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Citations for Invivogen :
501 - 550 of 1344 citations for 6 Chloro 2 methyl 1 2 4 triazolo 4 3 b pyridazin 3 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS media was replaced with fresh media and 24 hours later U2OS infected cells were selected with 2 µg/ml of puromycin (InvivoGen) for 72 hours.
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bioRxiv - Neuroscience 2021Quote: ... were a kind gift of David Pla-Martin and Francesc Palau 21 and were grown in growth medium containing 2 μg/ml puromycin (InvivoGen).
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bioRxiv - Immunology 2021Quote: ... by staining and acquiring control samples consisting of aliquots of fixed and frozen blood leukocytes from a healthy macaque after ex vivo stimulation of whole blood for 2 hours with three TLR ligands: LPS (LPS E. coli 0111: B4, Invivogen) at 1 μg/mL ...
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bioRxiv - Genetics 2019Quote: ... The untransduced cells and serially diluted virus-treated cells were cultured in the presence of 2 μg/ml puromycin or 20 μg/ml blasticidin S (InvivoGen). When almost all of the untransduced cells had died ...
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bioRxiv - Immunology 2020Quote: ... The immunization mixture contained 13 μg of SARS-CoV-2-S-I53-50NPs (equal to 10 μg of SARS-CoV-2-S) adjuvanted with 50 μg of polyinosinic-polycytidylic acid (Poly-IC; Invivogen). Blood was collected at weeks-1,2 ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were discarded before staining with 50 μL of 2 μg/mL secondary AF488 goat anti rabbit (goat IgG H+L, Invivogen) in cytometry buffer for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... MHCI-specific ovalbumin SIINFEKL/OVA257-264 or MHCII-specific ovalbumin peptide OVA323-339 were added at 2 μg/ml (Invivogen). Phorbol 12-myristate 13-acetate (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide plasmid and PB transposase were lipofectamin transfected into parental SunTag cell lines and selected by puromycin (2 µg/ml, Invivogen) treatment ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells that stably express human ACE2 (ACE2-293T) were cultivated in the presence of 2 μg/ml puromycin (Invivogen). Caco2 (human epithelial colorectal adenocarcinoma ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed bone marrow cells were cultured in RPMI supplemented with 10% FBS with or without a bacterial agonist cocktail (2ug/mL Pam3CSK4 (TLR1/2 agonist, InvivoGen), 1 ug/mL FSL-1 (TLR2/6 agonist ...
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bioRxiv - Immunology 2023Quote: ... followed by the medium being replaced with fresh IFNγ (5 ng/ml) containing media for 2 h before treatment with indicated stimulations: MDP (100 μM, Invivogen), iE-DAP (100 μM ...
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bioRxiv - Genetics 2023Quote: ... 5% CO 2 prior to stimulation under the same conditions with the following reagents and concentrations - Pam3Csk4 (Invivogen, Lot no.), LPS (Invivogen ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were expanded to a surface-treated T-75 flask and treated with 2 µg/mL of puromycin (Invivogen) for 2 weeks for selection of the transduced cells to generate the cell line expressing hM3D(Gq) ...
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bioRxiv - Microbiology 2023Quote: ... S10-3 WT cells were transduced with pTRIPZ-Rab5/7/11-GFP or EGFP-LAMP1 and selected in cDMEM supplemented with 2 μg/ml puromycin (Invivogen).
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bioRxiv - Molecular Biology 2023Quote: ... cells were transferred to a 10 cm dish the day after transfection and selection with Puromycin (2 μg/mL, InvivoGen) was started within 48 h of transfection ...
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bioRxiv - Microbiology 2024Quote: ... RD-SCARB2-KO20 and RD-ΔEXT1+hSCARB254 cells were maintained in DMEM supplemented with 10 µg/ml puromycin (58-58-2, InvivoGen). All infected cells were maintained in media supplemented with 2.5% FBS ...
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bioRxiv - Genomics 2024Quote: ... A total of 17,114 BFP+ cells were replated into a half deep 96 well plate (Applikon Biotechnology) and allowed to expand with the addition of 2 μg/mL doxycycline and 100 μg/mL blasticidin (Invivogen) to select for functional landing pad cells ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μg/ml Hygromycin B and 50 μg/ml Zeocin (all from InvivoGen). Primary human bone marrow-derived mesenchymal stem cells were obtained from ATCC (PCS-500-012) ...
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bioRxiv - Cell Biology 2022Quote: ... with 10% fetal bovine serum (FBS) and 10 µg/mL Hygromycin B (InvivoGen) at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The positive clones (iR9X2-ESC) selected by hygromycin B (150 μg/ mL, Invivogen) were further cultured in ES medium supplemented with doxycycline (1 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... LPS-stimulated B cells were treated with 100 nM of Bafilomycin A1 (InvivoGen) for 3 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 μg/ml G418 or the indicated amount of Hygromycin B Gold (InvivoGen) was added for selection ...
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bioRxiv - Bioengineering 2024Quote: ... this clone was used to generate a stable pool harboring the human glycosyltransferase gene ST6GAL1 via transfection with a plasmid containing the coding sequence of human beta-galactoside alpha-2,6-sialyltransferase 1 isoform a (hSTGAL1) (GenBank NP_001340845.1) and expressed from a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a derivative of pcDNA3.1(+ ...
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bioRxiv - Microbiology 2021Quote: ... vaccines were in PBS and adjuvantized with one volume of ADDavax (Invivogen). All the mice received 2 vaccine doses at 2 weeks of interval (Day 0 and Day 14 ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... where n=2) were immunized with the indicated immunogen in 100 μL of 50% v/v of AddaVax™ adjuvant (Invivogen) and boosted with adjuvant on Day 14 ...
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bioRxiv - Immunology 2021Quote: ... Mice were immunized with SARS-CoV-2 spike protein at 0.5 μg/50μl alum (InvivoGen, stock concentration aluminum 10 μg/μl)/mouse intraperitoneally on days 0 and 14 (Fig 1A) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant IgA1 produced in ExpiCHO-S cells as described above was purified using protein M agarose beads (Cat# gel-pdm-2, InvivoGen US) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... FSL1 (TLR2/6; tlrl-fsl; Invivogen, 100 ng/ml), LPS 0111:B4 (TLR4 ...
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bioRxiv - Microbiology 2023Quote: ... or 6 ng/ml blasticidin (InvivoGen, Cat# ant-bl). Single-cell clones were isolated by the limiting dilution of the drug-resistant cell pool and expanded ...
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bioRxiv - Cancer Biology 2023Quote: ... and Mycoplasma testing within 6 months of use (InVivoGen). Cell lines were maintained in culture at 37°C in 5% CO2.
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bioRxiv - Cell Biology 2022Quote: ... and were selected for ten to fourteen days with a selection medium (contains 1 μg/ml Blasticidin [Invivogen, #ant-bl], 200 μg/ml Hygromycin B gold [Invivogen, #ant-hg] and 1μg/mL Puromycin). Twenty four clones were screened for the mCherry signal visually with fluorescent microscope ...
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bioRxiv - Molecular Biology 2019Quote: ... diluted and reseeded in the presence of 320 µg/ml hygromycin B Gold (InvivoGen). In 48 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Selection of stably expressing cells were performed using 150μg/mL Hygromycin B Gold (InvivoGen) as per kill curve (data not shown) ...
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bioRxiv - Immunology 2021Quote: ... the positive clones (iRunx1-Lhx2-ESC) selected by hygromycin B (150 μg/ mL, Invivogen) were further cultured in ES medium supplemented with doxycycline (1 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were selected with 100 µg/mL Hygromycin B Gold (Invivogen, #ant-hg-5). Selected clones were isolated and GFP expression was confirmed by Western blotting and flow cytometry.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 250 μg heroin-TT conjugate was mixed with 50 μg of phosphorothioate-modified CpG ODN 1826 (Eurofins Genomics, Louisville, KY) and 0.75 mg Alhydrogel adjuvant 2% (InvivoGen, San Diego, CA) for 30 min and then refrigerated for 24 hours prior to IP administration ...
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bioRxiv - Microbiology 2022Quote: ... 293-ACE2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 200 µg/mL G418 (Invivogen #ant-gn-2). HEK-Blue 293 hACE2-TMPRSS2 cells (Invivogen #hkb-hace2tpsa ...
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bioRxiv - Immunology 2023Quote: ... Guide-RNAs were stably transduced in SpCas9-expressing DC 2.4 cells using the LentiGuide-Neo vector and selected with 500 ug/mL of G418 (InvivoGen #108321-42-2). LentiGuide-Neo was a gift from Caroline Goujon (Addgene plasmid # 139449 ...
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bioRxiv - Immunology 2023Quote: ... Intraperitoneal sensitization: Mice were immunized intraperitoneally with 250 μg OVA and 250 μL Alhydrogel 2% (aluminum hydroxide [alum], InvivoGen, San Diego, CA), 40 μg CpG ...
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bioRxiv - Neuroscience 2021Quote: ... one from Invivogen of high molecular weight (HMW) (1.5 kb – 8 kb) and one from Invivogen of low molecular weight (LMW ...
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bioRxiv - Immunology 2021Quote: ... one day after transduction the cells were cultured in 5µg/ml puromycin(Invivogen) and 5µg/ml zeocin(ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... one day after transduction the cells were cultured in 5μg/ml puromycin(Invivogen) and 5μg/ml zeocin(ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... C57BL/6 mice were treated with 200ug poly(I:C) (Invivogen) for 2 days ...
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bioRxiv - Microbiology 2019Quote: ... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
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bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...
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bioRxiv - Immunology 2020Quote: Undifferentiated THP-1 and PMA-differentiated cells (2 × 105 cells) in biological duplicates per condition were stimulated with TLR agonists- CL075 (TLR8; tlrl-c75; Invivogen, 5 μg/ml), CpG2006 (TLR9 ...