with MBST (MOPS buffered saline with 0.05% Tween 20) ...
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bioRxiv - Cell Biology 2019Quote: ... GFP (Chromotek, 3H9, 1:50), lamin B1 (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... GFP booster (Chromotek, 1:500) and Alexa 568-conjugated phalloidin (Invitrogen 1:500) ...
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bioRxiv - Molecular Biology 2023Quote: ... mNeonGreen (1:500; Chromotek, 32f6). Coverslips were mounted using anti-fade reagent Prolong Gold containing DAPI (Life Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... α-GFP (Chromotek, 3H9, 1:2000), α-YFP (Venus ...
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bioRxiv - Cell Biology 2020Quote: ... anti-RFP (Invitrogen, #R10367, 1:1000 for WB and Chromotek #5F8, 1:1000 for WB), anti-phospho myosin light chain (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2022Quote: ... and 1:500 anti-rabbit nanobody coupled to Alexa Fluor 647 (Chromotek srbAF647-1-100). Slides were washed three times for two minutes in PBS in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... rat anti-RFP (1:20, Heinrich Leonhardt, 5F8) and rat anti-RFP (1:2000, ChromoTek-5F8). Fluorescent dyes DAPI (0.25 ng/µl ...
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bioRxiv - Plant Biology 2024Quote: ... membranes were probed with primary antibodies anti-GFP (1:1000) or anti-RFP (1:2000) (Chromotek) for 1 h ...
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bioRxiv - Plant Biology 2022Quote: ... or anti-RFP (1:2000; Chromotek) antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... rat anti-RFP (Chromotek, 1:4000); mouse anti-BicD (Clones 1B11 and 4C2 ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-booster (Chromotek; 1:300 dilution) was used to amplify GFP fluorescence for all tagged transgenes ...
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bioRxiv - Biochemistry 2021Quote: ... GFP-Booster_Atto488 (IF 1:300, ChromoTek), mouse anti-GFP (WB ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-Booster (1:1000; ABIN509419; Chromotek). The secondary antibodies used were goat anti-mouse IgG Alexa Fluor 568 conjugate (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... rat anti-RFP (1:1000, Chromotek), mouse anti-LN (1:10 ...
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bioRxiv - Cell Biology 2022Quote: ... rat anti-RFP (1:1000, Chromotek), mouse anti-Arl13B (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... GFP (1:5’000 Chromotek #3H9-100), GUS (1:1’000 Sigma Aldrich #G5545) ...
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bioRxiv - Neuroscience 2022Quote: ... anti-RFP (1:1000, rat, Chromotek), and anti-Lhx2 (1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... rat anti-RFP (1:500; Chromotek), rabbit anti-DsRed (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-RFP (5f8, Chromotek, 1:500), and anti-Myc (sc-40 ...
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bioRxiv - Molecular Biology 2020Quote: ... PCNA (Chromotek, 16D10-100, 1:500). Secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... rat anti-RFP (1:500, Chromotek), chicken anti-GFP (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... rat anti-RFP (Chromotek, 1:200); mouse anti-β-Gal (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... anti-RFP (1:1000, rat, Chromotek), anti-KN (1:4000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-GFP (1:1000, Chromotek, PABG1). Membranes were incubated for 1 h with either an IRDye secondary antibody (for LI-COR Odyssey imaging ...
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bioRxiv - Biochemistry 2023Quote: ... anti-Myc (ChromoTek, 1:1000, AB_2631398), anti-Vinculin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... anti-GFP (ChromoTek, 1:1000, 3H9), anti-RFP (ChromoTek ...
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bioRxiv - Biochemistry 2023Quote: ... anti-RFP (ChromoTek, 1:1000, 6G6). The secondary antibodies were ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti- RFP (1:1000, ChromoTek), and rabbit anti-GFP (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... anti-RFP (Chromotek #6g6, 1:1,000) or anti-Actin (Sigma #A0480 ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-CAP-D2 serum (1:1000) or rabbit anti-GFP conjugated with Alexa488 (1:1000, Chromotek, PABG1). And then with secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-RFP (1:1000, 6G6, Chromotek), rabbit anti-EGFR (1:1000 ...