Labshake search
Citations for Invivogen :
151 - 200 of 216 citations for High mobility group protein B1 HMGB1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
-
bioRxiv - Immunology 2023Quote: The human monocytic THP-1 cell line (ATCC TIB-202) and the THP-1 DualTM reporter cell (InvivoGen, Thpd-nfis) were culture in RPMI supplemented with 10% heat-inactivated fetal calf serum ...
-
bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
-
bioRxiv - Immunology 2024Quote: Assessment of TLR4 and TLR5 activity was implemented using human embryonic kidney (HEK)-Blue-mTLR4 and HEK-Blue-mTLR5 cells respectively (Invivogen) using a protocol as previously described (Allen et al. ...
-
bioRxiv - Genetics 2020Quote: ... IFN I response was induced by high molecular weight poly(I:C) complexes with LyoVec transfection reagent (Invivogen, tlrl-piclv, 500 ng/ml) or with a combination of poly(I:C)/LyoVec and interferon beta (PeproTech ...
-
bioRxiv - Neuroscience 2021Quote: ... were mixed well and heated to 50°C (or 65-70°C in the case of Invivogen high molecular weight poly I:C) in order to solubilize and melt dsRNA and then cooled to room-temperature to allow re-annealing before freezing in 1 ml aliquots and storing at −20 °C ...
-
bioRxiv - Immunology 2020Quote: ... IFN I response was induced by high molecular weight poly(I:C) complexes with LyoVec transfection reagent (Invivogen, tlrl-piclv, 500 ng/ml), in combination with IFNβ (PeproTech ...
-
bioRxiv - Immunology 2023Quote: ... HEK293-C34 cells were gifted by Y Matsuura at Osaka University and maintained in high-glucose DMEM (Nacalai Tesque) containing 10% FBS and 10 μg/mL blasticidin (solution) (InvivoGen, California, USA), and the exogenous expression of ACE2 and TMPRSS2 was induced by the addition of doxycycline hydrochloride (1 μg/mL ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein translation was inhibited with 50 µg·mL-1 of puromycin (ant-pr-1, Invivogen).
-
bioRxiv - Immunology 2020Quote: ... The lyophilized powder was combined with 300 µg of CpG (Murine CpG, ODN 1826, Porcine, Rabbit, Human CpG ODN 2006 for pig and rabbit, InvivoGen, USA) which has been condensed with Polyethyleneimine (PEI ...
-
bioRxiv - Microbiology 2023Quote: The ADCC reporter assay was performed by using Jurkat-Lucia NFAT-CD16 cells (human FcγRIII, V158) (Invivogen, Cat# jktl-nfat-cd16) as effector cells according to the manufacturer’s instruction with modifications ...
-
bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
-
bioRxiv - Physiology 2021Quote: ... Duodenal or jejunal segments (∼6 cm) were isolated and placed into falcon tubes containing cold DMEM (high glucose, 4.5 g/l) containing Primocin (Invivogen, cat# ant-pm-1). Intestinal epithelial cells were dissociated using a Gentle Cell Dissociation Reagent (Stemcell Technologies ...
-
bioRxiv - Immunology 2023Quote: Five zebrafish housed in 400-mL beakers containing 200mL sterile tank water were immersed in high molecular weight polyinosine-polycytidylic acid (HMW poly I:C, InvivoGen Cat.# tlrl-pic-5) (50µg/mL final concentration in 200mL tank water ...
-
bioRxiv - Immunology 2024Quote: ... mice were intradermally injected with 50 μg of neoantigen or control peptide and/or 100 μg high molecular weight VacciGrade polyinosinic-polycytidylic acid (poly(I:C) (Invivogen Cat. No. vac-pic)) ...
-
bioRxiv - Developmental Biology 2024Quote: ... H9 hESC cells with high expression of sgRNAs (fused with mCherry) were enriched by G418 selection (1000 μg/ml, InvivoGen, ant-gn-1) for 11 days and flow cytometry sorting ...
-
bioRxiv - Biochemistry 2024Quote: ... a fragment of recombinant DNA encoding either residues 21 to 220 of human CD44 or 25 to 224 of murine CD44 were subcloned into an in-house Fc pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
-
bioRxiv - Microbiology 2021Quote: ... Rhesus Adenovirus 52 (RhAd52) (109 viral particles) or protein vaccines (50µg +100µg Adju phos (InvivoGen)) ...
-
bioRxiv - Immunology 2020Quote: ... Slices were cultured overnight in complete media supplemented with 10 µg/mL OVA protein (Invivogen) or PBS ...
-
bioRxiv - Immunology 2022Quote: ... EndoFit chicken egg ovalbumin protein with endotoxin levels <1 EU/mg was purchased from Invivogen for in vivo use at a concentration of 200μg/mice or in vitro use at a concentration ranging from 1000 to 10μg/ml ...
-
bioRxiv - Immunology 2023Quote: Spike proteins (2 μg/dose) were formulated with Alhydrogel 2% (InvivoGen, San Diego, CA, USA) as previously described (20) ...
-
bioRxiv - Microbiology 2023Quote: ... A549-derived cells were transfected with 500 ng of poly(I:C) (a mix of high and low molecular weight, ratio 1:1, InvivoGen, tlrl-picw and tlrl-pic) using lipofectamine 2000 (ThermoFisher ...
-
bioRxiv - Immunology 2020Quote: The cell lines A549-Dual (adenocarcinoma human alveolar basal epithelial cells) and RAW-Lucia ISG (RAW-mouse macrophages) (InvivoGen, San Diego, CA, USA) were cultured in DMEM (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2019Quote: ... virgin female mice were injected intraperitoneally (IP) with a low dose of high molecular weight (HMW) Poly(I:C) dsRNA (InvivoGen, San Diego, CA. Cat# tlrl-pic). At 2.5hrs or 4hrs post-injection ...
-
bioRxiv - Immunology 2022Quote: ... The protein antigen was adjuvanted with AddaVax (1:1 v/v; vac-adx-10, InvivoGen, USA) for immunisation.
-
bioRxiv - Microbiology 2020Quote: ... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...
-
bioRxiv - Microbiology 2023Quote: ... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
-
bioRxiv - Immunology 2022Quote: ... protein samples (0.1 mg/mL) were pre-mixed with alum (10 mg/mL, Alhydrogel®, InvivoGen) at a ratio of 1:10 (protein ...
-
bioRxiv - Microbiology 2022Quote: ... The protocol using HEK-Blue™-hTLR2 and HEK-Blue™-hTLR4 reporter cell lines expressing human TLR2 and TLR4 (InvivoGen, San Diego, CA) was employed according to the manufacturer’s instructions ...
-
Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... boost 4 weeks later of 5 μg recombinant spike protein (aa 14-1209) in 1% alhydrogel (Invivogen) into the right hind limb ...
-
bioRxiv - Bioengineering 2021Quote: ... Purified proteins were tested for endotoxin via HEK-Blue TLR4 reporter cell line (Invivogen, San Diego, CA) and endotoxin levels were confirmed to be less than 0.01 EU/mL ...
-
bioRxiv - Immunology 2021Quote: The capacity of E protein to activate NF-kB was tested by using HEK-TLR2-blue (InvivoGen). In this assay ...
-
bioRxiv - Immunology 2020Quote: ... RBD or OVA proteins were formulated in PBS at a 1:1 ratio with Addavax adjuvant (InvivoGen). C57BL/6 or BALB/c mice were anesthetised by isoflurane inhalation prior to intramuscular injection of 50 μL vaccine in each hind quadriceps ...
-
bioRxiv - Bioengineering 2021Quote: ... The purified proteins were tested for endotoxin using a HEK-Blue™ TLR4 reporter cell line (InvivoGen), and the endotoxin levels were confirmed to be below 0.01 EU mL−1 ...
-
bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
-
bioRxiv - Microbiology 2021Quote: ... the mouse received a final boost intraperitoneally with 100 μg of recombinant N6 protein of A/duck/Zhejiang/D9/2013 (H4N6) adjuvanted with 10 μg of poly (I:C) (Invivogen). Three days after the final boost ...
-
bioRxiv - Biochemistry 2019Quote: ... coli NucC bound to 5’-pApA or cAAA in hanging drop format by mixing protein (8-10 mg/mL) in crystallization buffer plus 0.1 mM 5’-pApA (Invivogen) or cAAA 1:1 with well solution containing 17-24% PEG 3350 ...
-
bioRxiv - Bioengineering 2021Quote: ... of SARS-CoV-2 spike protein (NCBI Accession: YP 009724390.1) were synthesized and cloned into the pFUSE expression vector (InvivoGen). Both constructs contained an AVI-tag and a 6xHis tag at the C-terminus separated by a single G4S linker ...
-
bioRxiv - Immunology 2020Quote: ... right and left axillary LNs) were evaluated 14 days-post IM vaccination with PR8-HA (5μg) protein with Addavax (1:1 ratio; InvivoGen) and 0.5% tattoo ink ...
-
bioRxiv - Immunology 2020Quote: ... were harvested and incubated in 96-well plate (1×104 cells per well) with 250 μg/mL EndoFit Ovalbumin protein (InvivoGen) or 10 μg/mL OVA323–339 (ISQAVHAAHAEINEAGR ...
-
bioRxiv - Immunology 2021Quote: ... BALB/c mice were vaccinated via the intramuscular route with 3 μg of each respective protein with 1:1 mixture of Addavax (Invivogen) in a total volume of 50 μL ...
-
bioRxiv - Microbiology 2020Quote: ... HCoV-OC43 or 15 µg of SARS-CoV spike protein in 150 µL PBS together with 50 µL Addavax (MF59-like squalene adjuvant from InvivoGen) through intraperitoneally injection (i.p.) ...
-
bioRxiv - Cell Biology 2020Quote: We generated dual expression barcoding vectors by inserting pairs of barcoding protein sequences into the pVITRO1-hygro-mcs plasmid (InvivoGen), which contains two multiple cloning sites MCS1 and MCS2 (Supplementary Fig ...
-
bioRxiv - Biophysics 2022Quote: ... H1-Hela+CDHR3 SETD3 KO cells that stably expressed the protein of interest were selected by treatment with 2 μg ml−1 puromycin (InvivoGen) along with non transduced cells as negative controls.
-
bioRxiv - Biochemistry 2021Quote: ... Stable MCF10A cells expressing these proteins were generated from MCF10A ARPIN −/− cells 22 transfected as described above and selected with 10 μg/ml Blasticidin (InvivoGen). Individual clones were picked with cloning rings.
-
bioRxiv - Immunology 2021Quote: ... HEK-293T cells were transfected with the respective S protein expression plasmids for the delta variant (Cat. No. plv-spike-v8, Invivogen) and the omicron variant (A67V ...
-
bioRxiv - Microbiology 2020Quote: ... three BALB/c mice were each injected intraperitoneally (i.p.) with 100 μg of RBD-Fc fusion protein formulated with 500 μg of aluminum hydroxide (Alhydrogel, Invivogen, USA) and 25 μg of CpG (Sangon ...
-
bioRxiv - Microbiology 2022Quote: ... BALB/c mice were vaccinated via the intramuscular route with 3 μg of each respective protein with 1:1 mixture of Addavax (Invivogen) in a total volume of 50 μL ...
-
bioRxiv - Immunology 2022Quote: ... Mice in the RBD + aluminum hydroxide condition received 10 µg of recombinant monomeric SARS-CoV-2 RBD protein formulated with 100 µg of Alhydrogel adjuvant 2% (Invivogen). Mice in the PBS vaccination group received phosphate-buffered saline (PBS ...
-
bioRxiv - Immunology 2023Quote: ... mice were immunized with 50 μg (day 3 analysis) or 100 μg (day 7 analysis) chicken ovalbumin (OVA) protein (InvivoGen) in PBS ...