Labshake search
Citations for ChromoTek :
51 - 100 of 499 citations for Mouse Chemokine C X3 C Motif Receptor 1 CX3CR1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed in the same buffer with 0.5% IGEPAL for 3-4 hours at 4 °C with GFP-trap resin (Chromotek). Beads were washed 3 times with the same buffer and bound proteins were eluted by addition of SDS loading dye and heating to 90°C for 10 min ...
-
bioRxiv - Neuroscience 2022Quote: ... for 30 min at 4°C and then with GFP-Trap Magnetic Particles M-270 (gtd-20, ChromoTek GmbH) for 1 h at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on 20 μl GFP-Trap beads (ChromoTek) for 30 min at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was cleared at 14,000 rpm for 10 min and incubated for 10 min rotating at 4 °C together with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek). Beads were washed four times with GFP pull-down buffer at 2,500 g for 2 min at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... Supernatants were centrifuged at 21,000 g at 4 °C for 10 min and then incubated for 10 min at 4 °C with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek) rotating ...
-
bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by filtering through miracloth followed by centrifugation (6,000 g, 30 min, 4°C) and then incubated with GFP-Trap (Chromotek) for 4h ...
-
bioRxiv - Plant Biology 2023Quote: ... rabbit anti-GFP (1:1000) and mouse anti-RFP (1:2000) (ChromoTek) monoclonal primary antibodies were used in combination with anti-rabbit or anti-mouse IgG horseradish peroxidase conjugated secondary antibodies ...
-
bioRxiv - Biochemistry 2021Quote: ... samples were spun down 10 min at 14000 rpm and 4°C and supernatant was added to GFP-Trap agarose beads from Chromotek and incubated for 1.5 hours at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... beads were collected (centrifugation for 10 min at 21,100 g and 4 °C) and the supernatant was added to 25 µl of blocked GFP-Trap Agarose (gta, ChromoTek).
-
bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 40 μl of GFP-Trap MA magnetic Beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
-
bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
-
bioRxiv - Plant Biology 2020Quote: ... The blot was incubated overnight on a shaker (4°C) with the primary antibody (anti-GFP 3H9, anti-RFP 6G6 (Chromotek) or anti-tRFP (Evrogen)) ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was incubated for 1 h at 4 °C with GFP-Selector beads (NanoTag) (Fig. 4A, B and D) or with Myc-trap beads (Chromotek) (Fig ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 15 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 150 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
-
bioRxiv - Plant Biology 2022Quote: ... The extracts were cleared by 10 min centrifugation at 12,000 rpm and the supernatant was incubated on a rotor wheel at 4°C for 3 h with 25 µl of GFP-Trap magnetic agarose bead slurry (Chromotek), pre-washed with extraction buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell lysates were cleared by centrifugation for 30 min at 4°C and incubated with GFP-trap magnetic agarose beads (Chromotek) for 1 h at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Supernatant was cleared at 14000 rpm for 10 minutes and incubated for 30 minutes rotating at 4 °C together with 25 µl pre-equilibrated Spot-Trap beads (Chromotek). Beads were washed 4 times with GFP PD buffer at 2500 g for 2 minutes at 4 ° C ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 4°C and added to GFP-Trap Magnetic agarose beads (Chromotek, cat #gtma-10) for 1 hr at 4°C with rotation ...
-
bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged for 20 min (full speed, 4°C) and the supernatant was incubated with mEGFP-Trap Agarose beads (ChromoTek) for 1 hour according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell lysate was centrifuged at 17,000 g for 10 min at 4 °C and supernatant was applied to 20 μl of GFP-Trap beads (Chromotek) and shaken for 2 hr at 4 °C ...
-
bioRxiv - Microbiology 2019Quote: ... and 200 ng total membrane proteins were incubated for 1 h at 4°C with gentle shaking on a roller mix with either 25 µl GFP-Trap® agarose beads (Chromotek) in a final volume of 100 µl 1% (w/v ...
-
bioRxiv - Cell Biology 2019Quote: U2OS cells endogenously expressing C-terminal mEGFP-tagged MCM2 were transfected with plasmid (pCellCycleChromobody-RFP) containing RFP-PCNA chromobody (Chromotek, ccr) encoding single chain antibody recognizing endogenous PCNA protein ...
-
bioRxiv - Cell Biology 2022Quote: ... The rest of the supernatants were incubated for 15 min at 4°C with 10 μl of GFP-Trap® Agarose beads (gta-10; Chromotek) to remove free GFP and spun at 1200 xg for 1 min at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: Cytoplasmic and nuclear fractions were incubated for 30 minutes at 4°C under agitation with 120 µL of control agarose beads (ChromoTek, Germany). Pre-cleared lysates were collected by a centrifuging 2 min at 400g (4°C) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting protein extracts were centrifuged at maximum speed on a table-top centrifuge for 15 minutes at 4 °C and the supernatant (soluble fraction) incubated with 25 μl GFP-trap magnetic beads (ChromoTek, gtma) for four hours at 4 °C under constant rotation ...
-
bioRxiv - Neuroscience 2022Quote: ... rat monoclonal anti-red fluorescent protein (RFP) (Chromotek, 1:1000) and goat anti-mCherry (1:1000) ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 20000 × g for 10 min at 4°C and added to pre-equilibrated beads (Chromotek, GTA-100) and rotated for 2 h at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated after centrifugation (13000x g for 10 min at 4°C) and incubated with 25 μl GFP Trap (Chromotek, gtm-20) magnetic beads for 1 hour at 4°C with rotation ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
-
bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 200 µL of sepharose beads for 1 h at 4°C under constant rotation and then subjected to immunoprecipitation with 50 µL of GFP Trap agarose beads (Chromotek, gta-20) for 2 h at 4°C under constant rotation ...
-
bioRxiv - Neuroscience 2024Quote: ... labeled using primary antibody (mouse anti-mNEONGreen, 1:1000, ChromoTek) and secondary antibody (goat anti-mouse conjugated with Alexa Fluor 555 ...
-
bioRxiv - Neuroscience 2022Quote: ... red fluorescent protein (RFP; rat, Chromotek: 5F8, RRID: AB_2336064; 1:2000) FoxP2 (mouse ...
-
bioRxiv - Neuroscience 2022Quote: ... and a red fluorescent protein tag (tdTomato; ChromoTek, 5f8-100, 1:500) expressed by the rabies virus ...
-
bioRxiv - Cell Biology 2021Quote: Primary antibodies utilized for immunoblotting- mouse anti-mNeongreen (1:1000, Chromotek-32F6), rabbit anti-mNeongreen (1:1000 ...
-
bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
-
bioRxiv - Microbiology 2020Quote: ... The supernatant was then transferred in 900 μl aliquots to fresh Eppendorf tubes and incubated with gentle head-over-tail agitation for 2 h at 4°C with 30 μl of anti-RFP or control beads (Chromotek rtma-2 and bmab-20, respectively) that had been washed in 1x lysis buffer.
-
bioRxiv - Microbiology 2021Quote: ... or ii) a monoclonal anti-mNeonGreen antibody (anti-mouse IgG2c, 1:100, ChromoTek). Stumpy BSF were identified at the molecular level with a rabbit polyclonal anti-PAD1 antibody (kindly provided by Keith Matthews ...
-
bioRxiv - Microbiology 2023Quote: ... or ii) a monoclonal anti-mNeonGreen antibody (anti-mouse IgG2c, 1:100, ChromoTek). CARP3 was detected using a polyclonal CARP3 antiserum (1:150 ...
-
bioRxiv - Genetics 2022Quote: ... 1 mg of protein extracts was incubated with agarose GFP traps (Chromotek, #GTA-20) pre-equilibrated with Buffer D (20mM HEPES pH 7.9 ...
-
bioRxiv - Developmental Biology 2019Quote: ... GFP tagged proteins was detected with GFP-booster coupled with Atto488 (1:500; Chromotek). DNA was stained by DAPI (0.2 µg/ml ...
-
bioRxiv - Plant Biology 2019Quote: ... RFP-fused proteins were detected using a rat anti-RFP antibody (1:5,000; ChromoTek). Light harvesting complex protein (LhcB1 ...
-
bioRxiv - Cell Biology 2022Quote: ... Slides were incubated with the following antibodies: mouse α-mNeonGreen (1/500; 32F6, Chromotek) followed by α-mouse IgG Alexa Fluor 594 ...
-
bioRxiv - Plant Biology 2020Quote: ... the membrane was probed with the indicated primary antibody (rabbit anti-GFP [1:1500, Abcam, #ab290], mouse anti-RFP [1:1000, Chromotek, #6G6] ...
-
bioRxiv - Plant Biology 2020Quote: ... The roots were incubated overnight at 4°C with the secondary anti-mouse (1:500; Jackson immunoreactive, conjugated to Rhodamine Red) and eba488-10 Spot Label ATTO488 (1:1000, Chromotek) diluted in PBS supplemented with 2% (w/v ...
-
bioRxiv - Plant Biology 2019Quote: ... mCherry proteins were detected by a rat anti-RFP antibody (5F8-100, 1:2,000, Chromotek), and then with a goat anti-rat IgG HRP-conjugated secondary antibody (AP202P ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein (1 mg/mL) was incubated with 25 μL of GFP-Trap magnetic micro beads (ChromoTek), or anti-FLAG M2 magnetic beads (Sigma) ...
-
bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated for 1 h with 25 μl GFP trap (ChromoTek; Planegg-Martinsried, Germany) on a rotating wheel at 4°C ...
-
bioRxiv - Plant Biology 2023Quote: ... GFP tagged proteins were detected using an anti-GFP antibody (1:4000; mAb rat 3H9; Chromotek) and a secondary anti-rat antibody (1:15000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mg total proteins were incubated with 15 µl pre-equilibrated magnetic GFP-TRAP beads (Chromotek) for 2 h on a rotator at 4 °C ...