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Citations for Invivogen :
101 - 150 of 195 citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The 279bp human AldA enhancer was PCR amplified from the pDRIVE5-GFP-10 plasmid (Invivogen). The HCMV enhancer was PCR amplified from the pDrive5 GFP-1 Promoter Test (Invivogen) ...
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bioRxiv - Immunology 2021Quote: ... IgA+ cells were detected by addition of goat anti-human IgA F(ab’)2 (InVivoGen) followed by anti-goat IgG AlexaFluor 594 (Jackson Immunoresearch ...
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bioRxiv - Immunology 2020Quote: HEK293 cells expressing murine TLR2 or human TLR4 and CD14/MD2 were purchased from InvivoGen. Cells were cultured in the presence of blasticidin (Nacalai Tesque ...
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bioRxiv - Immunology 2020Quote: ... animals received naked plasmid DNA or isolated recombinant proteins (30 μg/animal of designated recombinant protein, coupled with/without alum adjuvant (40 μg/animal) Alhydrogel® (Invivogen; vac-alu-250).
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bioRxiv - Microbiology 2021Quote: ... Two days post-transfection the media was replaced with DMEM containing 0.5% fetal bovine serum and NOD1 or NOD2 agonist [1 μg/ml C12-iE-DAP (Invivogen)+ 10 ng/ml human interferon gamma (AbD serotec) and 5 μg/ml L-18-MDP (InvivoGen) + 10 ng/ml human interferon gamma ...
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bioRxiv - Immunology 2021Quote: ... WIL2-S cells were opsonized with dilutions of anti-human CD20 – murine IgG2a (hcd20-mab10, InvivoGen) for 30 min followed by incubation with the diluted secondary antibody for 30 minutes and washed 3 times with HBSS ...
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bioRxiv - Immunology 2020Quote: ... Treatment of live leptospires was performed with 100 μg/mL of the human cathelicidin LL37 (Invivogen) in PBS for 2 hours.
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bioRxiv - Cancer Biology 2022Quote: ... We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen, #ant-bl-1) for 14 days or with 1 mg ml-1 puromycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... human γδT cells were pre-treated with p-JNK inhibitor (SP600125: 20uM, Invivogen, San Diego, CA) for 30min followed by IL-12/18/21 stimulation for 4 hours(43) ...
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bioRxiv - Genetics 2020Quote: The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: Human IgA was purified from fecal supernatants with Peptide M/ Agarose (InvivoGen, Cat. No. gel-pdm-2) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were transfected with mscv2.2 vectors containing human or murine NLRC4 were using lipofectamine 2000 (Invivogen). Briefly ...
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bioRxiv - Genomics 2024Quote: ... cells were stimulated for 45 minutes with 30 ng/ml human recombinant IL-1α (rcyec-hil1b; Invivogen) or 30 ng/ml human recombinant TNF-α (rcyc-htnfa ...
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bioRxiv - Immunology 2020Quote: ... recipient mice were administered (i.p.) 100 μg EndoFit Ovalbumin protein (InvivoGen). After three days ...
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bioRxiv - Immunology 2020Quote: ... 5μg of PR8-HA protein with Addavax (1:1 ratio; InvivoGen) and 0.5% tattoo ink was injected into the left quadriceps and right gastrocnemius (50 μL per site) ...
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bioRxiv - Genomics 2019Quote: ... supplemented with 5% human platelet lysate (Cook Regentec, #G34936) and 50 µg/mL Primocin (InvivoGen, #ant-pm-1). hASCs were cultured in the same media composition until 70-80% confluency and detached using TrypLE Select (Life Technology ...
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bioRxiv - Cell Biology 2022Quote: A549 lung carcinoma cells or A549 cells expressing human ACE2 or ACE2 and TMPRSS2 were obtained from Invivogen (a549 ...
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bioRxiv - Immunology 2020Quote: ... and 100 µl CpG (Murine CpG, ODN 1826, Human CpG ODN 2006 for pig and rabbit, InvivoGen, USA) were both added at 1mg/ml in WFI ...
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bioRxiv - Immunology 2019Quote: Detection of bioactive human IFN-I was performed using reporter cell line HEK-Blue IFN-α/β (InvivoGen) as previously described12 ...
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bioRxiv - Immunology 2021Quote: Human cell line THP-1 monocytes – ASC speck reporter cells were purchased from Invivogen (#thp-ascgfp, Invivogen, France) and called THP-1-ASC-GFP ...
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bioRxiv - Immunology 2023Quote: ... A549 with TMPRSS2 and human ACE2 overexpression cell line that was used in immunostaining was purchased from InvivoGen company (Cat.#a549-hace2tpsa ...
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bioRxiv - Immunology 2020Quote: OT-1 splenocytes were activated with 20 μg/ml OVA protein (Invivogen) and 100 U/ml hIL-2 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... the mice were challenged intravenously with 75 μg of OVA protein (InvivoGen) mixed with 75 μg of agonistic anti-CD40 antibodies ...
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bioRxiv - Immunology 2022Quote: The following recombinant proteins were purchased and mixed with adjuvant (AddaVax, Invivogen) before injection into mice.
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bioRxiv - Immunology 2022Quote: ... 1 mg/mL of whole OVA protein (Invivogen, San Diego, CA, USA) or γ-irradiated and CFSE stained B16F10 melanoma cells were added to the culture ...
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bioRxiv - Immunology 2023Quote: ... IFNβ protein levels were measured by LumiKineTMXpress hIFN-β 2.0 (InvivoGen, Germany) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... HEK-Blue™-hTLR2 cell line (Co-transfected with human TLR2 and SEAP reported gene) was purchased from InvivoGen. These cells were grown in DMEM media supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and MHC-I blocking antibody (W6/32; 50 ug/ml) in RPMI supplemented with human serum and primocin (Invivogen). Cells were harvested and stained with anti-CD3 FITC antibody (UCHT1 ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Immunology 2023Quote: Cells were primed with 2 µg/mL (human primary Mo) or 10 µg/mL (BLaER1 Mo) of Pam3CSK4 (Invivogen) for 2 h before activation ...
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bioRxiv - Microbiology 2023Quote: ... human cardiac fibroblasts in 96-well plate were transfected with 62 ng of high molecular weight poly(I:C) (InvivoGen) using TransIT-mRNA Transfection Kit (Mirus Bio) ...
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bioRxiv - Biochemistry 2023Quote: HKB11 PD-1 cells were used as stable pool generated by transfecting HKB11 cells with pMAX vector41 encoding human PD-1 (UniProt Q15116) and selection with 0.8 mg/mL geneticin (Invivogen).
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bioRxiv - Immunology 2019Quote: ... 293/NOD2 cells were obtained by stable transfection of HEK293 cells with the pUNO-hNOD2 plasmid which expresses the human NOD2 gene (Invivogen). THP1 cells were obtained from ATCC.
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bioRxiv - Microbiology 2021Quote: ... the human monocyte cell line THP1-Blue expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen) were cultured in RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: Non-glycosylated monoclonal human IgG1 antibody against human EGFR (hegfr-mab12, lot: EG12-39-01) and isotype control -Human IgG1 (bgal-mab1, lot: BG1-41-01) were purchased from InvivoGen. Human EGFR (Research Grade Cetuximab Biosimilar ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Human Embryonic Kidney HEK293-Null2 cells (Cat #hkb-null2) and HEK293-hTLR4 cells (Cat #hkb-htlr4) were obtained from Invivogen and are isogenic reporter cell lines ...
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bioRxiv - Microbiology 2021Quote: Human embryonic kidney HEK293T/17-hACE2 cells with stable expression of human angiotensin-converting enzyme ACE2 (AXON Neuroscience SE) were prepared by stable transfection of pDUO2-hACE2-TMPRSS2a (InvivoGen) with transfection reagent Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells that stably express human ACE2 (ACE2-293T) were cultivated in the presence of 2 μg/ml puromycin (Invivogen). Caco2 (human epithelial colorectal adenocarcinoma ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells stably expressing human TMPRSS2 were generated by lentiviral transduction followed by selection with blasticidin (40 μg/mL; Invivogen). SARS-CoV-2 strains hCoV-19/USA-WA1/2020 (NR-52281) ...
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bioRxiv - Molecular Biology 2023Quote: ... was fused to the Fc region (CH2 and CH3 domains) and hinge region of the human IgG1 heavy chain in the pFUSE-Fc1 vector (InvivoGen). Jag1ECD-Fc protein was expressed in Expi293F cells grown in Expi293F expression medium at 37°C in an 8% CO2 incubator with constant shaking ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2023Quote: The human monocytic THP-1 cell line (ATCC TIB-202) and the THP-1 DualTM reporter cell (InvivoGen, Thpd-nfis) were culture in RPMI supplemented with 10% heat-inactivated fetal calf serum ...
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bioRxiv - Immunology 2024Quote: Assessment of TLR4 and TLR5 activity was implemented using human embryonic kidney (HEK)-Blue-mTLR4 and HEK-Blue-mTLR5 cells respectively (Invivogen) using a protocol as previously described (Allen et al. ...
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bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein translation was inhibited with 50 µg·mL-1 of puromycin (ant-pr-1, Invivogen).
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bioRxiv - Immunology 2020Quote: ... The lyophilized powder was combined with 300 µg of CpG (Murine CpG, ODN 1826, Porcine, Rabbit, Human CpG ODN 2006 for pig and rabbit, InvivoGen, USA) which has been condensed with Polyethyleneimine (PEI ...
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bioRxiv - Microbiology 2023Quote: The ADCC reporter assay was performed by using Jurkat-Lucia NFAT-CD16 cells (human FcγRIII, V158) (Invivogen, Cat# jktl-nfat-cd16) as effector cells according to the manufacturer’s instruction with modifications ...
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bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
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bioRxiv - Microbiology 2021Quote: ... Rhesus Adenovirus 52 (RhAd52) (109 viral particles) or protein vaccines (50µg +100µg Adju phos (InvivoGen)) ...
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bioRxiv - Immunology 2020Quote: ... Slices were cultured overnight in complete media supplemented with 10 µg/mL OVA protein (Invivogen) or PBS ...