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Citations for ChromoTek :
51 - 100 of 361 citations for 7 Bromo 3 4 dihydro 1H benzo e 1 4 diazepine 2 5 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Lysates incubated on a nutator at 4°C for two hours with 20μl of GFP-Trap beads (ChromoTek). GFP-Trap complexes were then washed four times in IP buffer and re-suspended in 2× SDS-PAGE sample buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysate was cleared by centrifugation and incubated for 2h at 4°C with magnetic GFP-Trap agarose (Chromotek). The beads were washed 3 x 10 min with Buffer 3 and were either used for mass spectrometry analysis or the proteins were eluted in 1× SDS buffer with 5 min incubation at 95°C for western blotting ...
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bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 200 μl of GFP-Trap_MA magnetic beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... for 30 min at 4°C and then with GFP-Trap Magnetic Particles M-270 (gtd-20, ChromoTek GmbH) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were then incubated for 1.5 h at 4 ⁰C with 25 µL RFP-Trap agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was cleared at 14,000 rpm for 10 min and incubated for 10 min rotating at 4 °C together with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek). Beads were washed four times with GFP pull-down buffer at 2,500 g for 2 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were centrifuged at 21,000 g at 4 °C for 10 min and then incubated for 10 min at 4 °C with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek) rotating ...
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bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by filtering through miracloth followed by centrifugation (6,000 g, 30 min, 4°C) and then incubated with GFP-Trap (Chromotek) for 4h ...
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bioRxiv - Biochemistry 2021Quote: ... samples were spun down 10 min at 14000 rpm and 4°C and supernatant was added to GFP-Trap agarose beads from Chromotek and incubated for 1.5 hours at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were collected (centrifugation for 10 min at 21,100 g and 4 °C) and the supernatant was added to 25 µl of blocked GFP-Trap Agarose (gta, ChromoTek).
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bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 40 μl of GFP-Trap MA magnetic Beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
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bioRxiv - Plant Biology 2020Quote: ... 50μg of total proteins were incubated 3H at 4°C with 30μl of GFP-Trap Agarose beads (Chromotek; gta-20) under gentle agitation for GFP-tagged protein purification ...
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bioRxiv - Plant Biology 2020Quote: ... The blot was incubated overnight on a shaker (4°C) with the primary antibody (anti-GFP 3H9, anti-RFP 6G6 (Chromotek) or anti-tRFP (Evrogen)) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was incubated for 1 h at 4 °C with GFP-Selector beads (NanoTag) (Fig. 4A, B and D) or with Myc-trap beads (Chromotek) (Fig ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were cleared by centrifugation for 30 min at 4°C and incubated with GFP-trap magnetic agarose beads (Chromotek) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatant was cleared at 14000 rpm for 10 minutes and incubated for 30 minutes rotating at 4 °C together with 25 µl pre-equilibrated Spot-Trap beads (Chromotek). Beads were washed 4 times with GFP PD buffer at 2500 g for 2 minutes at 4 ° C ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 4°C and added to GFP-Trap Magnetic agarose beads (Chromotek, cat #gtma-10) for 1 hr at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged for 20 min (full speed, 4°C) and the supernatant was incubated with mEGFP-Trap Agarose beads (ChromoTek) for 1 hour according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysate was centrifuged at 17,000 g for 10 min at 4 °C and supernatant was applied to 20 μl of GFP-Trap beads (Chromotek) and shaken for 2 hr at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... and 200 ng total membrane proteins were incubated for 1 h at 4°C with gentle shaking on a roller mix with either 25 µl GFP-Trap® agarose beads (Chromotek) in a final volume of 100 µl 1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... The rest of the supernatants were incubated for 15 min at 4°C with 10 μl of GFP-Trap® Agarose beads (gta-10; Chromotek) to remove free GFP and spun at 1200 xg for 1 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Cytoplasmic and nuclear fractions were incubated for 30 minutes at 4°C under agitation with 120 µL of control agarose beads (ChromoTek, Germany). Pre-cleared lysates were collected by a centrifuging 2 min at 400g (4°C) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting protein extracts were centrifuged at maximum speed on a table-top centrifuge for 15 minutes at 4 °C and the supernatant (soluble fraction) incubated with 25 μl GFP-trap magnetic beads (ChromoTek, gtma) for four hours at 4 °C under constant rotation ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 20000 × g for 10 min at 4°C and added to pre-equilibrated beads (Chromotek, GTA-100) and rotated for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated after centrifugation (13000x g for 10 min at 4°C) and incubated with 25 μl GFP Trap (Chromotek, gtm-20) magnetic beads for 1 hour at 4°C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were clarified by centrifugation at 12,000 rpm at 4°C for 10 min and then mixed with anti-RFP antibody coupled agarose beads (RFP-Trap, ChromoTek, Martinsried, Germany) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 200 µL of sepharose beads for 1 h at 4°C under constant rotation and then subjected to immunoprecipitation with 50 µL of GFP Trap agarose beads (Chromotek, gta-20) for 2 h at 4°C under constant rotation ...
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bioRxiv - Neuroscience 2021Quote: ... glycerol 5% and incubated for 2 h with 30 μl of pre-washed GFP-Trap magnetic agarose beads (gtma-20, ChromoTek, Planegg-Martinsried) using gentle rotation at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: MDA-MB-231 cells with Tks5αGFP were cultured on HDFC prepared on #1.5 coverglass for 2 days before stained for F-actin (phalloidin-Alexa Fluor-647) and Tks5αGFP (GFP-nanobody, ChromoTek GFP Vhh, # gt-250) using manufacturer recommended procedures ...
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bioRxiv - Neuroscience 2020Quote: ... The cleared lysate was incubated with an equivalent of 7 μl GFP-trap slurry (Chromotek) for 6 h at 4°C on a rotor ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were washed extensively and GFP-booster (tagged with Alexa488, ChromoTek, gb2AF488-50, 1:500 in 3%BSA/PBS) and anti-rabbit-Alexa568 (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were blocked in 3% BSA in PBS-T for 30 min before incubation with GFP-Booster _Atto488 (1:300; Chromotek), or mouse anti-G3BP1 in 3% BSA in PBS-T for 1 h at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... For the localization of RNAPII we used antibodies recognizing RNAPII phosphorylated at serine 5 (Chromotek, diluted 1:200) and serine 2 (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... Slides were subsequently blocked overnight in PBS containing 3% BSA before labelling with anti-mNeonGreen mouse monoclonal antibody (32F6; 1:300; Chromotek, Germany) followed by Alexa Fluor 488 goat anti-mouse antibody (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a 1:1,000 dilution of mouse anti-GFP [B-2] (SantaCruz Biotechnology) or a rat anti-RFP [5F8] (Chromotek) followed by a 1:5,000 dilution of the horseradish peroxidase-conjugated secondary antibody against mouse IgG (7076 ...
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bioRxiv - Plant Biology 2022Quote: ... 5 μL washed Myc magnetic beads (Chromotek) were added to the protein extracts of each sample and samples were then incubated on a rolling wheel at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... transduced cells were incubated with 100 μL Hybridization Buffer containing 2 μL 12.5 μM CF568-labled gRNA FISH probes and 1:2000 AF647-labeled anti-GFP nanobody (Chromotek, gb2AF647-50) for 4 hours in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... the PepNb sequence was ligated into BglII- and BstEII-digested backbone plasmids previously described as lamin-chromobody (eGFP) 7 and PCNA-chromobody (TagRFP) 33 (kindly provided by ChromoTek GmbH, Planegg-Martinsried, Germany). To generate homologous DNA for AAVS1 PepCB integration ...
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bioRxiv - Microbiology 2021Quote: ... The resin was then washed 3 times in GFP-Trap_A Wash buffer (Chromotek) and GFP-proteins were eluted using SDS sample buffer at 95°C for 10 min and analyzed by immunoblotting.
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bioRxiv - Biochemistry 2023Quote: ... lysates were incubated with 5 µL Myc Trap Agarose (Chromotek) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... or for 5 h with GFP-Trap magnetic agarose (Chromotek) at 4°C under rotation ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant was incubated with 5 μl of myc-Trap beads (Chromotek) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Myc-Trap agarose beads A (Chromotek, yta-2 Myc Trap_A) were prepared by 3 rounds of washing in extraction buffer and centrifugation at 1000 x g for 5min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... SPD-5 was then bound to 500 μL MBP-trap beads (Chromotek) and the column was washed 3X with buffer 4 (25mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... SPD-5 was then bound to 500 μL MBP-trap beads (Chromotek) and the column was washed 3X with buffer 4 (25mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM Tris pH 7.5 and 5 µl of equilibrated GFP-Trap beads (Chromotek) and incubated overnight at 4°C with constant mixing ...