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Citations for Invivogen :
1 - 50 of 82 citations for Recombinant Mouse Cd14 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Genomics 2020Quote: HEK293/hTLR4A-MD2-CD14 Cells (Invivogen) were selected for functional follow up of the IRF1 trans-eQTL because of the ease of transfection of HEK293 cells and the addition of the TLR4 receptor ...
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bioRxiv - Immunology 2020Quote: THP1-XBlue-CD14 reporter cells (InvivoGen, San Diego, USA) were seeded in 96 well plates in phenol red RPMI ...
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bioRxiv - Microbiology 2021Quote: THP1-XBlue-CD14 reporter cells (InvivoGen, San Diego, USA) were seeded in 96 well plates (180,000 cells/well ...
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bioRxiv - Immunology 2022Quote: HEK-293 cells stably expressing TLR4/MD2/CD14 were purchased from Invivogen. Cells were seeded at 3.5 × 104 live cell/well in 96 well plates overnight and were transfected with NF-κB-luciferase reporter plasmid (50 ng/well ...
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bioRxiv - Immunology 2021Quote: ... CD14+ cells were cultured o/n with 100 ng/mL LPS (Invivogen) and treated with 300 nM JQ1(- ...
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bioRxiv - Systems Biology 2020Quote: ... HEK 293 stable TLR4-MD2-CD14 cell line was also purchased from InvivoGen. All chemicals were HPLC-grade unless specifically indicated ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen) and 10% FCS ...
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bioRxiv - Immunology 2020Quote: HEK293 cells expressing murine TLR2 or human TLR4 and CD14/MD2 were purchased from InvivoGen. Cells were cultured in the presence of blasticidin (Nacalai Tesque ...
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bioRxiv - Immunology 2023Quote: ... These analyses were conducted using either a HEK293T TLR4-CD14-MD2 IL-18 reporter line (Invivogen) or the RAW-Blue Cell assay (Invivogen) ...
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bioRxiv - Microbiology 2020Quote: ... Human CD14+ monocytes (2×106/ml) were pre-incubated with human anti-Dectin-1 (10μg/ml, Invivogen), anti-TLR2 blocking antibodies (10μg/ml ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Recombinant human TNF-α was purchased from InvivoGen. The antiretroviral drugs efavirenz ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... animals received naked plasmid DNA or isolated recombinant proteins (30 μg/animal of designated recombinant protein, coupled with/without alum adjuvant (40 μg/animal) Alhydrogel® (Invivogen; vac-alu-250).
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bioRxiv - Microbiology 2020Quote: ... prepared from recombinant flagellin from Salmonella typhymurium (RecFLA-ST, Invivogen) in sterile water was also added to the plate in triplicate ...
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bioRxiv - Immunology 2022Quote: ... 40µg recombinant RBD plus 5µg LPS-EB VacciGrade™ (InvivoGen) was given to isoflurane anesthetized mice in sterile PBS (50µL ...
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bioRxiv - Immunology 2023Quote: NF-κB/AP-1 activation in THP-1-XBlue-CD14 reporter monocytes was determined after 20– 24 h of incubation according to the manufacturer’s protocol (InvivoGen, San Diego, USA). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added in order to generate a standard curve for each assay ...
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bioRxiv - Immunology 2022Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added to generate a standard curve for each assay ...
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bioRxiv - Genomics 2024Quote: ... or 30 ng/ml human recombinant TNF-α (rcyc-htnfa; Invivogen), respectively ...
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bioRxiv - Pathology 2020Quote: HEK-Blue™ Null1-v and HEK-Blue™-TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines were obtained from Invivogen (SanDiego, CA, USA). HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc. ...
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bioRxiv - Immunology 2022Quote: The following recombinant proteins were purchased and mixed with adjuvant (AddaVax, Invivogen) before injection into mice.
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Biophysics 2020Quote: Co-expression of FLAG- and HA-tagged UMOD isoforms was performed using a bicistronic vector pVITRO-hygro-mcs (Invivogen). For this purpose ...
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bioRxiv - Immunology 2023Quote: ... and S-29 groups were immunized with 15 μg of recombinant protein with AddaVaxTM (Invivogen) as adjuvant in the hock[49] ...
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bioRxiv - Immunology 2022Quote: ... mouse TLR2 neutralizing antibody monoclonal mouse IgG2a (C9A12) and control isotypes mouse IgG2a were from InvivoGen. Mouse TLR4/MD2 complex neutralizing antibody monoclonal rat IgGk clone MTS510 and control isotypes rat IgGk were from eBioscience™ Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: Wild-type and mutants MmEsco2-myc/his and H2B-mCherry were cloned into the pVITRO2-hygro-mcs vector (InvivoGen) in two steps ...
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bioRxiv - Microbiology 2020Quote: ... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... boost 4 weeks later of 5 μg recombinant spike protein (aa 14-1209) in 1% alhydrogel (Invivogen) into the right hind limb ...
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bioRxiv - Genomics 2024Quote: ... cells were stimulated for 45 minutes with 30 ng/ml human recombinant IL-1α (rcyec-hil1b; Invivogen) or 30 ng/ml human recombinant TNF-α (rcyc-htnfa ...
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bioRxiv - Immunology 2024Quote: ... and heavy and light chain variable sequences were synthesized as single chain variable fragments (ScFvs) containing a interchain (Light-Heavy) G4S3 linker and cloned into a pFuse-hG1-Fc2 Fc-tagged vector (InVivoGen, San Diego, CA), in-frame between the vector supplied signal sequence (from the human Il-2 protein ...
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bioRxiv - Microbiology 2021Quote: ... the mouse received a final boost intraperitoneally with 100 μg of recombinant N6 protein of A/duck/Zhejiang/D9/2013 (H4N6) adjuvanted with 10 μg of poly (I:C) (Invivogen). Three days after the final boost ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Pam3CSK4 (100 µg/mouse; InvivoGen), or Poly(I:C ...
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bioRxiv - Cell Biology 2023Quote: ... of the EVs and ENPs from EO771 m/p-mCherry or controls (medium alone, recombinant mCherry, LPS-EB ultrapure 10 µg/ml (Invivogen) and CpG 2 µg/ml (TriLink ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2022Quote: ... Mice in the RBD + aluminum hydroxide condition received 10 µg of recombinant monomeric SARS-CoV-2 RBD protein formulated with 100 µg of Alhydrogel adjuvant 2% (Invivogen). Mice in the PBS vaccination group received phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... B16-F10 cells expressing luciferase (B16-F10-luc) were generated by infection of recombinant lentivirus stably expressing luciferase luc2 under the control of hEF1A-eIF4G promoter (InvivoGen)61 ...
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bioRxiv - Bioengineering 2023Quote: ... 2×105 PBMCs and 4×104 autologous CD8+ T cells were incubated with either supernatants from engineered PCs or media containing recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) or anti-CD33 x anti-CD3 bispecific (AMG 330 ...
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bioRxiv - Bioengineering 2023Quote: ... 5×103 control cells and 5×104 CD8+ T cells were incubated with either various dilutions of supernatants from genome-engineered cells or media containing various concentrations of recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) for 48 hours (Figure 1F-H & 3G-I) ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of CK2α recombinant DNA encoding residues 1 to 336 was subcloned into an in-house knob-and-hole pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Immunology 2020Quote: ... ovalbumin (OVA) EndoFit (5 μg/mouse) and Alhydrogel adjuvant 2% (alum, 100 μg/mouse) were purchased from Invivogen; recombinant hemagglutinin (rHA ...
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bioRxiv - Microbiology 2020Quote: ... with 50 μl/mouse of AddaVax (Invivogen) as the adjuvant ...
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bioRxiv - Immunology 2023Quote: ... or Poly(I:C) (100 µg/mouse; InvivoGen) in 200 µl PBS for 24 h ...
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bioRxiv - Immunology 2023Quote: ... or the mouse kappa light chain plasmid (InVivoGen). The gBlock DNA and plasmids were cut with the specified enzymes ...
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bioRxiv - Immunology 2020Quote: The VRC01 LC-P2A-HC construct was PCR-amplified from engineered C57BL/6J mouse cDNA and cloned between the promoter and constant regions of a mouse IgG2b heavy chain expression vector (InvivoGen, #pfuse-mchg2b) using Gibson Assembly ...
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bioRxiv - Immunology 2019Quote: ... a Mouse TLR Agonist kit was purchased from Invivogen and BMDMs were treated with each ligand for 16 hours ...
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bioRxiv - Cell Biology 2022Quote: ... ASC-expressing RAW264.7 mouse macrophages (RAW-ASC; InvivoGen raw-asc) are engineered to stably express murine ASC ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies used: Mouse anti-HA antibody (InvivoGen, 1:1000), rabbit anti-ACP (1:2000) ...