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Citations for Invivogen :
151 - 200 of 289 citations for IL 13 Human HEK 293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... THP1 cells were treated with phorbol 12-myristate 13-acetate (PMA, 10 ng/ml; Invivogen) overnight prior to infection ...
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bioRxiv - Bioengineering 2021Quote: ... Purified proteins were tested for endotoxin via HEK-Blue TLR4 reporter cell line (Invivogen, San Diego, CA) and endotoxin levels were confirmed to be less than 0.01 EU/mL ...
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bioRxiv - Immunology 2021Quote: The capacity of E protein to activate NF-kB was tested by using HEK-TLR2-blue (InvivoGen). In this assay ...
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bioRxiv - Bioengineering 2021Quote: ... The purified proteins were tested for endotoxin using a HEK-Blue™ TLR4 reporter cell line (InvivoGen), and the endotoxin levels were confirmed to be below 0.01 EU mL−1 ...
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bioRxiv - Microbiology 2022Quote: ... HEK-293T-hACE2 cells were grown in complete DMEM medium supplemented with zeocin (50 μg/mL; InvivoGen) (Glowacka et al. ...
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bioRxiv - Bioengineering 2023Quote: The RIG-I reporter cell line HEK-Lucia™ RIG-I cells (Catalog Code, hkl-hrigi, InvivoGen) and the control cell line HEK-Lucia™ Null cells (Catalog No ...
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bioRxiv - Immunology 2023Quote: The agonistic activity of anti-CD40 monoclonal antibodies was tested by stimulating HEK-Blue-CD40L™ (Invivogen) cells which harbor an NF-κB-inducible Secreted Embryonic Alkaline Phosphatase (SEAP ...
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bioRxiv - Microbiology 2023Quote: ... DMEM growth medium was supplemented with 100 µg/ml normocinTM and/or 1X HEK-BlueTM Selection (Invivogen) where appropriate.
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bioRxiv - Bioengineering 2022Quote: ... 293-cov2-sdf) or 2) Delta/B.1.617.2 variant) (Catalogue code: 293-SARS2-S-V8-dfur) were purchased from Invivogen. The cells were grown in DMEM media containing 10% Fetal Bovine Serum ...
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bioRxiv - Immunology 2019Quote: ... Cells were confirmed to be free of Mycoplasma infection using the Hek Blue Mycoplasma Detection Kit from Invivogen.
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bioRxiv - Microbiology 2023Quote: HEK-BlueTM hTLR2 secreted alkaline phosphatase (SEAP) reporter assays were performed according to manufacturer instructions (Invivogen, hkb-htlr2). Briefly ...
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bioRxiv - Bioengineering 2024Quote: The ability of 1V209-modified peptides to activate TLR7 was assessed using HEK-Blue mTLR7 reporter cells (InvivoGen) using the standard protocol provided by the supplier ...
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bioRxiv - Immunology 2024Quote: ... Cell lines were regularly checked for Mycoplasma contamination using the cell- based colometric HEK-Blue detection assay (Invivogen) and passaged maximally 20 times ...
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bioRxiv - Immunology 2022Quote: ... Freestyle™ 293-F were transfected with pUNO1-Spike-dfur expression vectors (Spike and SpikeV1 to V11 plasmids, Invivogen) (1.2 µg plasmid DNA per 106 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... To analyze the interferon response HEK-Dual hTLR3 cells were treated with 5 μg/ml poly(I:C) HMW (InvivoGen) or the different extracted media for four hours ...
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bioRxiv - Immunology 2021Quote: ... Half-area tissue-culture-treated 96 well plates were filled with 10μl of each diluted sample followed by 90μl of the reporter cell lines suspended in HEK-Blue Detection Media (Invivogen). Assays were performed with cell densities of 1.4 x 105 cells/well (mTLR2 ...
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bioRxiv - Biochemistry 2019Quote: ... Supernatant from HEK-Blue IFN-α/β cells was then assayed for alkaline phosphatase activity using QUANTI-Blue (InvivoGen), following manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... HeLa EGFP-CHIP and HEK EGFP-CHIP cells were supplemented with blasticidin (10 µg/ml) (ant-bl-1, Invivogen) and hygromycin B (50 µg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... endotoxin levels in exosomes and iPS-Mg medium were measured using the HEK Blue LPS detection kit 2 (InvivoGen) following manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... detached with a cell scraper and resuspended at a density of 2.8 × 105 cells/mL in HEK-Blue Detection media (Invivogen). Neutralizing antibodies against TLR2 ...
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bioRxiv - Microbiology 2023Quote: ... 20 µl UV-treated sample was inoculated onto 4×104 cells/ well of HEK-Blue IFNα/β cells (InvivoGen) in 96-well plates ...
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bioRxiv - Molecular Biology 2023Quote: ... Type I Interferon (IFN I) levels were determined using the reporter cellline HEK-Blue™IFN-α/β (INVIVOGEN) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were verified as >95% pure by SDS-PAGE and contained <0.01 endotoxin units ml-1 by HEK-Blue TLR4 reporter assay (InvivoGen).
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bioRxiv - Immunology 2023Quote: ... FreeStyleTM 293-F cells were co-transfected with the specified M2e-MAbs heavy and light chain variable region plasmids (InvivoGen) using Polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell lines were maintained and Mycoplasma absence was tested regularly using the PlasmoTest kit (HEK blue, Invivogen, Toulouse, France). HCT116 NCLX KO cells are the same cells described in Pathak et al. ...
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bioRxiv - Immunology 2020Quote: ... the activation of NF-κB drives the expression of the alkaline phosphatase enzyme that induces a color shift from pink to blue of the chromogenic substrate in the HEK-Blue Detection Media (Invivogen). These cells were cultured in complete DMEM medium composed of DMEM GlutaMAX (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... transient transfection of HEK-293FT cells with packaging and lentiviral vector plasmids were performed using LENTI-Smart INT kit (InvivoGen) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: Bioactive functional type I IFN was quantified in supernatants using the reporter cell line HEK-Blue IFN-α/β (Invivogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... NF-κB activity was monitored by the production of secreted embryonic alkaline phosphatase (SEAP) through the hydrolysis of HEK-Blue (InvivoGen). Optical density was read at 650 nm using a SpectraMax340 PC plate reader (Molecular Devices).
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bioRxiv - Immunology 2023Quote: ... human p21 (Invivogen; 100 ng), human p27 (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... human p27 (Invivogen; 500 ng), and SV40 large T antigen (20 ng ...
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bioRxiv - Immunology 2023Quote: ... the human cDNA sequence (Invivogen) was subcloned into the pcDNA3 vector between the HindIII and EcoRV restriction sites followed by transfection of 5 µg plasmid into BAFF/CD40L double positive fibroblasts using Lipofectin reagent (Thermo) ...
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bioRxiv - Immunology 2021Quote: ... 8 x 104 U937 cells per well of a 96 wells plate were exposed to 100 ng.mL-1 of phorbol 12-myristate 13-acetate (PMA; InvivoGen) for 48 h and primed with LPS at 1 μg.mL-1 for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte THP1 cells (TIB2002, ATCC) were differentiated in macrophages with phorbol-12 myristate-13-acetate (PMA) (#tlrl-pma, Invivogen), as previously described (80 ...
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bioRxiv - Microbiology 2024Quote: ... and diluted to 2.5 x 105 cells/ml in complete RPMI with 100 nM phorbol 12-myristate 13-acetate (PMA) (InvivoGen) to differentiate cells to a macrophage phenotype ...
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bioRxiv - Immunology 2021Quote: ... HEK-Blue TLR3 and HEK-Blue TLR4 reporter cells (Cat. no.# hkb-htlr2, hkb-htlr3 and hkb-htlr4) were purchased from InvivoGen (USA). Human monocytic cells ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μL of the supernatant or an IFNα/β standard containing purified IFNα and IFNβ were added to 180 μL HEK-Blue cells (Invivogen) (50,000 cells ...
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bioRxiv - Immunology 2022Quote: ... cells were at 80 % confluence and the supernatant was discarded and replaced with 23 μL of control solutions (HEPES and water) or stimulants diluted in HEPES and water and 77 μL of HEK-Blue™ Detection medium (InvivoGen) (cell culture medium for real-time detection of SEAP) ...
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bioRxiv - Microbiology 2020Quote: ... 30 μl of HEK-Blue™ IFN-α/β supernatants was added to 120 uL of Quanti-blue™ substrate (InvivoGen) and incubated at 37°C for 15 min ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Bioengineering 2024Quote: All materials were verified to be free of endotoxins using the HEK-Blue mTLR4 reporter cell line (InvivoGen, San Diego, CA) using the protocol provided by the supplier ...
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bioRxiv - Microbiology 2022Quote: ... 293-ACE2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 200 µg/mL G418 (Invivogen #ant-gn-2). HEK-Blue 293 hACE2-TMPRSS2 cells (Invivogen #hkb-hace2tpsa ...
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bioRxiv - Synthetic Biology 2022Quote: ... IFN-λ2 [Cat#8417-IL-025/CF]) and IFN-α2 from InvivoGen [Cat# rcyc-hifna2b] were used as controls ...
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bioRxiv - Immunology 2019Quote: ... PIGTKO and wild-type THP-1 cells were differentiated into adherent macrophages in complete RPMI 1640 medium containing 100 ng/ml phorbol 12-myristate 13-acetate (PMA; InvivoGen) for 3 hr ...
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bioRxiv - Cell Biology 2022Quote: ... by stimulating cells with a range of concentrations (25 nM – 200 nM) of phorbol 12-myristate 13-acetate (PMA; InvivoGen) for different incubation periods ...
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bioRxiv - Immunology 2020Quote: ... 5 x 104 cells were directly sorted in 200 μl RPMI complete medium and stimulated for 24 hours in the presence of phorbol 12-myristate 13-acetate (PMA, 20ng/ml, Invivogen) and ionomycin (1μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... at 37 °C in 5 % CO2 and differentiated into macrophage-like phenotype cells by adding 100 nM of phorbol 12-myristate 13-acetate (PMA; InvivoGen) two days prior infection by ZIKV.
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bioRxiv - Immunology 2021Quote: THP-1 cells were cultured in culture medium containing final concentration of 100 ng/mL of phorbol-12-myristate 13-acetate (PMA; tlrl, Invivogen). Following 24h post PMA treatment ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl of supernatants from the HEK-Blue™ or THP1-Blue™ cells were mixed with 180 μl of QuantiBlue reagent (InvivoGen) and optical density (O.D. ...
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bioRxiv - Microbiology 2022Quote: ... 180 µl/well of cells at a concentration of 2.8 × 105 cells/ml in HEK-Blue detection media (Invivogen; catalog #hb-det2) were plated in 96 well tissue-culture plate ...