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Citations for Invivogen :
51 - 100 of 442 citations for Siglec 2 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... human p27 (Invivogen; 500 ng), and SV40 large T antigen (20 ng ...
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bioRxiv - Immunology 2023Quote: ... the human cDNA sequence (Invivogen) was subcloned into the pcDNA3 vector between the HindIII and EcoRV restriction sites followed by transfection of 5 µg plasmid into BAFF/CD40L double positive fibroblasts using Lipofectin reagent (Thermo) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed twice with PBS and stained with Fc-hDectin-1a (Invivogen) and Calcofluor white (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... we used engineered HEK293 cell lines (HEK-Blue) that stably express either mouse TLR9 (mTLR9) and an NF-κB reporter gene (Invivogen, San Diego, CA). In brief ...
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bioRxiv - Cancer Biology 2020Quote: ... M25 scFv was subcloned into either the Fc fusion expression vector pFUSE-hIgG1 Fc2 (InvivoGen) or a modified Abvec expression vector that was originally provided by Dr ...
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bioRxiv - Immunology 2019Quote: ... into expression plasmids adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: cDNA encoding a truncated human ACE2 fused to human albumin was sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vector was transiently transfected into Expi293F cells in suspension (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... A549 lung carcinoma cells expressing human ACE2 and human TMPRSS2 (A549.ACE2+.TMPRSS2+; cat n° a549-hace2tpsa, Invivogen) were grown in DMEM/10% FBS supplemented with 100 µg/ml Normocin ...
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bioRxiv - Immunology 2021Quote: ... In some assays human anti-RBD (Invivogen) of known antibody concentration was used as standard ...
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bioRxiv - Immunology 2022Quote: ... 2 ug/mL Pam3CSK4 (TLR1/2 agonist, Invivogen), and 1 ug/mL FSL-1 (TLR2/6 agonist ...
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bioRxiv - Bioengineering 2020Quote: Nanobodies targeting RBD were expressed and secreted as Fc fusions by cloning into pFUSE-hlgG1-Fc2 (Invivogen) using the NcoI and EcoRI restriction sites or by Gibson assembly ...
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bioRxiv - Biophysics 2019Quote: The coding sequences of antibody Fc heterodimerization domains were amplified from the pFUSE-hIgG1-Fc1 plasmid (Invivogen) containing two charged mutations on each opposing domain57,58 ...
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bioRxiv - Immunology 2019Quote: ... before being cultured for indicated time points in FCS-free medium containing 1 µg/ml LPS (Invivogen), 1 μg/ml synthetic (B ...
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bioRxiv - Immunology 2021Quote: Anti-human CD20 murine IgG2a (hcd20-mab10, InvivoGen) and mouse anti-Biotin mIgG2a [3E6] (ab36406 ...
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bioRxiv - Cell Biology 2021Quote: ... Human TMPRS2 (pUNO1-hTMPRSS2a) was ordered from InvivoGen. Full length SARS-CoV-2 Spike plasmid (VG40589-UT ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Recombinant human TNF-α was purchased from InvivoGen. The antiretroviral drugs efavirenz ...
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bioRxiv - Bioengineering 2021Quote: ... and 2 µl/ml primicin (Ant-pm-2, InvivoGen) at 37°C for 19 hours.
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bioRxiv - Bioengineering 2022Quote: ... and 2 μl/ml primocin (Ant-pm-2, InvivoGen) at 37°C for 19 hours ...
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bioRxiv - Bioengineering 2020Quote: ... Single chain human IL-12 was purchased from Invivogen. Blinatumomab (anti-hCD19-CD3 ...
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bioRxiv - Microbiology 2024Quote: ... human carcinoma epithelial lung A549 (Invivogen Inc, Toulouse, France), and Monkey kidney normal Vero E6 (CCL-81 ...
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bioRxiv - Microbiology 2024Quote: ... human carcinoma epithelial lung A549 (Invivogen Inc, Toulouse, France), human microglial clone 3 HCM3 (ATCC ...
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bioRxiv - Microbiology 2023Quote: Human HepG2 liver carcinoma AhR-Lucia reporter cells (InvivoGen) were cultured according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Endogenously tagged cell lines were maintained in HMI-9 supplemented with 20 % (v/v) FCS at 37 °C in 5% C02 and maintained in 10 µg.mL-1 Blasticidin (InvivoGen). Parasite density was monitored using a haemocytometer.
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bioRxiv - Microbiology 2022Quote: ... PCR products were gel purified and cloned into heavy and kappa expression plasmids52,53 adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 ampicillin resistant vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the mouse hinge and IgG2a heavy chain Fc region (pFUSE-mIgG2a-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide and was subcloned into a pMSGV retroviral vector followed by enhanced (e)GFP reporter gene cassette using PCR and standard molecular cloning techniques to generate pMSGV-A4-Fc-T2A-eGFP ...
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bioRxiv - Immunology 2023Quote: ... and 10% FCS in a 96 well ‘U’ bottomed plate and stimulated with 1μg/mL LPS-EB Ultrapure (Invivogen) and Brefeldin A (eBioScience) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were incubated for 30 min at room temperature (RT) and 0.2 µg/ml of Fc-h-dectin-hFc (Invivogen) was added to the UV-irradiated conidia and incubated for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: Biological activity of laminarin in LamOVA conjugates was analyzed by ELISA using a soluble murine Fc-Dectin-1a receptor (InvivoGen). Hypoallergenicity was analyzed in vitro as a measure of rat basophil leukemia cell (RBL-2H3 ...
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bioRxiv - Biochemistry 2020Quote: ... 17 ECD-10His (extracellular domain) was generated by Gibson cloning and adapted from the commercially available pFUSE-hIgG1-Fc (InvivoGen) vector ...
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bioRxiv - Immunology 2019Quote: ... KX571250-1324) were cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... three BALB/c mice were each injected intraperitoneally (i.p.) with 100 μg of RBD-Fc fusion protein formulated with 500 μg of aluminum hydroxide (Alhydrogel, Invivogen, USA) and 25 μg of CpG (Sangon ...
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bioRxiv - Immunology 2020Quote: Total spleen cells were cultured at a density of 1 × 106 cells/mL in RPMI 1640 supplemented with 10% fetal calf serum (FCS) with 5 ng/mL LPS (Invivogen). For 4-hydroxytamoxifen (OHTAM ...
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bioRxiv - Immunology 2023Quote: ... These Gibson sequences are necessary for cloning of the VH and VL into our rabbit IgG1 expression vectors which are based on the pFUSE-rIgG-Fc (InvivoGen) vectors.75,76 For this second PCR ...
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bioRxiv - Immunology 2021Quote: ... reagents in human TLR1-9 Agonist kit (InvivoGen, tlrl-kit1hw) were diluted to working concentration in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... 2’,3’-cGAMP (Invivogen) at 10 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... or on solid YPD-agar (1% yeast extract, 2% peptone, 2% D-glucose, 2% agar) and selected with 100 µg/ml Zeocin® (InvivoGen). For small scale expression screening ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by selection using 2 μg/ml of puromycin (Invivogen, 58-58-2) for 2–3 days starting from 2 days after infection ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added in order to generate a standard curve for each assay ...
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bioRxiv - Immunology 2020Quote: ... Human THP-1 monocyte-like cells (THP-1 Lucia ISG, Invivogen) were maintained in RPMI 1640(Thermo Fisher cat ...
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bioRxiv - Microbiology 2022Quote: A549-hACE2 lung carcinoma cells expressing the human ACE2 protein (Invivogen) were maintained in DMEM with 4.5 g/L glucose and 2 mM L-glutamine (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added to generate a standard curve for each assay ...
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bioRxiv - Immunology 2023Quote: ... IgG3 concentrations were measured by an IgG3 Human ELISA Kit (Invivogen).
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bioRxiv - Immunology 2023Quote: Human monocytes were stimulated with 10ug/mL TL8 (Invivogen, tlrl-tl8506), 10ug/mL ssPolyU(Invivogen ...
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bioRxiv - Genomics 2024Quote: ... or 30 ng/ml human recombinant TNF-α (rcyc-htnfa; Invivogen), respectively ...
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bioRxiv - Immunology 2022Quote: ... 2′3′- cyclic-di-GMP-AMP (2′3′-cGAMP) (Invivogen cat. no. tlrl-nacga23), and human IFN-β (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... or Alum (Alhydrogel 2%, InvivoGen) at 50% v/v at final volume of 30µl per dose ...
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bioRxiv - Immunology 2020Quote: ... R848 (2 μg/mL, InvivoGen), CHIKV-LR at a multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 2% Primocin (Invivogen) at 4°C and transported on ice to be processed within 24 hours for organoid culture ...