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Citations for Invivogen :
1 - 50 of 151 citations for Recombinant Human TLR4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Human embryonic kidney (HEK) 293 reporter cells for TLR2 and TLR4 (Invivogen, France) were exposed to the eluted dust samples to study their potential to induce NFkB responses ...
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bioRxiv - Cell Biology 2021Quote: ... Targets for inhibition were TLR4 (anti-TLR4 IgG, 1 μg/ml; Invivogen); interleukin-1 receptor-associated kinase 1 and 4 (IRAK 1/4 ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Immunology 2020Quote: HEK293 cells expressing murine TLR2 or human TLR4 and CD14/MD2 were purchased from InvivoGen. Cells were cultured in the presence of blasticidin (Nacalai Tesque ...
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bioRxiv - Bioengineering 2021Quote: ... HEK-Blue™ TLR4 cells (Invivogen) were incubated with increasing concentrations of MPLA PS for 24 h 37 °C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... TLR4 (HEK-TLR4) and HEK-TLR2-blue and control HEK cell line (HEK-null) were purchased from InvivoGen and cultured in DMEM supplemented with 10 % FCS ...
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bioRxiv - Developmental Biology 2023Quote: ... HEK-Blue TLR4 cells (Invivogen, #hkb-htlr4) and HEK-Blue-mNOD2 cells (Invivogen ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Recombinant human TNF-α was purchased from InvivoGen. The antiretroviral drugs efavirenz ...
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bioRxiv - Immunology 2020Quote: ... TLR4 ligand lipid A (1 μg/ml, Invivogen), TLR7 ligand R-848 (30 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... and THP1-Dual-TLR4 KO Cells (all Invivogen) were maintained between 0.5-1×106 cells/ml in RPMI 1640 media ...
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bioRxiv - Immunology 2024Quote: Assessment of TLR4 and TLR5 activity was implemented using human embryonic kidney (HEK)-Blue-mTLR4 and HEK-Blue-mTLR5 cells respectively (Invivogen) using a protocol as previously described (Allen et al. ...
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bioRxiv - Immunology 2020Quote: ... LPS K12 (TLR4; tlrl-eklps; Invivogen, 200 ng/ml), Pam3CSK4 (TLR1/2 ...
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bioRxiv - Biochemistry 2022Quote: ... and ultrapure TLR4-specific LPS (Invivogen, San Diego CA). Flag-TRB3 adenovirus was generated as described 3) ...
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bioRxiv - Pathology 2020Quote: HEK-Blue™ Null1-v and HEK-Blue™-TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines were obtained from Invivogen (SanDiego, CA, USA). HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc. ...
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bioRxiv - Immunology 2020Quote: ... LPS 0111:B4 (TLR4; tlrl-3pelps; Invivogen, 200 ng/ml), LPS K12 (TLR4 ...
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bioRxiv - Immunology 2019Quote: ... Ultrapure TLR4-agonist (Salmonella Minnesota LPS) was purchased from InvivoGen. Recombinant human M-CSF was obtained from ImmunoTools ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added in order to generate a standard curve for each assay ...
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bioRxiv - Immunology 2022Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added to generate a standard curve for each assay ...
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bioRxiv - Genomics 2024Quote: ... or 30 ng/ml human recombinant TNF-α (rcyc-htnfa; Invivogen), respectively ...
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bioRxiv - Microbiology 2022Quote: ... The protocol using HEK-Blue™-hTLR2 and HEK-Blue™-hTLR4 reporter cell lines expressing human TLR2 and TLR4 (InvivoGen, San Diego, CA) was employed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... at 5 μg/mL or the TLR4 agonist lipopolysaccharide (LPS; Invivogen) at 100 ng/mL or the TLR9 agonist class A CpG ODNs (CpG-A ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Microbiology 2022Quote: The TLR4 signaling inhibitor (TAK-242; CLI-095) was purchased from Invivogen. It was dissolved at a concentration of 1 mg/ml in dimethyl sulfoxide and then 10x diluted with assay medium ...
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bioRxiv - Immunology 2022Quote: HEK-293 cells stably expressing TLR4/MD2/CD14 were purchased from Invivogen. Cells were seeded at 3.5 × 104 live cell/well in 96 well plates overnight and were transfected with NF-κB-luciferase reporter plasmid (50 ng/well ...
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bioRxiv - Immunology 2021Quote: ... the TLR4 antagonist LPS-RS (10 μg/mL, InVivoGen, San Diego, CA), the TLR1/TLR2 antagonist CU-CPT22 (20 μM ...
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bioRxiv - Cell Biology 2019Quote: Ultrapure biotin-LPS that activates only TLR4 pathway was purchased from InVivoGen. To analyze LPS binding ...
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bioRxiv - Systems Biology 2023Quote: ... TLR4 agonist at 4 ng/mL (LPS-B5, Invivogen, San Diego, CA), BTC at 40 ng/mL (human recombinant Betacellulin protein ...
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bioRxiv - Systems Biology 2020Quote: ... HEK 293 stable TLR4-MD2-CD14 cell line was also purchased from InvivoGen. All chemicals were HPLC-grade unless specifically indicated ...
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bioRxiv - Immunology 2020Quote: ... Lipopolysaccharide (LPS) from Escherichia coli and Salmonella minnesota (both ultrapure TLR4 agonists, InvivoGen); Lipoteichoic acid (LTA ...
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bioRxiv - Immunology 2020Quote: ... for TLR4 stimulation 1 µg/ml LPS Ultra pure (InvivoGen, San Diego, USA), and for TLR9 stimulation 5 µg/ml ODN1826 (tlrl-1826 ...
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bioRxiv - Immunology 2023Quote: ... and 1 ug/mL LPS (TLR4 agonist from E. coli O111:B4, InvivoGen)) for 6 hours ...
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bioRxiv - Immunology 2023Quote: ... and 1 ug/mL LPS (TLR4 agonist from E. coli O111:B4, InvivoGen)) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
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bioRxiv - Immunology 2021Quote: ... The TLR4 signaling inhibitor CLI-095) and MPLA were purchased from Invivogen (San Diego, CA). The MAPK inhibitor (PD98059) ...
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bioRxiv - Immunology 2021Quote: ... Some wells were pre-treated for 30 minutes with 10μg/ml TLR4 inhibitor TAK-242 (Invivogen). Cells were harvested after 4 days ...
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bioRxiv - Immunology 2023Quote: ... These analyses were conducted using either a HEK293T TLR4-CD14-MD2 IL-18 reporter line (Invivogen) or the RAW-Blue Cell assay (Invivogen) ...
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bioRxiv - Genomics 2024Quote: ... cells were stimulated for 45 minutes with 30 ng/ml human recombinant IL-1α (rcyec-hil1b; Invivogen) or 30 ng/ml human recombinant TNF-α (rcyc-htnfa ...
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bioRxiv - Systems Biology 2020Quote: ... and then cultured with medium or LPS (100 ng/mL, LPS-EB Ultrapure, activates TLR4 only, Invivogen) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... TLR4 activation was stimulated by adding lipopolysaccharide (LPS-EB from E. coli 0111:B4; InvivoGen, Toulouse, France) in a final concentration of 50 ng/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Purified proteins were tested for endotoxin via HEK-Blue TLR4 reporter cell line (Invivogen, San Diego, CA) and endotoxin levels were confirmed to be less than 0.01 EU/mL ...
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bioRxiv - Bioengineering 2021Quote: ... The purified proteins were tested for endotoxin using a HEK-Blue™ TLR4 reporter cell line (InvivoGen), and the endotoxin levels were confirmed to be below 0.01 EU mL−1 ...
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bioRxiv - Immunology 2021Quote: ... Specific wells were pre-cultured for 2 hours with either 10mg/ml of TLR4 inhibitor (CLI-095, Invivogen) or 10mg/ml PI3 Kinase inhibitor (LY294002 ...
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bioRxiv - Immunology 2020Quote: ... a component of the gram-negative bacterial membrane that binds to the TLR4 receptor were purchased from Invivogen.
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... as a measure of TLR4 activation in HEK-null2 and HEK-hTLR4 cells as recommended by the provider (Invivogen) Secreted cytokines were collected 48 hrs post-agonist treatment and quantified by ELISA following the manufacturer protocols ...
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bioRxiv - Immunology 2023Quote: ... was diluted in sterile endotoxin-free water. TLR3 agonist polyinosinic-polycytidylic acid - Poly(I:C) and TLR4 agonist LPS (Lipopolysaccharide from E. coli O55:B5) (Invivogen) were diluted in sterile endotoxin-free physiological water (NaCl 0.9%) ...
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bioRxiv - Neuroscience 2023Quote: ... LPS-RS Ultrapure (a selective TLR4 antagonist, lipopolysaccharide from Rhodobacter 9 sphaeroides, purchased from InvivoGen, San Diego, CA, USA), Collagenase Type 4 ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were verified as >95% pure by SDS-PAGE and contained <0.01 endotoxin units ml-1 by HEK-Blue TLR4 reporter assay (InvivoGen).
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2020Quote: ... TLR4 agonist: CRX-527 (Cat. #tlrl-crx-527) and TLR4 antagonist: LPS-RS Ultrapure (Cat.# tlrl-prslps) were obtained from InvivoGen. Human IL1RL1/ST2 Protein (isoform a ...