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Citations for Invivogen :
1 - 50 of 429 citations for Recombinant Human Interleukin 22 Receptor Alpha 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Recombinant human TNF-α was purchased from InvivoGen. The antiretroviral drugs efavirenz ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added in order to generate a standard curve for each assay ...
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bioRxiv - Immunology 2022Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added to generate a standard curve for each assay ...
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bioRxiv - Genomics 2024Quote: ... or 30 ng/ml human recombinant TNF-α (rcyc-htnfa; Invivogen), respectively ...
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bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
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bioRxiv - Genomics 2024Quote: ... cells were stimulated for 45 minutes with 30 ng/ml human recombinant IL-1α (rcyec-hil1b; Invivogen) or 30 ng/ml human recombinant TNF-α (rcyc-htnfa ...
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bioRxiv - Immunology 2022Quote: ... Mice in the RBD + aluminum hydroxide condition received 10 µg of recombinant monomeric SARS-CoV-2 RBD protein formulated with 100 µg of Alhydrogel adjuvant 2% (Invivogen). Mice in the PBS vaccination group received phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... a toll-like receptor 3 agonist (InvivoGen). Peptide doses of 50 ...
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bioRxiv - Molecular Biology 2020Quote: ... TNF-alpha (200 ng ml−1, Invivogen), IFN-beta (5 ng ml−1 ...
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bioRxiv - Microbiology 2020Quote: ... Human CD14+ monocytes (2×106/ml) were pre-incubated with human anti-Dectin-1 (10μg/ml, Invivogen), anti-TLR2 blocking antibodies (10μg/ml ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Microbiology 2021Quote: Agonists of pattern recognition receptors were purchased from Invivogen. Cells were seeded into 96 well plates and at 60% confluency ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen) and 10% FCS ...
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bioRxiv - Pathology 2020Quote: HEK-Blue™ Null1-v and HEK-Blue™-TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines were obtained from Invivogen (SanDiego, CA, USA). HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc. ...
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bioRxiv - Immunology 2020Quote: ... EV or Toll-like receptor–ligands (TLR-L) (InvivoGen, Toulouse, France): imiquimod (IMIQ ...
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bioRxiv - Biochemistry 2023Quote: ... Receptor constructs were subcloned into the pFuse-hIgG1-Fc2 vector (Invivogen) using EcoRI and BglII restriction sites ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Immunology 2021Quote: ... IgA+ cells were detected by addition of goat anti-human IgA F(ab’)2 (InVivoGen) followed by anti-goat IgG AlexaFluor 594 (Jackson Immunoresearch ...
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bioRxiv - Microbiology 2021Quote: Human IgA was purified from fecal supernatants with Peptide M/ Agarose (InvivoGen, Cat. No. gel-pdm-2) as described by the manufacturer ...
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bioRxiv - Microbiology 2019Quote: ... Miltenyi, 1000U/mL) and interleukin-4 (IL-4, Miltenyi, 1000U/mL) for 5 days before addition of lipopolysaccharide (LPS, Invivogen, 50 ng/mL) for 2 further days.
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Immunology 2023Quote: Cells were primed with 2 µg/mL (human primary Mo) or 10 µg/mL (BLaER1 Mo) of Pam3CSK4 (Invivogen) for 2 h before activation ...
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bioRxiv - Immunology 2020Quote: ... animals received naked plasmid DNA or isolated recombinant proteins (30 μg/animal of designated recombinant protein, coupled with/without alum adjuvant (40 μg/animal) Alhydrogel® (Invivogen; vac-alu-250).
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells that stably express human ACE2 (ACE2-293T) were cultivated in the presence of 2 μg/ml puromycin (Invivogen). Caco2 (human epithelial colorectal adenocarcinoma ...
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bioRxiv - Immunology 2023Quote: ... were immunized intramuscularly with 22 µmol purified nanoparticle immunogen in the presence or absence of AddaVax™ (InvivoGen) at weeks 0 ...
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bioRxiv - Microbiology 2020Quote: ... prepared from recombinant flagellin from Salmonella typhymurium (RecFLA-ST, Invivogen) in sterile water was also added to the plate in triplicate ...
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bioRxiv - Immunology 2022Quote: ... 40µg recombinant RBD plus 5µg LPS-EB VacciGrade™ (InvivoGen) was given to isoflurane anesthetized mice in sterile PBS (50µL ...
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bioRxiv - Immunology 2023Quote: ... with 50 μg of the Toll-like receptor (TLR)3 agonist polyinosinic-polycytidylic acid [poly (I:C)] (InvivoGen), 50 μg of the TLR7 agonist imiquimod (InvivoGen) ...
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bioRxiv - Immunology 2020Quote: ... a component of the gram-negative bacterial membrane that binds to the TLR4 receptor were purchased from Invivogen.
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bioRxiv - Immunology 2019Quote: ... was purified as previously described 22 5.6 ug of RTX was combined with 100 µl alum adjuvant (Alhydrogel®, InvivoGen) corresponding to 1 mg of aluminum hydroxide ...
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bioRxiv - Biochemistry 2021Quote: ... Stable MCF10A cells expressing these proteins were generated from MCF10A ARPIN −/− cells 22 transfected as described above and selected with 10 μg/ml Blasticidin (InvivoGen). Individual clones were picked with cloning rings.
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bioRxiv - Immunology 2022Quote: The following recombinant proteins were purchased and mixed with adjuvant (AddaVax, Invivogen) before injection into mice.
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bioRxiv - Immunology 2022Quote: ... and human IL-1β (Invivogen), were dissolved in PBS and added to the cell culture medium to achieve final concentrations of 10 μM (ADP-heptose) ...
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bioRxiv - Immunology 2023Quote: ... human p21 (Invivogen; 100 ng), human p27 (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... human p27 (Invivogen; 500 ng), and SV40 large T antigen (20 ng ...
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bioRxiv - Immunology 2023Quote: ... the human cDNA sequence (Invivogen) was subcloned into the pcDNA3 vector between the HindIII and EcoRV restriction sites followed by transfection of 5 µg plasmid into BAFF/CD40L double positive fibroblasts using Lipofectin reagent (Thermo) ...
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bioRxiv - Immunology 2020Quote: Biological activity of laminarin in LamOVA conjugates was analyzed by ELISA using a soluble murine Fc-Dectin-1a receptor (InvivoGen). Hypoallergenicity was analyzed in vitro as a measure of rat basophil leukemia cell (RBL-2H3 ...
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Immunology 2023Quote: ... To produce pseudoviruses expressing the S protein from different variants the following plasmids were used: Alpha (B.1.1.7) (InvivoGen, plv-spike-v2); Beta (B.1.351 ...
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bioRxiv - Biophysics 2020Quote: Co-expression of FLAG- and HA-tagged UMOD isoforms was performed using a bicistronic vector pVITRO-hygro-mcs (Invivogen). For this purpose ...
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bioRxiv - Immunology 2022Quote: cDNA encoding a truncated human ACE2 fused to human albumin was sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vector was transiently transfected into Expi293F cells in suspension (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... A549 lung carcinoma cells expressing human ACE2 and human TMPRSS2 (A549.ACE2+.TMPRSS2+; cat n° a549-hace2tpsa, Invivogen) were grown in DMEM/10% FBS supplemented with 100 µg/ml Normocin ...
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bioRxiv - Immunology 2023Quote: ... and S-29 groups were immunized with 15 μg of recombinant protein with AddaVaxTM (Invivogen) as adjuvant in the hock[49] ...
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bioRxiv - Immunology 2021Quote: ... In some assays human anti-RBD (Invivogen) of known antibody concentration was used as standard ...
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bioRxiv - Immunology 2022Quote: ... 2 ug/mL Pam3CSK4 (TLR1/2 agonist, Invivogen), and 1 ug/mL FSL-1 (TLR2/6 agonist ...
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bioRxiv - Molecular Biology 2019Quote: Wild-type and mutants MmEsco2-myc/his and H2B-mCherry were cloned into the pVITRO2-hygro-mcs vector (InvivoGen) in two steps ...
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bioRxiv - Microbiology 2020Quote: ... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...