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Citations for Invivogen :
201 - 230 of 230 citations for PD L2 Cynomolgus HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: All materials for in vivo use were tested for endotoxin using the HEK-Blue mTLR4 reporter cell line (InvivoGen, San Diego, CA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Parent Flp-In™ T-REx™-293 cells were additionally supplemented with 15 μg/ml blasticidin (InvivoGen, cat n°ant-bl-1) and 100 μg/ml zeocin for cultivation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... we used engineered HEK293 cell lines (HEK-Blue) that stably express either mouse TLR9 (mTLR9) and an NF-κB reporter gene (Invivogen, San Diego, CA). In brief ...
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bioRxiv - Pathology 2020Quote: HEK-Blue™ Null1-v and HEK-Blue™-TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines were obtained from Invivogen (SanDiego, CA, USA). HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc. ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were allowed to adhere for 24 hours prior to removal of growth medium from each well and subsequent addition of 160uL of prewarmed HEK-Blue Detection Medium (Invivogen, cat. code hb-det). 40uL of NANP sample was added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... 20μl of HEK cell supernatant was transferred to a flat-bottom 96-well plate containing 180 μl of QuantiBlue (Invivogen, cat. code rep-qbs) detection reagent and incubated at 37°C for 30 minutes ...
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bioRxiv - Bioengineering 2020Quote: Nanobodies targeting RBD were expressed and secreted as Fc fusions by cloning into pFUSE-hlgG1-Fc2 (Invivogen) using the NcoI and EcoRI restriction sites or by Gibson assembly ...
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bioRxiv - Biophysics 2019Quote: The coding sequences of antibody Fc heterodimerization domains were amplified from the pFUSE-hIgG1-Fc1 plasmid (Invivogen) containing two charged mutations on each opposing domain57,58 ...
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bioRxiv - Immunology 2019Quote: ... before being cultured for indicated time points in FCS-free medium containing 1 µg/ml LPS (Invivogen), 1 μg/ml synthetic (B ...
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bioRxiv - Cell Biology 2021Quote: Endogenously tagged cell lines were maintained in HMI-9 supplemented with 20 % (v/v) FCS at 37 °C in 5% C02 and maintained in 10 µg.mL-1 Blasticidin (InvivoGen). Parasite density was monitored using a haemocytometer.
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bioRxiv - Microbiology 2022Quote: ... PCR products were gel purified and cloned into heavy and kappa expression plasmids52,53 adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 ampicillin resistant vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the mouse hinge and IgG2a heavy chain Fc region (pFUSE-mIgG2a-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide and was subcloned into a pMSGV retroviral vector followed by enhanced (e)GFP reporter gene cassette using PCR and standard molecular cloning techniques to generate pMSGV-A4-Fc-T2A-eGFP ...
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bioRxiv - Immunology 2023Quote: ... and 10% FCS in a 96 well ‘U’ bottomed plate and stimulated with 1μg/mL LPS-EB Ultrapure (Invivogen) and Brefeldin A (eBioScience) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were incubated for 30 min at room temperature (RT) and 0.2 µg/ml of Fc-h-dectin-hFc (Invivogen) was added to the UV-irradiated conidia and incubated for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: Biological activity of laminarin in LamOVA conjugates was analyzed by ELISA using a soluble murine Fc-Dectin-1a receptor (InvivoGen). Hypoallergenicity was analyzed in vitro as a measure of rat basophil leukemia cell (RBL-2H3 ...
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bioRxiv - Biochemistry 2020Quote: ... 17 ECD-10His (extracellular domain) was generated by Gibson cloning and adapted from the commercially available pFUSE-hIgG1-Fc (InvivoGen) vector ...
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bioRxiv - Microbiology 2020Quote: ... Soluble Fc-mDectin-1a containing the C-terminal extracellular domain of mouse Dectin-1a fused with the human IgG1 Fc domain was purchased from Invivogen. Overnight C ...
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bioRxiv - Cancer Biology 2022Quote: ... vector with the KIH strategy “knob” human Fc domain(54) MLA2-arm of the TriTE was converted into a scFab and cloned into a pFUSE (InvivoGen) vector with the KIH strategy “hole” human Fc domain followed by OKT3 ...
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bioRxiv - Microbiology 2020Quote: The DNA sequences of codon-optimized SARS-CoV-2 spike Receptor Binding Domain (S-RBD) and human ACE2 extracellular domain (hACE2-ECD) were cloned into a pFuse-Fc expression vector (Invivogen). A thrombin cleavage sequence was inserted between RBD and Fc to generate a cleavable human Fc tag for future studies ...
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bioRxiv - Immunology 2019Quote: ... KX571250-1324) were cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... three BALB/c mice were each injected intraperitoneally (i.p.) with 100 μg of RBD-Fc fusion protein formulated with 500 μg of aluminum hydroxide (Alhydrogel, Invivogen, USA) and 25 μg of CpG (Sangon ...
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bioRxiv - Immunology 2020Quote: Total spleen cells were cultured at a density of 1 × 106 cells/mL in RPMI 1640 supplemented with 10% fetal calf serum (FCS) with 5 ng/mL LPS (Invivogen). For 4-hydroxytamoxifen (OHTAM ...
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Immunology 2023Quote: ... These Gibson sequences are necessary for cloning of the VH and VL into our rabbit IgG1 expression vectors which are based on the pFUSE-rIgG-Fc (InvivoGen) vectors.75,76 For this second PCR ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of recombinant DNA encoding either residues 21 to 220 of human CD44 or 25 to 224 of murine CD44 were subcloned into an in-house Fc pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... and heavy and light chain variable sequences were synthesized as single chain variable fragments (ScFvs) containing a interchain (Light-Heavy) G4S3 linker and cloned into a pFuse-hG1-Fc2 Fc-tagged vector (InVivoGen, San Diego, CA), in-frame between the vector supplied signal sequence (from the human Il-2 protein ...