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Citations for ChromoTek :
51 - 100 of 223 citations for Recombinant Cyan Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... or GFP-trap magnetic agarose (Chromotek, to precipitate GFP-tagged Cx43) were rocked with 200 µL of clarified cell lysate for 1 hour at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP-tagged Tail was pulled down with GFP-Trap® resin (ChromoTek) in a small column ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant myc and myc-BDNF were from Chromotek and Cusabio ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclear actin filaments were visualized with a GFP tagged nuclear actin chromobody (Chromotek) and filaments were induced with 8 µM Ionphore A23187 (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... and Venus-tagged PAXIP1 was precipitated using GFP-trap beads (Chromotek; gta-20) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... GFP-tagged M6 was purified using 20 µl GFP-Trap magnetic agarose (Chromotek). For other proteins ...
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bioRxiv - Cell Biology 2023Quote: ... and transfected 48 h prior to imaging with GFP-tagged PARP1 chromobody (Chromotek). For sensitization ...
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bioRxiv - Microbiology 2019Quote: ... GFP-trap immunoprecipitation of GFP-tagged VPg was accomplished using GFP-trap beads (Chromotek) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: GFP-tagged TBK1 was mixed with 20 µl of GFP-Trap agarose beads (Chromotek) at a final concentration of 1 µM ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescent lamin nanobody (lamin chromobody) plasmid was purchased from ChromoTek and transferred into the NcoI-XbaI site of pGEMHE ...
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bioRxiv - Plant Biology 2021Quote: ... The GFP tagged PIN7 variants were immunoprecipitated with the GFP-Trap Dynabeads (Chromotek, Planegg, Germany) according to the manufacturer ‘s description ...
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bioRxiv - Developmental Biology 2019Quote: ... For immunoprecipitation of GFP-tagged Imp and Bru1 15 μl of GFP-Trap® (Chromotek) were used ...
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bioRxiv - Biophysics 2021Quote: Immunoprecipitation was carried out using the GFP-tagged strain with the GFP-Trap Magnetic Agarose (Chromotek). Cells in the desired conditions were centrifuged at 12,000g for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP-tagged variants of the Sov NTD were immobilized on magnetic agarose GFP-Trap beads (ChromoTek) by incubation in PBS with 0.1 % Triton X-100 for 3 hours at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescent nuclear actin nanobody (nuclear actin chromobody) plasmid was purchased from ChromoTek and transferred into the HindIII-EcoRI site of pGEMHE ...
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bioRxiv - Cell Biology 2019Quote: ... The mCherry fluorescent signal was enhanced with rat mAb anti-RFP (Chromotek). As secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... GFP-tagged PD-1 variants or BTLA variants were IP’ed from the lysate using GFP-Trap (Chromotek, #gta-20). Equal fractions of the IP samples were subjected to SDS-PAGE and blotted with indicated antibodies.
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bioRxiv - Cell Biology 2022Quote: ... MEFs were washed extensively and GFP-booster (tagged with Alexa488, ChromoTek, gb2AF488-50, 1:500 in 3%BSA/PBS) and anti-rabbit-Alexa568 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and the retained Flag-tagged FlaA/B was eluted from the agarose beads using 3xFLAG®-peptide (Chromotek, Proteintech). For the co-IP of GlfM from S ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells endogenously expressing C-terminal mEGFP-tagged MCM2 were transfected with plasmid (pCellCycleChromobody-RFP) containing RFP-PCNA chromobody (Chromotek, ccr) encoding single chain antibody recognizing endogenous PCNA protein ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation of tagged PC1 was done using anti-GFP nanobodies coupled to magnetic agarose beads (GFP-trap, Chromotek cat. no. gtma-10) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
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bioRxiv - Plant Biology 2024Quote: Co-immunoprecipitations of the GFP-tagged FtsZ2-1 line was performed in biological triplicates using GFP-Trap Magnetic Particles M-270 (Chromotek, Planegg-Martinsried, Germany). In brief ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
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bioRxiv - Cell Biology 2020Quote: ... To enhance signal from fluorescent reporters we used GFP-booster coupled to Atto488 (gba488) and RFP-booster coupled to Atto594 (rba594) from Chromotek. Secondary antibodies used were from Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined and an equal amount of protein was mixed with TurboGFP-Trap Magnetic Agarose (Chromotek) for 1 hour at RT with agitation ...
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bioRxiv - Microbiology 2019Quote: ... The nanoboosters - single-domain alpaca antibody fragments covalently coupled to fluorescent dyes — GFP-Booster Atto488 and RFP-Booster Atto594 (Chromotek, Martinsried, Germany) — were used to boost eYFP and mCherry fluorescence ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were detected using α-HA (Chromotek) and α-GFP antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... GFP fusion proteins were purified as follows: 200mg of protein extract was incubated with GFP-Trap Magnetic Agarose (ChromoTek) at 4°C for 20 minutes with gentle agitation ...
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bioRxiv - Plant Biology 2023Quote: GFP-tagged or RFP-tagged proteins were immunoprecipitated from total leaf protein extract by incubation with GFP-Trap or RFP-Trap magnetic agarose beads (ChromoTek). The Co-immunoprecipitation assay was carried out according to ChromoTek’s instructions whereby 300 μl of protein extract was diluted with 500 μl of ice-cold dilution buffer [10 mM Tris-HCl (pH 7.5),150 mM NaCl ...
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bioRxiv - Neuroscience 2019Quote: ... and 100 ng/ml GFP-Trap Protein (ChromoTek). Tissue samples were homogenized three times at 250 rpm and ten times at 750 rpm on a variable-speed homogenizer (Glas-Col ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were purified by GFP-trap (ChromoTek) immunoprecipitation for 1 hour at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Protein complexes were immunoprecipitated using GFP-Trap (Chromotek) and washed 3 times with 50mM ammonium bicarbonate after the precipitation to remove detergent ...
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bioRxiv - Systems Biology 2023Quote: ... The proteins were immunoprecipitated using GFP-Trap (Chromotek) according to manufacturer’s protocol (with slight modifications) ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were incubated with GFP-Trap (ChromoTek) beads for 1h at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Biophysics 2020Quote: ... they were incubated with unlabelled (GFP-binding protein, Chromotek) and Abberior 635P conjugated Nanobody (GFP-booster ...
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bioRxiv - Cell Biology 2021Quote: ... protein lysates were added to RFP-Trap beads (Chromotek) and incubated for one hour at 4°C on a rotating shaker ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using anti-GFP antibody (3H9, Chromotek) or anti-alpha tubulin (T5168 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were immunoprecipitated with GFP-Trap beads (Chromotek, Germany), and analyzed by western blot with anti-GFP (Abiocode ...
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bioRxiv - Neuroscience 2021Quote: ... The protein complex pulled down by GFP-Trap beads (Chromotek) was washed three times with RIPA buffer and eluted in 30 μl of 1x Laemmli sample buffers by heating at 75°C for 10 min.
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bioRxiv - Cell Biology 2022Quote: ... GFP-GABARAP proteins were immunoprecipitated using GFP-TRAP beads (ChromoTek), incubating for 2hrs at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... or the 32F6 IgG2c monoclonal antibody against mNeonGreen protein (Chromotek). After several 5 min washes in PBS ...
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bioRxiv - Genetics 2023Quote: Protein complex was purified using GFP-Trap agarose beads (Chromotek) and separated by western blot ...
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bioRxiv - Plant Biology 2023Quote: ... protein extracts were incubated with GFP-Trap Agarose (Chromotek, Germany) at 4°C for 1 h with gentle rotation ...
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by GFP-Trap Agarose (Chromotek, gta).
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by RFP-Trap Agarose (Chromotek, rta). pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S: ...
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bioRxiv - Microbiology 2022Quote: ... mCherry fusion proteins were captured with RFP-Trap (Chromotek RTA-20) for 3 h at 4° C ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP fusion proteins were immunoprecipitated using GFP-Trap_MA (ChromoTek, Hauppauge, NY), recovered using a magnetic stand ...