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Citations for Invivogen :
51 - 100 of 150 citations for CTLA 4 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide ...
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bioRxiv - Physiology 2019Quote: ... and 4 µg/ml blasticidin (InvivoGen). Monoclonal cell lines were tested for robust expression of E2GFP and FaNaC by fluorescence microscopy and patch clamp electrophysiology ...
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bioRxiv - Immunology 2020Quote: Biological activity of laminarin in LamOVA conjugates was analyzed by ELISA using a soluble murine Fc-Dectin-1a receptor (InvivoGen). Hypoallergenicity was analyzed in vitro as a measure of rat basophil leukemia cell (RBL-2H3 ...
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bioRxiv - Biochemistry 2020Quote: ... 17 ECD-10His (extracellular domain) was generated by Gibson cloning and adapted from the commercially available pFUSE-hIgG1-Fc (InvivoGen) vector ...
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bioRxiv - Cancer Biology 2022Quote: ... vector with the KIH strategy “knob” human Fc domain(54) MLA2-arm of the TriTE was converted into a scFab and cloned into a pFUSE (InvivoGen) vector with the KIH strategy “hole” human Fc domain followed by OKT3 ...
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bioRxiv - Microbiology 2020Quote: The DNA sequences of codon-optimized SARS-CoV-2 spike Receptor Binding Domain (S-RBD) and human ACE2 extracellular domain (hACE2-ECD) were cloned into a pFuse-Fc expression vector (Invivogen). A thrombin cleavage sequence was inserted between RBD and Fc to generate a cleavable human Fc tag for future studies ...
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bioRxiv - Immunology 2019Quote: ... KX571250-1324) were cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... three BALB/c mice were each injected intraperitoneally (i.p.) with 100 μg of RBD-Fc fusion protein formulated with 500 μg of aluminum hydroxide (Alhydrogel, Invivogen, USA) and 25 μg of CpG (Sangon ...
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bioRxiv - Immunology 2020Quote: Total spleen cells were cultured at a density of 1 × 106 cells/mL in RPMI 1640 supplemented with 10% fetal calf serum (FCS) with 5 ng/mL LPS (Invivogen). For 4-hydroxytamoxifen (OHTAM ...
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Immunology 2023Quote: ... These Gibson sequences are necessary for cloning of the VH and VL into our rabbit IgG1 expression vectors which are based on the pFUSE-rIgG-Fc (InvivoGen) vectors.75,76 For this second PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... and 4 μg ml-1 puromycin (InvivoGen), and grown at 37 °C in an atmosphere containing 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... Pam3CSK4 (100 µg/mouse; InvivoGen), or Poly(I:C ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of recombinant DNA encoding either residues 21 to 220 of human CD44 or 25 to 224 of murine CD44 were subcloned into an in-house Fc pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Immunology 2021Quote: ... 4 µg cGAMP (Invivogen, San Diego, Californien, USA), 4 µg c-di-UMP (Invivogen ...
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bioRxiv - Immunology 2020Quote: ... for 4 hours and 10 μM nigericin (InvivoGen) for an additional 1 hour ...
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bioRxiv - Immunology 2023Quote: ... and assayed with QUANTI-Luc 4 reagent (Invivogen). Luminescence was read immediately with an Envision system.
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bioRxiv - Immunology 2023Quote: ... selective medium containing Puromycin (4 μg/ml, InvivoGen) was added and after 7 days cells were sorted using FACS Aria (BD ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... and heavy and light chain variable sequences were synthesized as single chain variable fragments (ScFvs) containing a interchain (Light-Heavy) G4S3 linker and cloned into a pFuse-hG1-Fc2 Fc-tagged vector (InVivoGen, San Diego, CA), in-frame between the vector supplied signal sequence (from the human Il-2 protein ...
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bioRxiv - Immunology 2020Quote: ... and selected with 4 μg mL−1 puromycin (InvivoGen) for two weeks.
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bioRxiv - Immunology 2020Quote: ... ovalbumin (OVA) EndoFit (5 μg/mouse) and Alhydrogel adjuvant 2% (alum, 100 μg/mouse) were purchased from Invivogen; recombinant hemagglutinin (rHA ...
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bioRxiv - Microbiology 2020Quote: ... with 50 μl/mouse of AddaVax (Invivogen) as the adjuvant ...
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bioRxiv - Immunology 2023Quote: ... or Poly(I:C) (100 µg/mouse; InvivoGen) in 200 µl PBS for 24 h ...
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bioRxiv - Microbiology 2019Quote: ... Miltenyi, 1000U/mL) and interleukin-4 (IL-4, Miltenyi, 1000U/mL) for 5 days before addition of lipopolysaccharide (LPS, Invivogen, 50 ng/mL) for 2 further days.
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bioRxiv - Cell Biology 2020Quote: ... Stable transformants were selected using 4 μg/mL Blasticidin (Invivogen) and individual colonies were picked ...
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bioRxiv - Immunology 2021Quote: ... 4 µg c-di-UMP (Invivogen, San Diego, Californien, USA) or mock-electroporated using a Gene Pulser Xcell Electroporation instrument (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: HEK-Blue™ IL-4/IL-13 cells (InvivoGen, France) were cultured according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... or the mouse kappa light chain plasmid (InVivoGen). The gBlock DNA and plasmids were cut with the specified enzymes ...
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bioRxiv - Immunology 2020Quote: The VRC01 LC-P2A-HC construct was PCR-amplified from engineered C57BL/6J mouse cDNA and cloned between the promoter and constant regions of a mouse IgG2b heavy chain expression vector (InvivoGen, #pfuse-mchg2b) using Gibson Assembly ...
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bioRxiv - Immunology 2019Quote: ... a Mouse TLR Agonist kit was purchased from Invivogen and BMDMs were treated with each ligand for 16 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µM digitonin) with or without 7 µM 2’,3’-cGAMP (Invivogen). Cells were further washed in PBS and incubated in culture medium until collection ...
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bioRxiv - Systems Biology 2023Quote: ... TLR4 agonist at 4 ng/mL (LPS-B5, Invivogen, San Diego, CA), BTC at 40 ng/mL (human recombinant Betacellulin protein ...
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bioRxiv - Immunology 2023Quote: ... cells were transferred to selective medium containing 4 µg/mL Blasticidin (Invivogen) (MC38-B2m-/- transduced with Her2/neu ...
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bioRxiv - Cell Biology 2022Quote: ... ASC-expressing RAW264.7 mouse macrophages (RAW-ASC; InvivoGen raw-asc) are engineered to stably express murine ASC ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies used: Mouse anti-HA antibody (InvivoGen, 1:1000), rabbit anti-ACP (1:2000) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IFN-α was measured by a bioluminescence kit (InvivoGen). All assays were performed on cell free supernatants according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... or 200 µg of isotype control antibody (mouse IgG2a, Invivogen) three times at days 6 ...
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bioRxiv - Immunology 2023Quote: ... mice were immunised with VacOva (vac-pova, InvivoGen; 50μg/mouse) and LPS (L6529 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse STING activator DMAXX and cGAMP were purchased from InvivoGen.
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bioRxiv - Cancer Biology 2023Quote: ... Mouse NIK expression plasmid was purchased from Invivogen (pUNO1-mMap3k14). Wild-type human NIK and the NIK-K429/430A mutant plasmids were a gift from Prof ...
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bioRxiv - Immunology 2020Quote: ... the cells were primed for 4 h with 500 ng/mL ultrapure LPS (InvivoGen). The supernatant was discharged and macrophages were infected with trypomastigote forms of T ...
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bioRxiv - Immunology 2019Quote: ... HBV Pol and HBcAg in pVAC1 vector (Invivogen, 50 μg/mouse ea.); the group of mice immunized with empty vector VLV received empty pVAC1 vector (150 μg/mouse) ...
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bioRxiv - Immunology 2020Quote: ... mouse RAW macrophages (RAW-Lucia ISG, RAW-Lucia ISG-KO-cGAS, Invivogen) were cultured in DMEM (Thermo Fisher cat ...
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bioRxiv - Immunology 2023Quote: ... LPS-primed mouse BMDMs were transfected with 2 μg poly(dA:dT) (InvivoGen) using Lipofectamine 3000 and incubated for 6 h.
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bioRxiv - Immunology 2023Quote: ... mouse NIH/3T3 fibroblasts were transfected with NotI-linearized pUNO1 vectors (Invivogen) encoding human BAFF (CD257 ...
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bioRxiv - Biochemistry 2023Quote: ... and 50 µL of the working solution of the QUANTI1Luc 4 Lucia/Gaussia reagent (Invivogen) was added ...
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bioRxiv - Immunology 2022Quote: 20 µg/mouse poly(I:C) (HMW) VacciGrade™ (Invivogen, San Diego, CA, USA) and 20 µg/mouse 3pRNA (synthesized by AG Hartmann ...