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Citations for Invivogen :
51 - 100 of 1023 citations for Thiol Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Mycoplasma detection tests were performed with PlasmoTest (Invivogen, code: rep-pt1). Cells were transiently co-transfected with 1 μg total cDNA using polyethylenimine (PEI ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 12-well plate in 1 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr) or 200 μg/mL hygromycin (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... growth media were replaced with HEK-blue detection media (Invivogen hb-det2) for SEAP assessment ...
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bioRxiv - Pathology 2023Quote: ... and mTLR8 cells suspended in 200 μl HEK-Blue detection medium (InvivoGen) were mixed with Aa EV at 7.7 × 107–6.2× 108 particles in a 96-well cell culture plate ...
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bioRxiv - Cancer Biology 2022Quote: ... 50,000 mTLR7 or Null2k cells suspended in 180 µl HEK-Blue Detection (Invivogen) medium were added to the respective wells ...
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bioRxiv - Microbiology 2022Quote: ... the medium was exchanged with reconstituted HEK-Blue detection medium (InvivoGen, Toulouse, France). At 6 hpi ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were tested for Mycoplasma contamination using the Plasmotest-Mycoplasma Detection Test (InvivoGen) with the last test date on 06/07/2022 ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of supernatant was added to 180 µL QuantiBlue detection buffer (InvivoGen) and incubated at 37 °C and 5% CO2 for 1 h ...
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bioRxiv - Immunology 2021Quote: ... 8 x 104 U937 cells per well of a 96 wells plate were exposed to 100 ng.mL-1 of phorbol 12-myristate 13-acetate (PMA; InvivoGen) for 48 h and primed with LPS at 1 μg.mL-1 for 3 h ...
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bioRxiv - Immunology 2020Quote: ... were harvested and incubated in 96-well plate (1×104 cells per well) with 250 μg/mL EndoFit Ovalbumin protein (InvivoGen) or 10 μg/mL OVA323–339 (ISQAVHAAHAEINEAGR ...
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bioRxiv - Immunology 2019Quote: ... BMDMs were seeded at a density of 2 × 105 cells/well in 48-well plates and pre-stimulated with 500 ng ml-1 of LPS (tlrl-peklps; InvivoGen) for 3 h ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were seeded at 1 × 105 cells/well in 96-well U-bottom plates in media containing 500 ng/mL Pam3CSK4 (Invivogen) for 4-6 hours then treated with either Val-boroPro (10 μM ...
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bioRxiv - Immunology 2022Quote: WT and LRBA-KO HEK293T cells were plated on 12 mm cover slides in a 24-wells plate and then treated for 1 h with 50 µM Rapamicin (Invivogen), 333 nM Torin 1(Invivogen) ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were seeded as 0.3*106 cells in 6-wells plate followed by treatment for 4 h with 333 nM of Torin 1 (InvivoGen) alone or in combination with 200 nM of Bafilomycin A1 for 1 h.
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bioRxiv - Immunology 2024Quote: ... 2.5 x 103 reporter cells were resuspended in HEK-Blue detection medium (Invivogen, hb-det2) in 96-well plates and stimulated with the appropriate agonist (Pam3CSK4 ...
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bioRxiv - Immunology 2021Quote: ... SEAP amounts were then measured in the cell culture supernatants using Quanti-Blue Detection Media (InvivoGen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 20μL of cell culture supernatant were added to 180 μL of Quanti-Blue detection media (Invivogen) and developed in the tissue culture incubator for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... and resuspended at a density of 2.8 × 105 cells/mL in HEK-Blue Detection media (Invivogen). Neutralizing antibodies against TLR2 ...
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bioRxiv - Microbiology 2020Quote: ... reporter cell supernatants were analyzed for secreted alkaline phosphatase activity using HEK-Blue Detection reagent (Invivogen). Cytokines were quantified by enzyme-linked immunosorbent assay following the manufacturer’s protocol (R&D Systems) ...
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bioRxiv - Microbiology 2021Quote: ... and levels of SEAP in the supernatants were determined using the detection medium QUANTI-Blue (Invivogen) after 24 h as per the manufacturer’s instructions using recombinant mouse IFN-β (PBL Assay Science ...
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bioRxiv - Immunology 2020Quote: ... by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen), followed by absorbance measurement at 600 nm ...
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bioRxiv - Microbiology 2021Quote: ... by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen), followed by absorbance measurement at 600 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions (150,000 cells/mL) were produced in HEK-Blue detection medium (InvivoGen, hb-det2). Cells were aliquoted at 200 uL per well of a 96-well plate containing 0.2 μL of a 1000x stock of each test compound in DMSO ...
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bioRxiv - Microbiology 2023Quote: ... pneumoniae and control reagents were resuspended or diluted in pre-warmed HEK-BlueTM Detection medium (Invivogen). 5 x 104 HEK-BlueTM hTLR2 cells were mixed with 5 x 105 CFU S ...
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bioRxiv - Bioengineering 2023Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Biochemistry 2023Quote: ... All stock plates were treated with plasmocin (InvivoGen) as per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were regularly tested for mycoplasma using the MycoStrip™ detection system (InvivoGen, Toulouse, France); all cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then sorted for GFP-positive to enrich for CROP-seq-vector-positive cells and plated on matrigel-coated plates in mTesr1supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and Y-27632 (final concentration 5µM) ...
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bioRxiv - Developmental Biology 2022Quote: ... dTomato-positive cells were sorted on a BD FACS Aria III using BD FACSDiva 8.0.1 Software using a 100 µm nozzle and cultured on Martigel-coated plates in mTeSR Plus supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and 25 µm Y-27632 ...
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bioRxiv - Molecular Biology 2019Quote: ... plates included 200 µg/mL Hygromycin B Gold (InvivoGen) or 133 µg/mL Nourseothricin (Gold Biotechnology) ...
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bioRxiv - Immunology 2019Quote: Detection of bioactive human IFN-I was performed using reporter cell line HEK-Blue IFN-α/β (InvivoGen) as previously described12 ...
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bioRxiv - Immunology 2024Quote: ... Cell lines were regularly checked for Mycoplasma contamination using the cell- based colometric HEK-Blue detection assay (Invivogen) and passaged maximally 20 times ...
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bioRxiv - Immunology 2021Quote: ... NLRP3-/- iBMDMs or NLRP3-/- iBMDMs reconstituted with mScarlet-tagged NLRP3 were seeded at 0.5×106 cells/well on a 12-well tissue culture plate and on the next day treated or not with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h or with 1 μg/ml LPS for 2.5 h and additionally with 20 μM MCC950 (Millipore Sigma ...
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bioRxiv - Immunology 2021Quote: ... Half-area tissue-culture-treated 96 well plates were filled with 10μl of each diluted sample followed by 90μl of the reporter cell lines suspended in HEK-Blue Detection Media (Invivogen). Assays were performed with cell densities of 1.4 x 105 cells/well (mTLR2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were seeded in a 96 well dish at 1.4×105 cells/mL (HEK-hTLR4) and 2.8×105 cells/mL (HEK-null2) in HEK detection media (Cat# hb-det3 Invivogen) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... detached with a cell scraper and resuspended at a density of 2.8 × 105 cells/mL in HEK-Blue Detection media (Invivogen). Neutralizing antibodies against TLR2 ...
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bioRxiv - Immunology 2023Quote: ... ISGF3 reporter activity was determined from cell culture supernatants using QUANTI-Blue detection reagents according to the manufacturer’s instructions (Invivogen).
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bioRxiv - Immunology 2023Quote: ... NfkB activity was measured in the same supernatants using 20uL of supernatant with180uL QUANTI-Blue SEAP detection reagent (InvivoGen). The samples were then incubated at 37 °C for 1 h and absorbance was measured at 640 nm ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microliters of supernatant collected from infected cells was added to 180 μl of the SEAP detection solution (InvivoGen), followed by incubation at 37°C for ∼6 h ...
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bioRxiv - Microbiology 2020Quote: ... supernatants from iBMDM infections were incubated with the reporter cell line and levels of SEAP in the supernatants were determined using the detection medium QUANTI-Blue (Invivogen) after 24 h as per manufacturer’s instructions using recombinant mouse IFNβ (PBL assay science ...
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bioRxiv - Pathology 2020Quote: ... The conditioned media from each treatment group was analyzed for the presence of SEAP using QUANTIBlue™ detection reagent (Invivogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the activation of NF-κB drives the expression of the alkaline phosphatase enzyme that induces a color shift from pink to blue of the chromogenic substrate in the HEK-Blue Detection Media (Invivogen). These cells were cultured in complete DMEM medium composed of DMEM GlutaMAX (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Transfected HEK-Blue-KD-TLR5 cells were then gently flushed in PBS and resuspended in HEK-Blue Detection Media (Invivogen) at 2.8 × 105 cells/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then pelleted (150 RCF, 5 min) and SEAP enriched supernatant was then utilized for detection media (QUANTI-Blue, Invivogen) activation.
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bioRxiv - Bioengineering 2023Quote: ... 50 μL cell supernatant was collected from each sample and added to 150 μL of QUANTI-Blue SEAP detection medium (InvivoGen) and incubated for 2 h at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination using cells cultured in non-antibiotic medium (PlasmoTest Mycoplasma Detection Assay, InvivoGen).
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bioRxiv - Immunology 2023Quote: ... the luciferase detection reagent QUANTI-Luc 4 Lucia/Gaussia (Cat. #.: rep-qlc4lg1) and the SEAP detection reagent QUANTI-Blue 4 (Cat. #.: rep-qbs) were purchased from InvivoGen. Cell staining antibodies for the flow cytometry analysis ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatant (20 μL) was then removed from each well and added to 180 μL of QUANTI-Blue detection buffer (InvivoGen). The detection solution was incubated at 37 °C for 0.25-6 h until the lowest standard had visibly turned blue ...
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bioRxiv - Immunology 2022Quote: ... cells were at 80 % confluence and the supernatant was discarded and replaced with 23 μL of control solutions (HEPES and water) or stimulants diluted in HEPES and water and 77 μL of HEK-Blue™ Detection medium (InvivoGen) (cell culture medium for real-time detection of SEAP) ...