Labshake search
Citations for ChromoTek :
51 - 65 of 65 citations for Recombinant Human CEACAM1 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... the extract prepared from OSC lines expressing endogenously tagged proteins of interest was incubated with magnetic GFP-Trap agarose (Chromotek) for 3h at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: Protein extracts containing GFP-tagged MIK2 or site-directed mutants were incubated with 20 µL of GFP-Trap beads (Chromotek) for 2 h with gentle mixing at 4 °C to immuno-precipitate receptor complexes ...
-
bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
-
bioRxiv - Cell Biology 2024Quote: ... IPs of GFP-tagged proteins were performed by incubating cleared cell lysates for 2 hours at 4°C with GFP-Trap® beads (ChromoTek) that had been pre-equilibrated with cold IPB ...
-
bioRxiv - Neuroscience 2024Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
-
bioRxiv - Genetics 2022Quote: ... Immunoprecipitation of tagged PC1 was done using anti-GFP nanobodies coupled to magnetic agarose beads (GFP-trap, Chromotek cat. no. gtma-10) following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
-
bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
-
bioRxiv - Synthetic Biology 2024Quote: ... FITC MEF and micromolar concentration values were obtained by calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250).
-
bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves and pulldown assay were carried out according to102 with the following modifications: For immunoprecipitation of GFP-tagged proteins 50 µl of GFP-Trap magnetic beads (Chromotek, Planegg-Martinsried, Germany) were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™ ...
-
bioRxiv - Plant Biology 2024Quote: ... Coimmunoprecipitation of GFP- or RFP-tagged proteins from total leaf protein extract by incubation with GFP- or RFP-Trap magnetic agarose beads (ChromoTek, Planegg-Martinsried Germany) was performed as previously reported (Azevedo et al. ...
-
bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
-
bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
-
bioRxiv - Immunology 2023Quote: The coding sequence of the Dnmt1-chromobody targeting the human Dnmt1 protein [37] (Uniprot : P26358 · DNMT1_HUMAN) and lamin-chromobody (ChromoTek) targeting the lamin D0 from Drosophila monogaster (Uniprot ...
-
bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...