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Citations for Invivogen :
51 - 100 of 107 citations for Recombinant Human IFNAR2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Human cGAS (hcGAS) and murine cGAS (mcGAS) plasmids were purchased from Invivogen. Flag-cGAS and V5-cGAS were amplified by PCR and were cloned into pcDNA3.2-DEST plasmids ...
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bioRxiv - Microbiology 2021Quote: ... generated from the human lung A549 cell line were purchased from InvivoGen SAS (Toulouse Cedex 4 ...
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bioRxiv - Bioengineering 2023Quote: ... 2×105 PBMCs and 4×104 autologous CD8+ T cells were incubated with either supernatants from engineered PCs or media containing recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) or anti-CD33 x anti-CD3 bispecific (AMG 330 ...
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bioRxiv - Bioengineering 2023Quote: ... 5×103 control cells and 5×104 CD8+ T cells were incubated with either various dilutions of supernatants from genome-engineered cells or media containing various concentrations of recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) for 48 hours (Figure 1F-H & 3G-I) ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of CK2α recombinant DNA encoding residues 1 to 336 was subcloned into an in-house knob-and-hole pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Immunology 2020Quote: Human embryonic kidney TLR5 knock-down cells (HEK-Blue-KD-TLR5 cells, Invivogen) were used for the in vitro experiments In these HEK-BLUE cells ...
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bioRxiv - Microbiology 2022Quote: Human embryonic kidney (HEK) 293 reporter cells for TLR2 and TLR4 (Invivogen, France) were exposed to the eluted dust samples to study their potential to induce NFkB responses ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with a human-mouse hybrid aCD20 (InvivoGen hcd20-mab10, 5 ng/ml), added to wells at 40,000 cells per well ...
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bioRxiv - Microbiology 2021Quote: ... X293T (Human Embryonic Kidney cell line) and HEK-Blue IFN α/β cells (InvivoGen) were cultured in Dulbecco’s modified eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... A549 lung carcinoma cells expressing human ACE2 (M08-0801, ©InvivoGen, San Diego, USA) were cultivated in DMEM (Gibco® ...
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bioRxiv - Microbiology 2021Quote: ... The 279bp human AldA enhancer was PCR amplified from the pDRIVE5-GFP-10 plasmid (Invivogen). The HCMV enhancer was PCR amplified from the pDrive5 GFP-1 Promoter Test (Invivogen) ...
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bioRxiv - Immunology 2021Quote: ... IgA+ cells were detected by addition of goat anti-human IgA F(ab’)2 (InVivoGen) followed by anti-goat IgG AlexaFluor 594 (Jackson Immunoresearch ...
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bioRxiv - Immunology 2020Quote: HEK293 cells expressing murine TLR2 or human TLR4 and CD14/MD2 were purchased from InvivoGen. Cells were cultured in the presence of blasticidin (Nacalai Tesque ...
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bioRxiv - Microbiology 2021Quote: ... Two days post-transfection the media was replaced with DMEM containing 0.5% fetal bovine serum and NOD1 or NOD2 agonist [1 μg/ml C12-iE-DAP (Invivogen)+ 10 ng/ml human interferon gamma (AbD serotec) and 5 μg/ml L-18-MDP (InvivoGen) + 10 ng/ml human interferon gamma ...
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bioRxiv - Immunology 2021Quote: ... WIL2-S cells were opsonized with dilutions of anti-human CD20 – murine IgG2a (hcd20-mab10, InvivoGen) for 30 min followed by incubation with the diluted secondary antibody for 30 minutes and washed 3 times with HBSS ...
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bioRxiv - Immunology 2020Quote: ... Treatment of live leptospires was performed with 100 μg/mL of the human cathelicidin LL37 (Invivogen) in PBS for 2 hours.
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bioRxiv - Cancer Biology 2022Quote: ... We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen, #ant-bl-1) for 14 days or with 1 mg ml-1 puromycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... human γδT cells were pre-treated with p-JNK inhibitor (SP600125: 20uM, Invivogen, San Diego, CA) for 30min followed by IL-12/18/21 stimulation for 4 hours(43) ...
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bioRxiv - Genetics 2020Quote: The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: Human IgA was purified from fecal supernatants with Peptide M/ Agarose (InvivoGen, Cat. No. gel-pdm-2) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were transfected with mscv2.2 vectors containing human or murine NLRC4 were using lipofectamine 2000 (Invivogen). Briefly ...
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bioRxiv - Genomics 2019Quote: ... supplemented with 5% human platelet lysate (Cook Regentec, #G34936) and 50 µg/mL Primocin (InvivoGen, #ant-pm-1). hASCs were cultured in the same media composition until 70-80% confluency and detached using TrypLE Select (Life Technology ...
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bioRxiv - Cell Biology 2022Quote: A549 lung carcinoma cells or A549 cells expressing human ACE2 or ACE2 and TMPRSS2 were obtained from Invivogen (a549 ...
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bioRxiv - Immunology 2020Quote: ... and 100 µl CpG (Murine CpG, ODN 1826, Human CpG ODN 2006 for pig and rabbit, InvivoGen, USA) were both added at 1mg/ml in WFI ...
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bioRxiv - Immunology 2019Quote: Detection of bioactive human IFN-I was performed using reporter cell line HEK-Blue IFN-α/β (InvivoGen) as previously described12 ...
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bioRxiv - Immunology 2021Quote: Human cell line THP-1 monocytes – ASC speck reporter cells were purchased from Invivogen (#thp-ascgfp, Invivogen, France) and called THP-1-ASC-GFP ...
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bioRxiv - Immunology 2023Quote: ... A549 with TMPRSS2 and human ACE2 overexpression cell line that was used in immunostaining was purchased from InvivoGen company (Cat.#a549-hace2tpsa ...
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Neuroscience 2022Quote: ... HEK-Blue™-hTLR2 cell line (Co-transfected with human TLR2 and SEAP reported gene) was purchased from InvivoGen. These cells were grown in DMEM media supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and MHC-I blocking antibody (W6/32; 50 ug/ml) in RPMI supplemented with human serum and primocin (Invivogen). Cells were harvested and stained with anti-CD3 FITC antibody (UCHT1 ...
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bioRxiv - Immunology 2020Quote: ... Anti-human IgG Fc Capture (AHC) Biosensors tips were initially loaded with Dectin-1A:FC fusion protein (Invivogen, #fc-hdec1a) at 13 ug/ml ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide ...
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bioRxiv - Immunology 2023Quote: Cells were primed with 2 µg/mL (human primary Mo) or 10 µg/mL (BLaER1 Mo) of Pam3CSK4 (Invivogen) for 2 h before activation ...
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bioRxiv - Microbiology 2023Quote: ... human cardiac fibroblasts in 96-well plate were transfected with 62 ng of high molecular weight poly(I:C) (InvivoGen) using TransIT-mRNA Transfection Kit (Mirus Bio) ...
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bioRxiv - Biochemistry 2023Quote: HKB11 PD-1 cells were used as stable pool generated by transfecting HKB11 cells with pMAX vector41 encoding human PD-1 (UniProt Q15116) and selection with 0.8 mg/mL geneticin (Invivogen).
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bioRxiv - Immunology 2019Quote: ... 293/NOD2 cells were obtained by stable transfection of HEK293 cells with the pUNO-hNOD2 plasmid which expresses the human NOD2 gene (Invivogen). THP1 cells were obtained from ATCC.
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bioRxiv - Microbiology 2021Quote: ... the human monocyte cell line THP1-Blue expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen) were cultured in RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Soluble Fc-mDectin-1a containing the C-terminal extracellular domain of mouse Dectin-1a fused with the human IgG1 Fc domain was purchased from Invivogen. Overnight C ...
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bioRxiv - Cancer Biology 2022Quote: ... vector with the KIH strategy “knob” human Fc domain(54) MLA2-arm of the TriTE was converted into a scFab and cloned into a pFUSE (InvivoGen) vector with the KIH strategy “hole” human Fc domain followed by OKT3 ...
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bioRxiv - Microbiology 2020Quote: The DNA sequences of codon-optimized SARS-CoV-2 spike Receptor Binding Domain (S-RBD) and human ACE2 extracellular domain (hACE2-ECD) were cloned into a pFuse-Fc expression vector (Invivogen). A thrombin cleavage sequence was inserted between RBD and Fc to generate a cleavable human Fc tag for future studies ...
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bioRxiv - Bioengineering 2022Quote: Non-glycosylated monoclonal human IgG1 antibody against human EGFR (hegfr-mab12, lot: EG12-39-01) and isotype control -Human IgG1 (bgal-mab1, lot: BG1-41-01) were purchased from InvivoGen. Human EGFR (Research Grade Cetuximab Biosimilar ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Human Embryonic Kidney HEK293-Null2 cells (Cat #hkb-null2) and HEK293-hTLR4 cells (Cat #hkb-htlr4) were obtained from Invivogen and are isogenic reporter cell lines ...
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bioRxiv - Microbiology 2021Quote: Human embryonic kidney HEK293T/17-hACE2 cells with stable expression of human angiotensin-converting enzyme ACE2 (AXON Neuroscience SE) were prepared by stable transfection of pDUO2-hACE2-TMPRSS2a (InvivoGen) with transfection reagent Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells that stably express human ACE2 (ACE2-293T) were cultivated in the presence of 2 μg/ml puromycin (Invivogen). Caco2 (human epithelial colorectal adenocarcinoma ...
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells stably expressing human TMPRSS2 were generated by lentiviral transduction followed by selection with blasticidin (40 μg/mL; Invivogen). SARS-CoV-2 strains hCoV-19/USA-WA1/2020 (NR-52281) ...
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bioRxiv - Molecular Biology 2023Quote: ... was fused to the Fc region (CH2 and CH3 domains) and hinge region of the human IgG1 heavy chain in the pFUSE-Fc1 vector (InvivoGen). Jag1ECD-Fc protein was expressed in Expi293F cells grown in Expi293F expression medium at 37°C in an 8% CO2 incubator with constant shaking ...
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bioRxiv - Immunology 2023Quote: The human monocytic THP-1 cell line (ATCC TIB-202) and the THP-1 DualTM reporter cell (InvivoGen, Thpd-nfis) were culture in RPMI supplemented with 10% heat-inactivated fetal calf serum ...
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bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
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bioRxiv - Immunology 2024Quote: Assessment of TLR4 and TLR5 activity was implemented using human embryonic kidney (HEK)-Blue-mTLR4 and HEK-Blue-mTLR5 cells respectively (Invivogen) using a protocol as previously described (Allen et al. ...