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Citations for Invivogen :
451 - 490 of 490 citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Guide-RNAs were stably transduced in SpCas9-expressing DC 2.4 cells using the LentiGuide-Neo vector and selected with 500 ug/mL of G418 (InvivoGen #108321-42-2). LentiGuide-Neo was a gift from Caroline Goujon (Addgene plasmid # 139449 ...
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bioRxiv - Cell Biology 2023Quote: ... and if required relevant selection antibiotics (hygromycin 200 µg/ml ; InvivoGen, ant-hg-1 ; puromycin 2 µg/ml, InvivoGen, ant-pr-1). Im95m cells were routinely maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Intraperitoneal sensitization: Mice were immunized intraperitoneally with 250 μg OVA and 250 μL Alhydrogel 2% (aluminum hydroxide [alum], InvivoGen, San Diego, CA), 40 μg CpG ...
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bioRxiv - Microbiology 2019Quote: ... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
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bioRxiv - Immunology 2022Quote: ... immunogen suspensions were gently mixed 1:1 vol/vol with AddaVax adjuvant for immunizations 1 and 2 and O/W for immunization 3 (Invivogen, San Diego, CA) to reach a final concentration of 0.250 mg/mL antigen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The expression vector containing SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...
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bioRxiv - Immunology 2020Quote: Undifferentiated THP-1 and PMA-differentiated cells (2 × 105 cells) in biological duplicates per condition were stimulated with TLR agonists- CL075 (TLR8; tlrl-c75; Invivogen, 5 μg/ml), CpG2006 (TLR9 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Neuroscience 2019Quote: ... 400 μg of conjugate fentanyl-TT hapten was mixed with 600 μg CpG ODN 2006 (Eurofins Genomics, Louisville, KY) and 1 mg Alhydrogel adjuvant 2% (InvivoGen, San Diego, CA) for 30 min and then refrigerated for 24h prior to IM administration at approximately 1.2 ml per monkey ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS cells were transfected with the targeting vectors and incubated for 2 days before addition of selective medium containing 1.5 μg/ml puromycin (Invivogen, cat# ant-pr-1). After 5 days ...
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bioRxiv - Immunology 2022Quote: ... Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen, San Diego, US) added at alternative passages ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The expression vector containing the SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Cell Biology 2021Quote: ... All cells were routinely checked for mycoplasma contamination using PlasmoTest™ Kit (InvivoGen).
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bioRxiv - Immunology 2024Quote: ... ODN1826 and ODN2395 were from a mouse TLR9 agonist kit (Invivogen, tlrl-kit9m). Poly(I:C ...
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bioRxiv - Plant Biology 2021Quote: ... Transgenic Col-0 (LUC) plants were selected on 1/2 MS medium supplemented with 50 μM hygromycin B (InvivoGen, Cat No. ant-hg-5). LUC activity was confirmed as described above ...
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bioRxiv - Pathology 2020Quote: HEK-Blue™ Null1-v and HEK-Blue™-TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines were obtained from Invivogen (SanDiego, CA, USA). HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... All cells were tested for mycoplasma contamination every two weeks with PlasmoTest kit (InvivoGen). Reagents used for transfections were ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected and secreted Luciferase activity assayed using QUANTI-Luc™ kit (InVivoGen) as per manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and the QUANTI-Blue™ assay kit were obtained from Invivogen (San Diego, CA). BrightGLO™ was purchased from Promega (Madison ...
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-PATC148 cells were obtained from MD Anderson Cancer Center and cultured in RPMI media supplemented with 2 uM glutamine and containing 100ug/mL primocin (Invivogen, Cat. no. ant-pm-1). Cell lines were routinely tested for mycoplasma and validated using STR profiling and mutational analysis.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... including testing for mycoplasma contamination with the Plasmotest™ kit (InvivoGen, San Diego, CA, USA) before freezing the cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Mycoplasma testing is done bimonthly using the MycoStrip 100 kit (InvivoGen, cat#: rep-mysnc-100).
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... Regular mycoplasma testing was performed using the MycoStrip Mycoplasma Dectection Kit (InvivoGen #rep-mys-50).
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bioRxiv - Bioengineering 2023Quote: ... 4 or 8 µg/ml) or ss-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (InvivoGen, Catalog Code. lyec-12, InvivoGen) following the manufacturer’ ss instructions ...
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bioRxiv - Pathology 2020Quote: ... a truncated HSP27 peptide consisting of the C-terminal half of the peptide, (100 µg, 75 µL) mixed with an aluminum hydroxide gel adjuvant (Alhydrogel® 2%, Al, 25 µL; Invivogen vac-alu-250, San Diego, CA). One week after the commencement of E2 and/or vaccinations ...
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bioRxiv - Immunology 2022Quote: ... Omicron BA.1 or Omicron BA.2 Spike expression vectors lacking the 19 C-terminal amino acids containing the ER-retention signal (InvivoGen plv-spike-v11 and plv-spike-v12). HEK293T cells (1.2×106 cells ...
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bioRxiv - Immunology 2022Quote: ... Luciferase presence in the supernatant was quantified by kit (QUANTI-luc, InvivoGen, catalog number rep-qlc1). Each sample was tested in triplicate in white 96-well plates with clear bottoms (LUMITRAC 200 ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was analysed using the Dead Cell Apoptosis Kit for Flow Cytometry from Invivogen (#V13242), with early apoptotic cells scoring Annexin V-positive and late apoptotic cells scoring Annexin V- and propidium iodide (PI)-positive ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: THP1-Blue™ NF-κB cells (derived from the human THP-1 monocyte cell line by stable integration of an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen, San Diego, CA, USA; 2 × 105 per well) were cultured in 96 well plate using RPMI containing 10 % heat inactivated FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The absence of mycoplasma was verified on a bimonthly basis using the PlasmoTest kit (Invivogen, cat. code rep-pt1).
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bioRxiv - Molecular Biology 2023Quote: ... Recombination cloning-compatible U2OS cells were generated using the Flp-In T-Rex Core Kit (Thermo) according to the manufacturer’s instructions and maintained under selection using Blasticidin (Invivogen) and Zeocin (Thermo) ...
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bioRxiv - Microbiology 2024Quote: ... or SuperScript™ III Platinum™ One-Step qRT-PCR Kit for TaqMan reactions (InvivoGen, San Diego, CA, USA). An RT reaction for 30 minutes at 50°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lines were tested for mycoplasma contamination once every two months using the Mycotest kit (InvivoGen, rep-mys-10).
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bioRxiv - Genetics 2020Quote: ... Cells were lysed using passive lysis buffer and the activity of the enhancer assessed using a Lucia QUANTI-Luc Gold Assay #3 luciferase kit (InvivoGen). Because of clear evidence of the response of the pGL4.74 Renilla normalisation control plasmid to PMA treatments and co-transfection with the EGR1 expression vector Lucia activity levels were normalised against quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell lines were maintained and Mycoplasma absence was tested regularly using the PlasmoTest kit (HEK blue, Invivogen, Toulouse, France). HCT116 NCLX KO cells are the same cells described in Pathak et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... transient transfection of HEK-293FT cells with packaging and lentiviral vector plasmids were performed using LENTI-Smart INT kit (InvivoGen) following manufacturer’s recommendations ...
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
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bioRxiv - Systems Biology 2023Quote: Hela-S3 cells were purchased from ATCC and cultured in DMEM supplemented with 10 % heat inactivated FBS without antibiotics and incubated at 37 °C and 5 % CO2 Cells were tested regularly for mycoplasma with the PlasmoTest kit (InvivoGen) and are mycoplasma negative ...