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Citations for Invivogen :
401 - 431 of 431 citations for Human integrin alpha 5 beta 3 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... C57BL/6 mice were immunized with Spike protein (5 µg, Bio-Techne) in presence of CpG adjuvant (10 µg, ODN1826, Invivogen) according to the immunization protocol in Figure 1 ...
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bioRxiv - Microbiology 2024Quote: ... Cells transduced with lentiviruses encoding for the different gRNAs were passaged in selection medium containing 10 µg/ml blasticidin and 5 µg/ml puromycin (ant-pr-1, InvivoGen) to obtain polyclonal cell populations lacking either GSDMD or GSDME ...
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bioRxiv - Cancer Biology 2024Quote: ... 24-well or 96-well plates at a concentration of 5×105 cells/mL in RPMI 1640 supplemented with 20% fetal calf serum and 1X primocin (Invivogen).
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells (5 × 104 cells/well) were co-cultured with 0.5 ng/mL SIINFEKL peptide (InVivoGen, # vac-sin)-pulsed splenocytes (5 × 104 cells/well ...
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bioRxiv - Immunology 2021Quote: ... Mice (8 mice per group) were intramuscularly injected twice at four-week intervals with each VLPs (HA content=3 µg) with AddaVax™ adjuvant (Invivogen, San Diego, CA, USA) (Figure 2) ...
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bioRxiv - Immunology 2019Quote: ... We also administered either a 0.9% saline or a 5 mg/kg dose of LPS that is TLR4-specific (InvivoGen, tlrl-3pelps) by i.p ...
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bioRxiv - Cancer Biology 2022Quote: B-ALL cells were infected with Cas9-2A-blast lentivirus followed by one-week selection with 5-15 µg/mL blasticidin (InvivoGen, CA). Cells were amplified and tested for Cas9 activity using the reporter assay described in Tzelepis et al.14 Pools with >75% Cas9 activity were used going forward ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Omicron Variant (B.1.1.529/BA.1) pLV-SpikeV11) and Omicron Variants (BA.4/BA.5) pLV-SpikeV13) were purchased from InvivoGen (San Diego, CA). The human T-Cell lymphoma Jurkat (E6-1 ...
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bioRxiv - Immunology 2023Quote: ... we immunized three groups of C57BL/6 mice (n=9) with 5 µg protein antigens adjuvanted with 500 µg alum (Alhydrogel®, Invivogen) and 20 µg CpG (ODN 1826 ...
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bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) Primocin™ (InvivoGen, San Diego, CA; ant-pm-1) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Human monocytes THP–1 cells were seeded at a density of 3×105 cells/ml and treated with 100 ng/ml phorbol myristate acetate (PMA, InvivoGen, San Diego, CA, Cat. No. tlrl-pma) for 24 hours for their differentiation to nascent (M0 ...
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bioRxiv - Molecular Biology 2020Quote: ... H1299 cells were transduced by the lentivirus vectors and selected with DMEM containing 5 µg/ml puromycin (InvivoGen, San Diego, CA, USA).
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bioRxiv - Genomics 2020Quote: ... The action of MAPK Kinases (MEK1 and MEK2) was blocked by the use of a selective inhibitor UO126 at 5 µM (Invivogen, CA, USA). The involvement of the TLR1/2 in signal transduction was tested using CU-CPT22 at 10 µM (Tocris Bioscience ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... hDaxx or ATRX were maintained in HFF cell culture medium additionally supplemented with 5 μg/ml puromycin (InvivoGen, San Diego, CA, USA). HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L Glucose, 1 mM Sodium Pyruvate, 10% FBS (Thermo Fischer, Massachusetts, US) and 100 μg/ml Normocin (Invivogen, California, US). Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 h the medium was replaced with a selection medium supplemented with 500 μg/ml hygromycin (Invivogen. Cat# ant-hg-5). The cells that survived and proliferated in 500 μg/ml hygromycin were selected and were permanently cultured in a medium containing 500 μg/ml hygromycin ...
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bioRxiv - Immunology 2023Quote: ... 1×106 UCBMC were stimulated for 6 hours at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 0.5 μg/ml PMA and 5 μg/ml ionomycin (InvivoGen, San Diego, California). CD107a antibodies were added at the beginning of stimulation ...
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bioRxiv - Biochemistry 2023Quote: ... Selection and maintenance of cells that underwent recombination started 24 h later by including hygromycin (50 µg/ml, Invivogen ant-hg-5) in the culture media ...
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bioRxiv - Microbiology 2019Quote: ... Miltenyi, 1000U/mL) and interleukin-4 (IL-4, Miltenyi, 1000U/mL) for 5 days before addition of lipopolysaccharide (LPS, Invivogen, 50 ng/mL) for 2 further days.
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bioRxiv - Immunology 2020Quote: Undifferentiated THP-1 and PMA-differentiated cells (2 × 105 cells) in biological duplicates per condition were stimulated with TLR agonists- CL075 (TLR8; tlrl-c75; Invivogen, 5 μg/ml), CpG2006 (TLR9 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Pathology 2020Quote: ... All constructs generated were stably transduced into previously CLCN5 silenced cells using lentiviral particles produced in HEK-293 cells and were subsequently selected with 400 μg/ml hygromycin (Invivogen, #ant-hg-5). Site-directed mutagenesis was performed with the QuikChange® II XL Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transduced with retrovirus in the presence of 6 μg/ml protamine sulfate and selected with 5 ug/ml Blasticidin (InvivoGen #ant-bl-05) for 5 days.
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bioRxiv - Cancer Biology 2022Quote: ... The final product was verified with Sanger sequencing from both directions and incorporated into cells using PEI transfection and selection with G418 (InvivoGen, ant-gn-5).
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bioRxiv - Bioengineering 2023Quote: ... A 19-mer 5’ppp-dsRNA positive control for RIG-I activation was obtained from InvivoGen (San Diego, CA; Catalog No. tlrl-3prna).
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bioRxiv - Immunology 2023Quote: Five zebrafish housed in 400-mL beakers containing 200mL sterile tank water were immersed in high molecular weight polyinosine-polycytidylic acid (HMW poly I:C, InvivoGen Cat.# tlrl-pic-5) (50µg/mL final concentration in 200mL tank water ...
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bioRxiv - Immunology 2021Quote: WT and NLRP3-/- iBMDMs were seeded on 150 mm plates at 5×106 cells/plate and on the next day treated with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h. ...
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bioRxiv - Plant Biology 2021Quote: ... Transgenic Col-0 (LUC) plants were selected on 1/2 MS medium supplemented with 50 μM hygromycin B (InvivoGen, Cat No. ant-hg-5). LUC activity was confirmed as described above ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S HexaPro protein (1 µg for the first dose and 5 µg for the booster dose) was mixed with 100 µg of aluminium hydroxide (Invivogen, Cat#vac-alu-250). Serum was collected for analysis on study days 14 ...
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bioRxiv - Immunology 2021Quote: ... Cells reconstituted with pLenti CMVie-IRES-BlastR-based constructs were maintained with addition of 5 µg/ml and 1 µg/ml blasticidin (InvivoGen, Cat. no: ant-bl-05) for HEK293T and iBMDMs ...