Labshake search
Citations for Invivogen :
51 - 100 of 139 citations for hsa mir 296 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2023Quote: ... and mTLR8 cells suspended in 200 μl HEK-Blue detection medium (InvivoGen) were mixed with Aa EV at 7.7 × 107–6.2× 108 particles in a 96-well cell culture plate ...
-
bioRxiv - Microbiology 2024Quote: ... or SuperScript™ III Platinum™ One-Step qRT-PCR Kit for TaqMan reactions (InvivoGen, San Diego, CA, USA). An RT reaction for 30 minutes at 50°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 50,000 mTLR7 or Null2k cells suspended in 180 µl HEK-Blue Detection (Invivogen) medium were added to the respective wells ...
-
bioRxiv - Microbiology 2022Quote: ... the medium was exchanged with reconstituted HEK-Blue detection medium (InvivoGen, Toulouse, France). At 6 hpi ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were tested for Mycoplasma contamination using the Plasmotest-Mycoplasma Detection Test (InvivoGen) with the last test date on 06/07/2022 ...
-
bioRxiv - Bioengineering 2024Quote: ... 20 µL of supernatant was added to 180 µL QuantiBlue detection buffer (InvivoGen) and incubated at 37 °C and 5% CO2 for 1 h ...
-
bioRxiv - Immunology 2024Quote: ... 2.5 x 103 reporter cells were resuspended in HEK-Blue detection medium (Invivogen, hb-det2) in 96-well plates and stimulated with the appropriate agonist (Pam3CSK4 ...
-
bioRxiv - Immunology 2021Quote: ... SEAP amounts were then measured in the cell culture supernatants using Quanti-Blue Detection Media (InvivoGen) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... 20μL of cell culture supernatant were added to 180 μL of Quanti-Blue detection media (Invivogen) and developed in the tissue culture incubator for 20 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... and resuspended at a density of 2.8 × 105 cells/mL in HEK-Blue Detection media (Invivogen). Neutralizing antibodies against TLR2 ...
-
bioRxiv - Microbiology 2020Quote: ... reporter cell supernatants were analyzed for secreted alkaline phosphatase activity using HEK-Blue Detection reagent (Invivogen). Cytokines were quantified by enzyme-linked immunosorbent assay following the manufacturer’s protocol (R&D Systems) ...
-
bioRxiv - Microbiology 2021Quote: ... and levels of SEAP in the supernatants were determined using the detection medium QUANTI-Blue (Invivogen) after 24 h as per the manufacturer’s instructions using recombinant mouse IFN-β (PBL Assay Science ...
-
bioRxiv - Immunology 2020Quote: ... by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen), followed by absorbance measurement at 600 nm ...
-
bioRxiv - Microbiology 2021Quote: ... by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen), followed by absorbance measurement at 600 nm ...
-
bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions (150,000 cells/mL) were produced in HEK-Blue detection medium (InvivoGen, hb-det2). Cells were aliquoted at 200 uL per well of a 96-well plate containing 0.2 μL of a 1000x stock of each test compound in DMSO ...
-
bioRxiv - Microbiology 2023Quote: ... pneumoniae and control reagents were resuspended or diluted in pre-warmed HEK-BlueTM Detection medium (Invivogen). 5 x 104 HEK-BlueTM hTLR2 cells were mixed with 5 x 105 CFU S ...
-
bioRxiv - Cancer Biology 2022Quote: ... All cell lines were regularly tested for mycoplasma using the MycoStrip™ detection system (InvivoGen, Toulouse, France); all cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Immunology 2019Quote: Detection of bioactive human IFN-I was performed using reporter cell line HEK-Blue IFN-α/β (InvivoGen) as previously described12 ...
-
bioRxiv - Immunology 2024Quote: ... Cell lines were regularly checked for Mycoplasma contamination using the cell- based colometric HEK-Blue detection assay (Invivogen) and passaged maximally 20 times ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then stimulated at the indicated doses and times using LPS (Invivogen), IFN-γ (Peprotech ...
-
bioRxiv - Immunology 2021Quote: ... Half-area tissue-culture-treated 96 well plates were filled with 10μl of each diluted sample followed by 90μl of the reporter cell lines suspended in HEK-Blue Detection Media (Invivogen). Assays were performed with cell densities of 1.4 x 105 cells/well (mTLR2 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were seeded in a 96 well dish at 1.4×105 cells/mL (HEK-hTLR4) and 2.8×105 cells/mL (HEK-null2) in HEK detection media (Cat# hb-det3 Invivogen) as per manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... detached with a cell scraper and resuspended at a density of 2.8 × 105 cells/mL in HEK-Blue Detection media (Invivogen). Neutralizing antibodies against TLR2 ...
-
bioRxiv - Immunology 2023Quote: ... ISGF3 reporter activity was determined from cell culture supernatants using QUANTI-Blue detection reagents according to the manufacturer’s instructions (Invivogen).
-
bioRxiv - Immunology 2023Quote: ... NfkB activity was measured in the same supernatants using 20uL of supernatant with180uL QUANTI-Blue SEAP detection reagent (InvivoGen). The samples were then incubated at 37 °C for 1 h and absorbance was measured at 640 nm ...
-
bioRxiv - Microbiology 2024Quote: ... Twenty microliters of supernatant collected from infected cells was added to 180 μl of the SEAP detection solution (InvivoGen), followed by incubation at 37°C for ∼6 h ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were incubated for 30 min at room temperature (RT) and 0.2 µg/ml of Fc-h-dectin-hFc (Invivogen) was added to the UV-irradiated conidia and incubated for 1 h at RT ...
-
bioRxiv - Immunology 2019Quote: ... Cells were then washed 2 times followed by treatment with PMA (Invivogen; tlrl-pma) and 50ug/ml ionomycin (Invivogen ...
-
bioRxiv - Microbiology 2020Quote: ... supernatants from iBMDM infections were incubated with the reporter cell line and levels of SEAP in the supernatants were determined using the detection medium QUANTI-Blue (Invivogen) after 24 h as per manufacturer’s instructions using recombinant mouse IFNβ (PBL assay science ...
-
bioRxiv - Pathology 2020Quote: ... The conditioned media from each treatment group was analyzed for the presence of SEAP using QUANTIBlue™ detection reagent (Invivogen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... the activation of NF-κB drives the expression of the alkaline phosphatase enzyme that induces a color shift from pink to blue of the chromogenic substrate in the HEK-Blue Detection Media (Invivogen). These cells were cultured in complete DMEM medium composed of DMEM GlutaMAX (Gibco ...
-
bioRxiv - Immunology 2020Quote: ... Transfected HEK-Blue-KD-TLR5 cells were then gently flushed in PBS and resuspended in HEK-Blue Detection Media (Invivogen) at 2.8 × 105 cells/mL ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then pelleted (150 RCF, 5 min) and SEAP enriched supernatant was then utilized for detection media (QUANTI-Blue, Invivogen) activation.
-
bioRxiv - Bioengineering 2023Quote: ... 50 μL cell supernatant was collected from each sample and added to 150 μL of QUANTI-Blue SEAP detection medium (InvivoGen) and incubated for 2 h at 37 °C ...
-
bioRxiv - Systems Biology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination using cells cultured in non-antibiotic medium (PlasmoTest Mycoplasma Detection Assay, InvivoGen).
-
bioRxiv - Immunology 2023Quote: ... the luciferase detection reagent QUANTI-Luc 4 Lucia/Gaussia (Cat. #.: rep-qlc4lg1) and the SEAP detection reagent QUANTI-Blue 4 (Cat. #.: rep-qbs) were purchased from InvivoGen. Cell staining antibodies for the flow cytometry analysis ...
-
bioRxiv - Bioengineering 2024Quote: ... Supernatant (20 μL) was then removed from each well and added to 180 μL of QUANTI-Blue detection buffer (InvivoGen). The detection solution was incubated at 37 °C for 0.25-6 h until the lowest standard had visibly turned blue ...
-
bioRxiv - Immunology 2022Quote: ... cells were at 80 % confluence and the supernatant was discarded and replaced with 23 μL of control solutions (HEPES and water) or stimulants diluted in HEPES and water and 77 μL of HEK-Blue™ Detection medium (InvivoGen) (cell culture medium for real-time detection of SEAP) ...
-
bioRxiv - Immunology 2020Quote: ... Cells were cultured for 16 hours after which supernatant was harvested and SEAP reporter protein secretion was determined using QUANTI-Blue™ detection medium (Invivogen) and measuring absorption at 650 nm on a 550 Microplate reader (Bio-Rad ...
-
bioRxiv - Synthetic Biology 2021Quote: SEAP was detected in the media and in the lysate with an alkaline phosphatase detection medium – powder QUANTI-Blue™ (InvivoGen) as per the products instructions ...
-
bioRxiv - Microbiology 2022Quote: ... 180 µl/well of cells at a concentration of 2.8 × 105 cells/ml in HEK-Blue detection media (Invivogen; catalog #hb-det2) were plated in 96 well tissue-culture plate ...
-
bioRxiv - Developmental Biology 2023Quote: ... Media was removed prior to the addition of heat-inactivated serum diluted 1:20 in HEK Blue Detection media (Invivogen, #hb-det3). Cells were incubated for 48 hours and then absorbance was measured at 630 nm with a plate reader (SpectraMax) ...
-
bioRxiv - Immunology 2019Quote: ... before being cultured for indicated time points in FCS-free medium containing 1 µg/ml LPS (Invivogen), 1 μg/ml synthetic (B ...
-
bioRxiv - Cell Biology 2023Quote: ... Lines are karyotyped and verified for Mycoplasma contamination using PCR (InVivoGen) every 6 months.
-
bioRxiv - Immunology 2023Quote: ... NF-κB activation was determined after 20 h of incubation according to the manufacturer’s instructions by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen, San Diego, CA, USA). The plates were incubated for 2 hours at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... NF-kB activity was measured by combining 20 μL of supernatant with180 μL QUANTI-Blue SEAP detection reagent (InvivoGen, cat no. rep-qbs) in 96-well assay plate ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were allowed to adhere for 24 hours prior to removal of growth medium from each well and subsequent addition of 160uL of prewarmed HEK-Blue Detection Medium (Invivogen, cat. code hb-det). 40uL of NANP sample was added to each well ...
-
bioRxiv - Immunology 2021Quote: ... were transduced 3 times with lentivirus containing supernatant and selected with 1 μg/ml Puromycin (Invivogen, San Diego, USA). Surviving cells were expanded and referred to as HEK293 luciferase reporter cells.
-
bioRxiv - Immunology 2021Quote: ... For rescue assays cells were treated at the time of LPS priming with 10 μM AC-YVAD-CMK (Invivogen), 1 mM GNE9605 (Cayman Chemicals) ...
-
bioRxiv - Neuroscience 2020Quote: ... Then PCR product was inserted into pFUSE-hIgG2-Fc2 (InvivoGen, Cat. pfuse-hfc2) vector between Nco1 and BglII sites ...